Background
Catalog Number:
CPTC-ODC1-1
RRID:
AB_1553747
Target Antigen:
Ornithine Decarboxylase 1
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
09/09/2021
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Purchase
- DSHB CPTC-ODC1-1
External Links
- Human Protein Atlas CPTC-ODC1-1
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (180.0 KB)
CPTC-ODC1-1 Evaluation by the Human Protein Atlas
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
IHC NCI60
CPTC-ODC1-1 IHC NCI60
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
IHC Tissue
CPTC-ODC1-1 IHC Tissue
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
Immunofluorescence - HPA
CPTC-ODC1-1 Evaluation by the Human Protein Atlas
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is partly supported by literature or no literature is available. Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
Human assay: A-431 fixed with PFA, dilution: 1:30
Human assay: U-2 OS fixed with PFA, dilution: 1:30
Human assay: U-251 MG fixed with PFA, dilution: 1:30
Characterization SOP Files
Indirect ELISA
CPTC-ODC1-1 Indirect ELISA
Result: Positive
Indirect ELISA of CPTC-ODC1-1
Characterization SOP Files
NCI 60 Protein Array
CPTC-ODC1-1 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - HPA - tissue or cell lysate
CPTC-ODC1-1 Evaluation by the Human Protein Atlas
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present. Analysis performed using a standard panel of samples. Antibody dilution: 1:500
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-ODC1-1 Western Blot
Result: Positive
Western Blot Using CPTC-ODC1-1 as primary Ab against Ag 10532(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).
Characterization SOP Files
Background
Catalog Number:
CPTC-ODC1-2
RRID:
AB_2267589
Target Antigen:
Ornithine Decarboxylase 1
Isotype:
IgG2a
Species:
Mouse Monoclonal Antibody
Last Updated:
05/28/2024
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Purchase
- DSHB CPTC-ODC1-2
Characterization Data [Compare Characterization Data]
CyTOF
CPTC-ODC1-2 Immuno-Mass Cytometry
Result: Positive
Imaging mass cytometry on bone marrow core using CPTC-ODC1-2 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (liver, bone marrow, spleen, colon, pancreas, breast, lung, endometrium, and kidney) and cancer tissues (breast, colon, ovarian, and lung).
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (272.9 KB)
CPTC-ODC1-2 Evaluation by the Human Protein Atlas
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
IHC NCI60
CPTC-ODC1-2 IHC NCI60
Result: Positive
Immuno-histochemistry of CPTC-ODC1-2 for NCI60 Cell Line Array at titer 1:500
0=NEGATIVE
1=WEAK (red)
2=MODERATE(blue)
3=STRONG(green)
Titer: 1:100
Characterization SOP Files
IHC Tissue
CPTC-ODC1-2 IHC Tissue
Result: Positive
Tissue Micro-Array(TMA) core of ovarian cancer showing cytoplasmic staining using Antibody CPTC-ODC1-2. Titer: 1:100
Characterization SOP Files
Indirect ELISA
CPTC-ODC1-2 Indirect ELISA
Result: Positive
Indirect ELISA of CPTC-ODC1-2
Characterization SOP Files
NCI 60 Protein Array
CPTC-ODC1-2 NCI60 Protein Array
Result: Positive
Protein Array in which CPTC-ODC1-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-ODC1-2 Western Blot
Result: Positive
Western Blot Using CPTC-ODC1-2 as primary Ab against Ag 10532(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).
Characterization SOP Files
Background
Catalog Number:
CPTC-ODC1-3
RRID:
AB_1553749
Target Antigen:
Ornithine Decarboxylase 1
Isotype:
IgG2a
Species:
Mouse Monoclonal Antibody
Last Updated:
05/28/2024
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Purchase
- DSHB CPTC-ODC1-3
Characterization Data [Compare Characterization Data]
Affinity Measurement by SPR
CPTC-ODC1-3 SPR
Result: Positive
Kinetic titration data for ODC1-3 Ab (615A3.1) using Biacore SPR method
Characterization SOP Files
CyTOF
CPTC-ODC1-3 Immuno-Mass Cytometry
Result: Positive
Imaging mass cytometry on breast cancer tissue core using CPTC-ODC1-3 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (liver, bone marrow, spleen, placenta, prostate, colon, pancreas, breast, lung, endometrium, appendix, and kidney) and cancer tissues (breast, colon, ovarian, lung, and prostate).
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (272.9 KB)
CPTC-ODC1-3 Evaluation by the Human Protein Atlas
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
IHC NCI60
CPTC-ODC1-3 IHC NCI60
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
IHC Tissue
CPTC-ODC1-3 IHC Tissue
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
Indirect ELISA
CPTC-ODC1-3 Indirect ELISA
Result: Positive
Indirect ELISA of CPTC-ODC1-3
Characterization SOP Files
NCI 60 Protein Array
CPTC-ODC1-3 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-ODC1-3 Western Blot
Result: Positive
Western Blot Using CPTC-ODC1-3 as primary Ab against Ag 10532(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).
Characterization SOP Files
Background
NCI Identification Number:
10532
Antigen Name:
Ornithine Decarboxylase 1
CPTC Name:
CPTC-ODC1
Aliases:
ODC1; EC 4.1.1.17; ODC
Function:
This gene encodes the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter.
Chromosomal Localization:
2p25
Expression System:
E. Coli
Accession Number:
BC025296
UniProt Accession Number:
P11926
DNA Source:
HIP:HsCD00005772
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
53205
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMNNFGNEEFDCHFLDEGFTAKDILDQKINEVSSSDDKDAFYVADLGD
ILKKHLRWLKALPRVTPFYAVKCNDSKAIVKTLAATGTGFDCASKTEIQL
VQSLGVPPERIIYANPCKQVSQIKYAANNGVQMMTFDSEVELMKVARAHP
KAKLVLRIATDDSKAVCRLSVKFGATLRTSRLLLERAKELNIDVVGVSFH
VGSGCTDPETFVQAISDARCVFDMGAEVGFSMYLLDIGGGFPGSEDVKLK
FEEITGVINPALDKYFPSDSGVRIIAEPGRYYVASAFTLAVNIIAKKIVL
KEQTGSDDEDESSEQTFMYYVNDGVYGSFNCILYDHAHVKPLLQKRPKPD
EKYYSSSIWGPTCDGLDRIVERCDLPEMHVGDWMLFENMGAYTVAAASTF
NGFQRPTIYYVMSGPAWQLMQQFQNPDFPPEVEEQDASTLPVSCAWESGM
KRHRAACASASINV
Native Sequence:
MNNFGNEEFDCHFLDEGFTAKDILDQKINEVSSSDDKDAFYVADLGDILK
KHLRWLKALPRVTPFYAVKCNDSKAIVKTLAATGTGFDCASKTEIQLVQS
LGVPPERIIYANPCKQVSQIKYAANNGVQMMTFDSEVELMKVARAHPKAK
LVLRIATDDSKAVCRLSVKFGATLRTSRLLLERAKELNIDVVGVSFHVGS
GCTDPETFVQAISDARCVFDMGAEVGFSMYLLDIGGGFPGSEDVKLKFEE
ITGVINPALDKYFPSDSGVRIIAEPGRYYVASAFTLAVNIIAKKIVLKEQ
TGSDDEDESSEQTFMYYVNDGVYGSFNCILYDHAHVKPLLQKRPKPDEKY
YSSSIWGPTCDGLDRIVERCDLPEMHVGDWMLFENMGAYTVAAASTFNGF
QRPTIYYVMSGPAWQLMQQFQNPDFPPEVEEQDASTLPVSCAWESGMKRH
RAACASASINV
Calculated Isoelectric Point:
5.1
Molecular Weight:
51420
Last Updated:
09/01/2008
Links
- EnsEMBL - EnsEMBL ODC1
- Entrez - Entrez ODC1
- Uniprot - Uniprot P11926
Characterization Data
Gel
Click image to enlarge
Ag 10532 Gel
PAGE of Ag 10532 with molecular weight standards
Characterization SOP Files
SOPs
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