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Proliferating Cell Nuclear Antigen Peptide 1

Antibody: CPTC-PCNA-1
Background

Catalog Number:

CPTC-PCNA-1

RRID:

AB_2617307

Target Antigen:

Proliferating Cell Nuclear Antigen Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

07/03/2024

Antigen Recognition(s):

Peptide, Endogenous

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (352.7 KB)

CPTC-PCNA-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-PCNA-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-722) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from plasma. Data collected from cell lysates are available on the CPTAC assay portal (https://assays.cancer.gov/CPTAC-3242) Click image to enlarge

CPTC-PCNA-1 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-PCNA-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-722)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from plasma. Data collected from cell lysates are available on the CPTAC assay portal (https://assays.cancer.gov/CPTAC-3242)


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-PCNA-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6236) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue Click image to enlarge

CPTC-PCNA-1 iMRM (Frozen Tissue)

Result: Positive

Immuno-MRM chromatogram of CPTC-PCNA-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6236)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-PCNA-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6236) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue Click image to enlarge

CPTC-PCNA-1 iMRM (Frozen Tissue)

Result: Positive

Immuno-MRM chromatogram of CPTC-PCNA-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6236)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue


Characterization SOP Files

  Immunofluorescence - HPA
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line THP-1 shows localization to nucleoplasm. Human assay: THP-1 fixed with PFA, dilution: 1:100 Human assay: U2OS fixed with PFA, dilution: 1:100 Click image to enlarge

CPTC-PCNA-1 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line THP-1 shows localization to nucleoplasm. Human assay: THP-1 fixed with PFA, dilution: 1:100
Human assay: U2OS fixed with PFA, dilution: 1:100


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-PCNA-1 Indirect ELISA (peptide)

Result: Positive

Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-PCNA-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate
Click to enlarge image Western blot using CPTC-PCNA-1 as primary antibody against PCNA HEK293T cell transient overexpression lysate. The expected molecular weight is 28.8 kDa. Click image to enlarge

CPTC-PCNA-1 Western Blot (Over-Expressed Lysate)

Result: Presumed Positive (with additional bands)

Western blot using CPTC-PCNA-1 as primary antibody against PCNA HEK293T cell transient overexpression lysate. The expected molecular weight is 28.8 kDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-PCNA-1 Simple Western (cell lysates)

Result: Negative

Automated WB (Simple Western) using CPTC-PCNA-1 as primary antibody against the whole lysate of PBMC, HeLa, Jurkat, A549, MCF7 and H226. Expected MW is 28.8 KDa. The antibody does not recognize the target in the tested cell lysates. The same cell lysates were probed with an anti-CytC antibody.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-PCNA-1 Western Blot (Cell Lysate)

Result: Negative

Western blot using CPTC-PCNA-1 as primary antibody against human PBMC (2), HeLa (3), Jurkat (4), A549 (5), MCF7 (6) and H226 (7) whole cell lysates. The expected molecular weight is 28.8 kDa. Cytochrome C was used as a loading control.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-PCNA-1 Immunoprecipitation

Result: Negative

Immuno-precipitation performed using CPTC-PCNA-1 as capture antibody against the over-expressed lysate of PCNA (Myc-tagged). Eluates were tested in automated western blot using the an anti-Myc tag as detection antibody. The target protein was not pulled down.


Characterization SOP Files

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Antigen: Proliferating Cell Nuclear Antigen Peptide 1
Background

NCI Identification Number:

00035

Antigen Name:

Proliferating Cell Nuclear Antigen Peptide 1

CPTC Name:

CPTC-PCNA Peptide 1

Aliases:

Cyclin; DNA Polymerase Delta Auxiliary Protein

Function:

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Chromosomal Localization:

20pter-p12

Accession Number:

NP_002583.1

UniProt Accession Number:

P12004

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

LVQGSILK

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

880

Last Updated:

06/11/2013

Links

Characterization Data

SOPs

No SOPs available.

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