PRINT

Programmed Cell Death 1 Ligand 2 Peptide 1

Antibody: CPTC-PDCD1LG2-1
Background

Catalog Number:

CPTC-PDCD1LG2-1

RRID:

AB_2889058

Target Antigen:

Programmed Cell Death 1 Ligand 2 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

Thematic Panel(s):

Immuno-Oncology (IO)

External Links
Purchase
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (346.7 KB)

CPTC-PDCD1LG2-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC Tissue

CTPC-PDCD1LG2-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-PDCD1LG2-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5986) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool. Click image to enlarge

CPTC-PDCD1LG2-1 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-PDCD1LG2-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5986)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool.


Characterization SOP Files

  Immunofluorescence

CPTC-PDCD1LG2-1 Immunofluorescence

Result: Negative

Immunofluorescence staining of human cell lines Jurkat, MCF7 and NCI H226 with CPTC-PDCD1LG2-1 Ab shows no localization of PDCD1LG2 protein.


Characterization SOP Files

  NCI 60 Protein Array

CTPC-PDCD1LG2-1 NCI60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-PDCD1LG2-1 as primary antibody against human programmed cell death 1 ligand 2 (PDCD1LG2) recombinant protein (lane 2). Expected molecular weight - 30.8 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-PDCD1LG2-1 Western Blot (recombinant protein)

Result: Positive

Western blot using CPTC-PDCD1LG2-1 as primary antibody against human programmed cell death 1 ligand 2 (PDCD1LG2) recombinant protein (lane 2). Expected molecular weight - 30.8 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-PDCD1LG2-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) cell lysates. Expected molecular weight - 31 kDa. Molecular weight standards are also included (lane 1). Data is positive for buffy coat. Data is negative for remaining cell lines. Click image to enlarge

CPTC-PDCD1LG2-1 Automated Western Blot (Cell Lysate)

Result: Positive

Automated western blot using CPTC-PDCD1LG2-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) cell lysates. Expected molecular weight - 31 kDa. Molecular weight standards are also included (lane 1). Data is positive for buffy coat. Data is negative for remaining cell lines.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-PDCD1LG2-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5) and NCI H226 (lane 6) cell lysates. Expected molecular weight 31 kDa. Molecular weight standards (MW Stds.) are also included (lane 1). Positive for cell line NCI H226. Negative/inconsistent results for the other cell lines. Click image to enlarge

CPTC-PDCD1LG2-1 Western Blot (Cell Lysate)

Result: Positive

Western blot using CPTC-PDCD1LG2-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5) and NCI H226 (lane 6) cell lysates. Expected molecular weight 31 kDa. Molecular weight standards (MW Stds.) are also included (lane 1). Positive for cell line NCI H226. Negative/inconsistent results for the other cell lines.


Characterization SOP Files

[top]

Antigen: Programmed Cell Death 1 Ligand 2 Peptide 1
Background

NCI Identification Number:

00385

Antigen Name:

Programmed Cell Death 1 Ligand 2 Peptide 1

CPTC Name:

CPTC-PDCD1LG2 Peptide 1

Aliases:

Programmed Cell Death 1 Ligand 2; CD273; PD-L2; B7-DC; PDL2; B7 Dendritic Cell Molecule; Programmed Death Ligand 2; Butyrophilin B7-DC; PDCD1 Ligand 2; BA574F11.2; PDCD1L2; B7DC; Btdc; PD-1-Ligand 2; PD-1 Ligand 2; CD273 Antigen; PDCD1LG2

Function:

PDCD1LG2 (Programmed Cell Death 1 Ligand 2) is a Protein Coding gene. Diseases associated with PDCD1LG2 include Cysticercosis and Testicular Lymphoma. Among its related pathways are Innate Immune System and Class I MHC mediated antigen processing and presentation. An important paralog of this gene is CD274.Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production (By similarity).

Chromosomal Localization:

9p24.1

Accession Number:

NP_079515.2

UniProt Accession Number:

Q9BQ51

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

ATLLEEQLPLGK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1320

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

[top]

Don't have Adobe Reader™?

Get it for free at Adobe.com

  [top]