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Neural precursor cell expressed, developmentally down-regulated 4 isoform 1

Antibody: CPTC-NEDD4-1
Background

Catalog Number:

CPTC-NEDD4-1

RRID:

AB_2617296

Target Antigen:

Neural precursor cell expressed, developmentally down-regulated 4 isoform 1

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

08/25/2021

Antigen Recognition(s):

Recombinant Full-length

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Characterization Data [Compare Characterization Data]
  IHC HPA

CPTC-NEDD4-1 Evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-NEDD4-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-NEDD4-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-NEDD4-1 as primary Ab against NEDD4 (rAg 00011) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-NEDD4-1 Western blot

Result: Positive

Western Blot using CPTC-NEDD4-1 as primary Ab against NEDD4 (rAg 00011) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antibody: CPTC-NEDD4-2
Background

Catalog Number:

CPTC-NEDD4-2

RRID:

AB_2617297

Target Antigen:

Neural precursor cell expressed, developmentally down-regulated 4 isoform 1

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

09/09/2021

Antigen Recognition(s):

Recombinant Full-length, Endogenous

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External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (211.5 KB)

CPTC-NEDD4-2 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immunofluorescence - HPA
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is partly supported by literature or no literature is available. Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Human assay: HeLa fixed with PFA, dilution: 1:9 Human assay: MCF7 fixed with PFA, dilution: 1:9 Human assay: U-2 OS fixed with PFA, dilution: 1:9 Click image to enlarge

CPTC-NEDD4-2 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is partly supported by literature or no literature is available. Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Human assay: HeLa fixed with PFA, dilution: 1:9
Human assay: MCF7 fixed with PFA, dilution: 1:9
Human assay: U-2 OS fixed with PFA, dilution: 1:9


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-NEDD4-2 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-NEDD4-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-NEDD4-2 as primary Ab against NEDD4 (rAg 00011) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-NEDD4-2 Western blot

Result: Positive

Western Blot using CPTC-NEDD4-2 as primary Ab against NEDD4 (rAg 00011) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antibody: CPTC-NEDD4-3
Background

Catalog Number:

CPTC-NEDD4-3

RRID:

AB_2617298

Target Antigen:

Neural precursor cell expressed, developmentally down-regulated 4 isoform 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

08/25/2021

Antigen Recognition(s):

Recombinant Full-length

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA

CPTC-NEDD4-3 Evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-NEDD4-3 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-NEDD4-3 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-NEDD4-3 as primary Ab against NEDD4 (rAg 00011) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-NEDD4-3 Western blot

Result: Positive

Western Blot using CPTC-NEDD4-3 as primary Ab against NEDD4 (rAg 00011) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antigen: Neural precursor cell expressed, developmentally down-regulated 4 isoform 1

Background

NCI Identification Number:

00011

Antigen Name:

Neural precursor cell expressed, developmentally down-regulated 4 isoform 1

CPTC Name:

CPTC-NEDD4

Aliases:

neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitinprotein ligase; NEDD4-1; RPF1; KIAA0093; Cell proliferation-inducing gene 53 protein; Neural precursor cell expressed developmentally down-regulated protein 4; NEDD-4; MGC176705; neural precursor cell expressed, developmentally down-regulated 4; E3 ubiquitin-protein ligase NEDD4; receptor-potentiating factor 1; EC 6.3.2.

Function:

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a
thioester and then directly transfers the ubiquitin to targeted substrates. Involved in the pathway leading to the
degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Ubiquitinates FGFR1, leading to receptor
internalization and degradation in lysosomes. According to PubMed:18562292 the direct link between NEDD4 and PTEN
regulation through polyubiquitination described in PubMed:17218260 is questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD. Involved in the budding of many viruses. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates
TNK2 and regulates EGF-induced degradation of EGFR and TNF2.

Chromosomal Localization:

15q21.3

Expression System:

E. coli

Accession Number:

NM_006154

UniProt Accession Number:

P46934

DNA Source:

Mammalian Gene Collection

Immunogen:

Recombinant Full Length Protein

Vector Name:

pET28-MHL

Extinction Coefficient:

Buffers:

PBS, pH 7.4

Expressed Sequence:

mhhhhhhssgrenlyfqgMATCAVEVFGLLEDEENSRIVRVRVIAGIGLA
KKDILGASDPYVRVTLYDPMNGVLTSVQTKTIKKSLNPKWNEEILFRVHP
QQHRLLFEVFDENRLTRDDFLGQVDVPLYPLPTENPRLERPYTFKDFVLH
PRSHKSRVKGYLRLKMTYLPKTSGSEDDNAEQAEELEPGWVVLDQPDAAC
HLQQQQEPSPLPPGWEERQDILGRTYYVNHESRRTQWKRPTPQDNLTDAE
NGNIQLQAQRAFTTRRQISEETESVDNQESSENWEIIREDEATMYSSQAF
PSPPPSSNLDVPTHLAEELNARLTIFGNSAVSQPASSSNHSSRRGSLQAY
TFEEQPTLPVLLPTSSGLPPGWEEKQDERGRSYYVDHNSRTTTWTKPTVQ
ATVETSQLTSSQSSAGPQSQASTSDSGQQVTQPSEIEQGFLPKGWEVRHA
PNGRPFFIDHNTKTTTWEDPRLKIPAHLRGKTSLDTSNDLGPLPPGWEER
THTDGRIFYINHNIKRTQWEDPRLENVAITGPAVPYSRDYKRKYEFFRRK
LKKQNDIPNKFEMKLRRATVLEDSYRRIMGVKRADFLKARLWIEFDGEKG
LDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHL
SYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLHKPITLHDMESVDSEYY
NSLRWILENDPTELDLRFIIDEELFGQTHQHELKNGGSEIVVTNKNKKEY
IYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGD
VDVNDWREHTKYKNGYSANHQVIQWFWKAVLMMDSEKRIRLLQFVTGTSR
VPMNGFAELYGSNGPQSFTVEQWGTPEKLPRAHTCFNRLDLPPYESFEEL
WDKLQMAIENTQG

Native Sequence:

Calculated Isoelectric Point:

5.9

Molecular Weight:

105822

Last Updated:

08/22/2020

Links

Characterization Data

Gel

Click to enlarge image PAGE of NEDD4 (Ag 00011) with molecular weight standards in lane 1
Click image to enlarge

CPTC-NEDD4 PAGE

PAGE of NEDD4 (Ag 00011) with molecular weight standards in lane 1

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SOPs

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