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Protein Tyrosine Phosphatase, Receptor Type C Peptide 1

Antibody: CPTC-PTPRC-1
Background

Catalog Number:

CPTC-PTPRC-1

RRID:

AB_2888967

Target Antigen:

Protein Tyrosine Phosphatase, Receptor Type C Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

01/08/2025

Antigen Recognition(s):

Peptide

Thematic Panel(s):

Immuno-Oncology (IO)

External Links
Purchase
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (2.0 MB)

CPTC-PTPRC-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-PTPRC-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-1172) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html) Click image to enlarge

CPTC-PTPRC-1 IMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-PTPRC-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-1172)

Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)


Characterization SOP Files

  NCI 60 Protein Array

CPTC-PTPRC-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

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Antibody: CPTC-PTPRC-2
Background

Catalog Number:

CPTC-PTPRC-2

RRID:

AB_2888968

Target Antigen:

Protein Tyrosine Phosphatase, Receptor Type C Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

01/02/2026

Antigen Recognition(s):

Peptide, Endogenous

Thematic Panel(s):

Immuno-Oncology (IO)

External Links
Purchase
External Links
Publications
Characterization Data [Compare Characterization Data]
  Flow Cytometry
Click to enlarge image Flow cytometric analysis of Receptor-type tyrosine-protein phosphatase C (PTPRC) expression using CPTC-PTPRC-2 rabbit antibody. CCRF-CEM cells were fixed, permeabilized, and then stained with CPTC-PTPRC-2 (solid green) or concentration-matched rabbit isotype control (dashed green) antibodies. HL-60 cells were fixed, permeabilized, and then stained with CPTC-PTPRC-2 (solid blue) or concentration-matched rabbit isotype control (dashed blue) antibodies. A BV421 conjugated goat anti-rabbit IgG was used as a secondary antibody. All data were analyzed using FlowJo.  Click image to enlarge

CPTC-PTPRC-2 Flow Cytometry

Result: Positive

Flow cytometric analysis of Receptor-type tyrosine-protein phosphatase C (PTPRC) expression using CPTC-PTPRC-2 rabbit antibody. CCRF-CEM cells were fixed, permeabilized, and then stained with CPTC-PTPRC-2 (solid green) or concentration-matched rabbit isotype control (dashed green) antibodies. HL-60 cells were fixed, permeabilized, and then stained with CPTC-PTPRC-2 (solid blue) or concentration-matched rabbit isotype control (dashed blue) antibodies. A BV421 conjugated goat anti-rabbit IgG was used as a secondary antibody. All data were analyzed using FlowJo. 


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (194.7 KB)

CPTC-PTPRC-2 IHC evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-PTPRC-2 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5946) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool. Click image to enlarge

CPTC-PTPRC-2 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-PTPRC-2 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5946)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool.


Characterization SOP Files

  Immunofluorescence
Click to enlarge image Immunofluorescence staining using CPTC-PTPRC-2 as primary antibody (pink-violet) against HL-60 and CCRF-CEM cells. CCRF-CEM cells show PTPRC protein subcellular localization in the cell membrane. Click image to enlarge

CPTC-PTPRC-2 Immunofluorescence

Result: Positive

Immunofluorescence staining using CPTC-PTPRC-2 as primary antibody (pink-violet) against HL-60 and CCRF-CEM cells. CCRF-CEM cells show PTPRC protein subcellular localization in the cell membrane.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Single cell western blot using CPTC-PTPRC-2 as a primary antibody against HL-60 and CCRF-CEM cell lysates. Relative expression of total PTPRC (A). Percentage of cells that express PTPRC (B). Average expression of PTPRC protein per cell (C). All data is normalized to β-tubulin expression. Click image to enlarge

CPTC-PTPRC-2 Single Cell Western Blot (Cell Lysate)

Result: Positive

Single cell western blot using CPTC-PTPRC-2 as a primary antibody against HL-60 and CCRF-CEM cell lysates. Relative expression of total PTPRC (A). Percentage of cells that express PTPRC (B). Average expression of PTPRC protein per cell (C). All data is normalized to β-tubulin expression.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-PTPRC-2 as primary antibody against the whole lysates of CCRF-CEM, SR, MOLT-4, HL-60, RPMI-8226, and EKVX. The antibody detected the target protein (~147.5 KDa) in CCRF-CEM cell lysate. Click image to enlarge

CPTC-PTPRC-2 Western Blot (Cell Lysate)

Result: Positive

Western blot using CPTC-PTPRC-2 as primary antibody against the whole lysates of CCRF-CEM, SR, MOLT-4, HL-60, RPMI-8226, and EKVX. The antibody detected the target protein (~147.5 KDa) in CCRF-CEM cell lysate.


Characterization SOP Files

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Antigen: Protein Tyrosine Phosphatase, Receptor Type C Peptide 1
Background

NCI Identification Number:

00252

Antigen Name:

Protein Tyrosine Phosphatase, Receptor Type C Peptide 1

CPTC Name:

CPTC-PTPRC Peptide 1

Aliases:

Protein Tyrosine Phosphatase, Receptor Type C; CD45 Antigen; CD45; L-CA; T200; Protein Tyrosine Phosphatase, Receptor Type, C Polypeptide; Receptor-Type Tyrosine-Protein Phosphatase C; T200 Leukocyte Common Antigen; Leukocyte Common Antigen; T200 Glycoprotein; EC 3.1.3.48; GP180;
CD45R; B220; LCA; LY5

Function:

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.

PTPRC (Protein Tyrosine Phosphatase, Receptor Type C) is a Protein Coding gene. Diseases associated with PTPRC include Severe Combined Immunodeficiency, T Cell-Negative, B-Cell/Natural Killer-Cell Positive and Hepatitis C Virus. Among its related pathways are TCR signaling (REACTOME) and TCR signaling in naive CD4+ T cells. GO annotations related to this gene include protein kinase binding and phosphatase activity. An important paralog of this gene is PTPRS.

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).

Chromosomal Localization:

1q31.3-q32.1

Accession Number:

NP_001254727.1

UniProt Accession Number:

P08575

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

LFLAEFQSIPR

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1210

Last Updated:

02/26/2018

Links

Characterization Data

SOPs

No SOPs available.

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