Background
Catalog Number:
CPTC-PAK4-1
Target Antigen:
P21 (RAC1) Activated Kinase 4 Peptide 1
Isotype:
IgG
Species:
Rabbit Recombinant Cloned Antibody
Last Updated:
03/20/2024
Antigen Recognition(s):
Peptide, Recombinant Full-length, Endogenous, Phosphorylation
External Links
- CPTAC Assay Portal CPTAC-5891 (Phospho)
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (396.9 KB)
CPTC-PAK4-1 Evaluation by the Human Protein Atlas
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
IHC NCI60
Download Immuno-histochemistry of CPTC-PAK4-1 for NCI60 Cell Line Array with titer 1:50
0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green) (79.2 KB)
CPTC-PAK4-1 IHC NCI60
Result: Positive
Immuno-histochemistry of CPTC-PAK4-1 for NCI60 Cell Line Array with titer 1:50
0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green)
Characterization SOP Files
IHC Tissue
CPTC-PAK4-1 IHC Tissue
Result: Positive
Tissue Micro-Array(TMA) core of pancreatic cancer showing cell membrane staining using Antibody CPTC-PAK4-1. Titer: 1:50
Characterization SOP Files
Immuno-MRM
CPTC-PAK4-1 iMRM
Result: Positive
Immuno-MRM chromatogram of CPTC-PAK4-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5891)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysate.
Characterization SOP Files
Immunofluorescence
CPTC-PAK4-1
Result: Positive
Immunofluorescence staining of human cell line LCL57 with CPTC-PAK4-1 Ab shows localization to the cytoplasm.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-PAK4-1 western blot (recombinant protein)
Result: Presumed Positive (with additional bands)
Western Blot using CPTC-PAK4-1 as primary antibody against recombinant human p21 (Cdc42/Rac)-activated kinase 4 (PAK4), transcript variant 1 protein (lane 2). Also included are molecular weight standards (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-PAK4-1 western blot (cell lysates)
Result: Negative
Western Blot using CPTC-PAK4-1 as primary antibody against cell lysates LCL57 (lane 2), HeLa (lane 3) and MCF10A (lane 4). Also included are molecular weight standards (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-PAK4-1 automated western blot (cell lysates)
Result: Positive
Western Blot using CPTC-PAK4-1 as primary antibody against cell lysates LCL57 (lane 2), HeLa (lane 3) and MCF10A (lane 4). Also included are molecular weight standards (lane 1).
Characterization SOP Files
Background
NCI Identification Number:
00301
Antigen Name:
P21 (RAC1) Activated Kinase 4 Peptide 1
CPTC Name:
CPTC-PAK4 Peptide 1
Aliases:
P21 (RAC1) Activated Kinase 4; P21 Protein (Cdc42/Rac)-Activated Kinase 4; Serine/Threonine-Protein Kinase PAK 4; P21(CDKN1A)-Activated Kinase 4; EC 2.7.11.1; Protein Kinase Related To S. Cerevisiae STE20, Effector For Cdc42Hs; P21-Activated Kinase 4; EC 2.7.11; KIAA1142; PAK-4
Function:
PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.PAK4 (P21 (RAC1) Activated Kinase 4) is a Protein Coding gene. Diseases associated with PAK4 include Breast Adenoma. Among its related pathways are Immune response IL-23 signaling pathway and Human cytomegalovirus infection. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is PAK5.Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN.
Chromosomal Localization:
19q13.2
Accession Number:
NP_005875.1
UniProt Accession Number:
O96013
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
CRPLS(ph)GPDVGTPQPAGLASGAK
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
2420
Last Updated:
08/21/2020
Links
- EnsEMBL - EnsEMBL PAK4
- Entrez - Entrez PAK4
- Uniprot - Uniprot O96013
Characterization Data
SOPs
No SOPs available.
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