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Peroxiredoxin 4 Peptide 2

Antibody: CPTC-PRDX4-5
Background

Catalog Number:

CPTC-PRDX4-5

RRID:

AB_2877662

Target Antigen:

Peroxiredoxin 4 Peptide 2

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (624.0 KB)

CPTC-PRDX4-5 evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of breast cancer showing cytoplasmic staining using Antibody CPTC- CPTC-PRDX4-5. Titer: 1:20000 Click image to enlarge

CPTC-PRDX4-5 IHC Tissue

Result: Positive

Tissue Micro-Array (TMA) core of breast cancer showing cytoplasmic staining using Antibody CPTC- CPTC-PRDX4-5. Titer: 1:20000


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-PRDX4-5 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-714) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from plasma. Click image to enlarge

CPTC-PRDX4-5 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-PRDX4-5 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-714)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from plasma.


Characterization SOP Files

  Immunofluorescence

CPTC-PRDX4-5 Immunofluorescence

Result: Negative

Immunofluorescence staining of MCF7 cells using CPTC-PRDX4-5 antibody. PRDX4 protein expression was not detected.


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (624.0 KB)

CPTC-PRDX4-5 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Peptide coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-PRDX4-5 Indirect ELISA (peptide)

Result: Positive

Indirect ELISA (ie, binding of Antibody to Peptide coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to full-length Antigen coated on plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-PRDX4-5 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to full-length Antigen coated on plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-PRDX4-5 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-PRDX4-5 NCI 60 Protein Array

Result: Positive

Protein Array in which CPTC-PRDX4-5 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - HPA - tissue or cell lysate
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). Single band corresponding to the predicted size in kDa (+/-20%). Analysis performed using a standard panel of samples. Antibody dilution: 1:500 Click image to enlarge

CPTC-PRDX4-5 evaluation by the Human Protein Atlas

Result: Presumed Positive (with additional bands)

Results provided by the Human Protein Atlas (www.proteinatlas.org).

Single band corresponding to the predicted size in kDa (+/-20%).
Analysis performed using a standard panel of samples. Antibody dilution: 1:500


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-PRDX4-5 as primary Ab against full-length recombinant Ag 10604 (lane 2). Also included are molecular wt. standards (lane 1) and the PRDX4-5 Ab as positive control (lane 3). Click image to enlarge

CPTC-PRDX4-5 Western Blot

Result: Positive

Western Blot using CPTC-PRDX4-5 as primary Ab against full-length recombinant Ag 10604 (lane 2). Also included are molecular wt. standards (lane 1) and the PRDX4-5 Ab as positive control (lane 3).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-PRDX4-5 as primary antibody against human Peroxiredoxin 4 (PRDX4) recombinant protein (lane 2). Molecular weight standards are also included (lane 1). Expected molecular weight – 30.4 kDa. Click image to enlarge

CPTC-PRDX4-5 Western Blot (Recombinant Protein)

Result: Positive

Western Blot using CPTC-PRDX4-5 as primary antibody against human Peroxiredoxin 4 (PRDX4) recombinant protein (lane 2). Molecular weight standards are also included (lane 1). Expected molecular weight – 30.4 kDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-PRDX4-5 as primary Ab against lysates from six breast cancer cell lines from the NCI60 cell line collection (lanes 2-7). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-PRDX4-5 NCI60 Western blot

Result: Positive

Western Blot using CPTC-PRDX4-5 as primary Ab against lysates from six breast cancer cell lines from the NCI60 cell line collection (lanes 2-7). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

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Antigen: Peroxiredoxin 4 Peptide 2
Background

NCI Identification Number:

00049

Antigen Name:

Peroxiredoxin 4 Peptide 2

CPTC Name:

CPTC-PRDX4 Peptide 2

Aliases:

AOE37-2; Antioxidant Enzyme AOE372; Peroxiredoxin IV; Thioredoxin Peroxidase AO372; Thioredoxin-Dependent Peroxide Reductase A0372; EC 1.11.1.15; PRX-4; Prx-IV; Thioredoxin Peroxidase (Antioxidant Enzyme)

Function:

The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is
localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein
has been found to play a regulatory role in the activation of the transcription factor NF-kappaB.

Probably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a
modulation of I-kappa-B-alpha phosphorylation.

Chromosomal Localization:

Accession Number:

NP_006397.1

UniProt Accession Number:

Q13162

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

LVQAFQYTDK

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1100

Last Updated:

06/21/2013

Links

Characterization Data

SOPs

No SOPs available.

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