Background
Catalog Number:
CPTC-PDCD1-1
Target Antigen:
Programmed Cell Death 1 Peptide 1
Isotype:
IgG
Species:
Rabbit Recombinant Cloned Antibody
Last Updated:
07/03/2024
Antigen Recognition(s):
Peptide, Endogenous
External Links
- CPTAC Assay Portal CPTC-PDCD1-1
Purchase
- DSHB CPTC-PDCD1-1
Characterization Data [Compare Characterization Data]
Immuno-MRM
CPTC-PDCD1-1 iMRM (Frozen Tissue)
Result: Positive
Immuno-MRM chromatogram of CPTC-PDCD1-1antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6208)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue
Characterization SOP Files
NCI 60 Protein Array
CPTC-PDCD1-1 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-PDCD1-1 Western Blot Recombinant Protein
Result: Negative
Western blot using CPTC-PDCD1-1 as primary antibody against human programmed cell death 1 (PDCD1) recombinant protein (lane 2). Expected molecular weight - 29.2 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-PDCD1-1 Western Blot Cell Lysate
Result: Negative
Western blot using CPTC-PDCD1-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and NCI-H226 (lane 6) whole cell lysates. Expected molecular weight - 31.6 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-PDCD1-1 Automated Western Blot Cell Lysate
Result: Negative
Automated western blot using CPTC-PDCD1-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 31.6 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Background
NCI Identification Number:
00498
Antigen Name:
Programmed Cell Death 1 Peptide 1
CPTC Name:
CPTC-PDCD1 Peptide 1
Aliases:
Programmed Cell Death 1; PD1; Systemic Lupus Erythematosus Susceptibility 2; Programmed Cell Death Protein 1; Protein PD-1; CD279; HPD-1; HSLE1; PD-1; Programmed Cell Death 1 Protein; CD279 Antigen; SLEB2; HPD-L; PDCD1
Function:
Programmed cell death protein 1 (PDCD1) is an immune-inhibitory receptor expressed in activated T cells; it is involved in the regulation of T-cell functions, including those of effector CD8+ T cells. In addition, this protein can also promote the differentiation of CD4+ T cells into T regulatory cells. PDCD1 is expressed in many types of tumors including melanomas, and has demonstrated to play a role in anti-tumor immunity. Moreover, this protein has been shown to be involved in safeguarding against autoimmunity, however, it can also contribute to the inhibition of effective anti-tumor and anti-microbial immunity.PDCD1 (Programmed Cell Death 1) is a Protein Coding gene. Diseases associated with PDCD1 include Systemic Lupus Erythematosus 2 and Multiple Sclerosis. Among its related pathways are IgA-Producing B Cells in the Intestine and Translocation of ZAP-70 to Immunological synapse. Gene Ontology (GO) annotations related to this gene include obsolete signal transducer activity.Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed:21276005). Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (By similarity). Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta (By similarity).The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28951311). The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function (PubMed:28951311). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (PubMed:22658127, PubMed:25034862, PubMed:25399552).
Chromosomal Localization:
2q37.3
Accession Number:
NP_005009.2
UniProt Accession Number:
Q15116
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
LAAFPEDR
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
880
Last Updated:
07/15/2021
Links
- EnsEMBL - EnsEMBL PDCD1
- Entrez - Entrez PDCD1
- Uniprot - Uniprot Q15116
Characterization Data
SOPs
No SOPs available.
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