PRINT

Neural Cell Adhesion Molecule 1 Peptide 1

Antibody: CPTC-NCAM1-1
Background

Catalog Number:

CPTC-NCAM1-1

Target Antigen:

Neural Cell Adhesion Molecule 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

07/03/2024

Antigen Recognition(s):

Peptide, Endogenous

External Links
Purchase
Characterization Data [Compare Characterization Data]
  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-NCAM1-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6212) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue Click image to enlarge

CPTC-NCAM1-1 iMRM (Frozen Tissue)

Result: Positive

Immuno-MRM chromatogram of CPTC-NCAM1-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6212)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue


Characterization SOP Files

  NCI 60 Protein Array

CPTC-NCAM1-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide

CPTC-NCAM1-1 Western Blot Recombinant Protein

Result: Negative

Western blot using CPTC-NCAM1-1 as primary antibody against human neural cell adhesion molecule 1 (NCAM1), transcript variant 2, recombinant protein (lane 2). Expected molecular weight - 94.4 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-NCAM1-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and NCI-H226 (lane 6) whole cell lysates. Expected molecular weight - 95.6 kDa, 93.4 kDa, 83.8 kDa, 80.3 kDa, 73.5 kDa, and 40.8 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-NCAM1-1 Western Blot Cell Lysate

Result: Negative

Western blot using CPTC-NCAM1-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and NCI-H226 (lane 6) whole cell lysates. Expected molecular weight - 95.6 kDa, 93.4 kDa, 83.8 kDa, 80.3 kDa, 73.5 kDa, and 40.8 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-NCAM1-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 95.6 kDa, 93.4 kDa, 83.8 kDa, 80.3 kDa, 73.5 kDa, and 40.8 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-NCAM1-1 Automated Western Blot Cell Lysate

Result: Negative

Automated western blot using CPTC-NCAM1-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 95.6 kDa, 93.4 kDa, 83.8 kDa, 80.3 kDa, 73.5 kDa, and 40.8 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

[top]

Antigen: Neural Cell Adhesion Molecule 1 Peptide 1
Background

NCI Identification Number:

00384

Antigen Name:

Neural Cell Adhesion Molecule 1 Peptide 1

CPTC Name:

CPTC-NCAM1 Peptide 1

Aliases:

Neural Cell Adhesion Molecule 1; NCAM; CD56; Antigen Recognized By Monoclonal Antibody 5.1H11; Neural Cell Adhesion Molecule, NCAM; CD56 Antigen; N-CAM-1; NCAM-1; MSK39; NCAM1

Function:

This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. NCAM1 (Neural Cell Adhesion Molecule 1) is a Protein Coding gene. Diseases associated with NCAM1 include Rabies and Bile Duct Cancer. Among its related pathways are Neuroscience and RET signaling. Gene Ontology (GO) annotations related to this gene include identical protein binding. An important paralog of this gene is NCAM2.This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. (Microbial infection) Acts as a receptor for rabies virus. Cell adhesion molecules (CAMs) are a large family of transmembrane proteins that are involved in the binding of a cell to another cell or to the extracellular matrix. They have roles in cell proliferation, differentiation, motility, trafficking, apoptosis and tissue architecture.

Chromosomal Localization:

11q23.2

Accession Number:

NP_000606.3

UniProt Accession Number:

P13591

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

GLGEISAASEFK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1320

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

[top]

Don't have Adobe Reader™?

Get it for free at Adobe.com

  [top]