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Nuclear Receptor Co-Repressor 1

Antibody: CPTC-NCOR1-1
Background

Catalog Number:

CPTC-NCOR1-1

RRID:

AB_2617294

Target Antigen:

Nuclear Receptor Co-Repressor 1

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

08/25/2021

Antigen Recognition(s):

Recombinant Full-length

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA

CPTC-NCOR1-1 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-NCOR1-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-NCOR1-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-NCOR1-1 as primary Ab against NCOR1 (rAg 00009) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-NCOR1-1 Western blot

Result: Positive

Western Blot using CPTC-NCOR1-1 as primary Ab against NCOR1 (rAg 00009) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antibody: CPTC-NCOR1-2
Background

Catalog Number:

CPTC-NCOR1-2

RRID:

AB_2617295

Target Antigen:

Nuclear Receptor Co-Repressor 1

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

09/09/2021

Antigen Recognition(s):

Recombinant Full-length, Endogenous

SOP:

Purchase
External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (165.4 KB)

CPTC-NCOR1-2 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-NCOR1-2 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-NCOR1-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - HPA - tissue or cell lysate
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present. Analysis performed using a standard panel of samples. Antibody dilution: 1:500. Click image to enlarge

CPTC-NCOR1-2 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present. Analysis performed using a standard panel of samples. Antibody dilution: 1:500.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-NCOR1-2 as primary Ab against NCOR1 (rAg 00009) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-NCOR1-2 Western blot

Result: Positive

Western Blot using CPTC-NCOR1-2 as primary Ab against NCOR1 (rAg 00009) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antibody: CPTC-NCOR1-3
Background

Catalog Number:

CPTC-NCOR1-3

Target Antigen:

Nuclear Receptor Co-Repressor 1

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

08/25/2021

Antigen Recognition(s):

Recombinant Full-length

SOP:

Characterization Data [Compare Characterization Data]
  IHC HPA

CPTC-NCOR1-3 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-NCOR1-3 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-NCOR1-3 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-NCOR1-3 as primary Ab against NCOR1 (rAg 00009) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-NCOR1-3 Western blot

Result: Positive

Western Blot using CPTC-NCOR1-3 as primary Ab against NCOR1 (rAg 00009) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antigen: Nuclear Receptor Co-Repressor 1

Background

NCI Identification Number:

00009

Antigen Name:

Nuclear Receptor Co-Repressor 1

CPTC Name:

CPTC-NCOR1

Aliases:

nuclear receptor corepressor 1; N-CoR; TRAC1; hN-CoR; KIAA1047; N-CoR1; MGC104216; hCIT529I10; thyroid hormone- and retinoic acid receptor-associated corepressor 1

Function:

This gene encodes a protein that mediates ligand-independent transcription repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. It is part of a complex which also includes histone deacetylases and transcriptional regulators similar to the yeast
protein Sin3p. This gene is located between the Charcot-Marie-Tooth and Smith-Magenis syndrome critical regions on chromosome 17. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 17 and 20.

Mediates transcriptional repression by certain nuclear receptors. Part of a complex which promotes histone
deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription
factors

Chromosomal Localization:

17p11.2

Expression System:

E. coli

Accession Number:

NM_006311

UniProt Accession Number:

O75376

DNA Source:

Mammalian Gene Collection (MGC)

Immunogen:

Recombinant Full Length Protein

Vector Name:

pET28-MHL

Extinction Coefficient:

Buffers:

PBS pH 7.4

Expressed Sequence:

MHHHHHHSSGRENLYFQGTEPVETSRWTEEEMEVAKKGLVEHGRNWAAIA
KMVGTKSEAQCKNFYFNYKRRHNLDNLLQ

Native Sequence:

Calculated Isoelectric Point:

8.3

Molecular Weight:

9377

Last Updated:

08/22/2020

Links

Characterization Data

Gel

Click to enlarge image PAGE of NCOR1 (Ag 00009) with molecular weight standards in lane 1
Click image to enlarge

CPTC-NCOR1 PAGE

PAGE of NCOR1 (Ag 00009) with molecular weight standards in lane 1

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SOPs

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