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Peroxiredoxin 4 Peptide 1

Antibody: CPTC-PRDX4-4
Background

Catalog Number:

CPTC-PRDX4-4

Target Antigen:

Peroxiredoxin 4 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org). (518.4 KB)

CPTC-PRDX4-4 evaluation by the Human Protein Atlas

Result: Positive

This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-PRDX4-4 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-713) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from plasma. Click image to enlarge

CPTC-PRDX4-4 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-PRDX4-4 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-713)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from plasma.


Characterization SOP Files

  Immunofluorescence

CPTC-PRDX4-4 Immunofluorescence

Result: Negative

Immunofluorescence staining of MCF7 cells using CPTC-PRDX4-4 antibody. PRDX4 protein expression was not detected.


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (518.4 KB)

CPTC-PRDX4-4 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to full-length Antigen coated on plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-PRDX4-4 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to full-length Antigen coated on plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-PRDX4-4 as primary Ab against full-length recombinant Ag 10604 (lane 2). Also included are molecular wt. standards (lane 1) and the PRDX4-4 Ab as positive control (lane 3). Click image to enlarge

CPTC-PRDX4-4 Western blot

Result: Positive

Western Blot using CPTC-PRDX4-4 as primary Ab against full-length recombinant Ag 10604 (lane 2). Also included are molecular wt. standards (lane 1) and the PRDX4-4 Ab as positive control (lane 3).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-PRDX4-4 as primary Ab against lysates from six breast cancer cell lines from the NCI60 cell line collection (lanes 2-7). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-PRDX4-4 NCI60 Western blot

Result: Positive

Western Blot using CPTC-PRDX4-4 as primary Ab against lysates from six breast cancer cell lines from the NCI60 cell line collection (lanes 2-7). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

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Antigen: Peroxiredoxin 4 Peptide 1
Background

NCI Identification Number:

00048

Antigen Name:

Peroxiredoxin 4 Peptide 1

CPTC Name:

CPTC-PRDX4 Peptide 1

Aliases:

AOE37-2; Antioxidant Enzyme AOE372; Peroxiredoxin IV; Thioredoxin Peroxidase AO372; Thioredoxin-Dependent Peroxide Reductase A0372; EC 1.11.1.15; PRX-4; Prx-IV; Thioredoxin Peroxidase (Antioxidant Enzyme)

Function:

The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is
localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein
has been found to play a regulatory role in the activation of the transcription factor NF-kappaB.

Probably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a
modulation of I-kappa-B-alpha phosphorylation.

Chromosomal Localization:

Accession Number:

NP_006397.1

UniProt Accession Number:

Q13162

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

QITLNDLPVGR

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1210

Last Updated:

06/21/2013

Links

Characterization Data

SOPs

No SOPs available.

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