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MutY DNA Glycosylase Peptide 1

Antibody: CPTC-MUTYH-1
Background

Catalog Number:

CPTC-MUTYH-1

Target Antigen:

MutY DNA Glycosylase Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

07/03/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

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Characterization Data [Compare Characterization Data]
  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-MUTYH-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6218 for oxidized peptide and https://assays.cancer.gov/CPTAC-6229 for not oxidized peptide) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue Click image to enlarge

CPTC-MUTYH-1 iMRM (Frozen Tissue)

Result: Positive

Immuno-MRM chromatogram of CPTC-MUTYH-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6218 for oxidized peptide and https://assays.cancer.gov/CPTAC-6229 for not oxidized peptide)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue


Characterization SOP Files

  NCI 60 Protein Array

CPTC-MUTYH-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-MUTYH-1 as primary antibody against human mutY homolog (E. coli) (MUTYH / MYH), transcript variant alpha1, full length, with N-terminal HIS tag, recombinant protein expressed in E. coli (lane 2). Expected molecular weight - 59.9 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-MUTYH-1 Western Blot Recombinant Protein

Result: Positive

Western blot using CPTC-MUTYH-1 as primary antibody against human mutY homolog (E. coli) (MUTYH / MYH), transcript variant alpha1, full length, with N-terminal HIS tag, recombinant protein expressed in E. coli (lane 2). Expected molecular weight - 59.9 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-MUTYH-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and NCI-H226 (lane 6) whole cell lysates. Expected molecular weight - 60.1 kDa, 59.1 kDa, 58.4 kDa, 57.5 kDa, and 57.4 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-MUTYH-1 Western Blot Cell Lysate

Result: Negative

Western blot using CPTC-MUTYH-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and NCI-H226 (lane 6) whole cell lysates. Expected molecular weight - 60.1 kDa, 59.1 kDa, 58.4 kDa, 57.5 kDa, and 57.4 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-MUTYH-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 60.1 kDa, 59.1 kDa, 58.4 kDa, 57.5 kDa, and 57.4 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-MUTYH-1 Automated Western Blot Cell Lysate

Result: Negative

Automated western blot using CPTC-MUTYH-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 60.1 kDa, 59.1 kDa, 58.4 kDa, 57.5 kDa, and 57.4 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antigen: MutY DNA Glycosylase Peptide 1
Background

NCI Identification Number:

00383

Antigen Name:

MutY DNA Glycosylase Peptide 1

CPTC Name:

CPTC-MUTYH Peptide 1

Aliases:

MutY DNA Glycosylase; MYH; Adenine DNA Glycosylase; MutY Homolog; A/G-Specific Adenine DNA Glycosylase; MutY Homolog (E. Coli); MutY-Like Protein; EC 3.2.2.31; EC 3.2.2; MUTYH; HMYH

Function:

This gene encodes a DNA glycosylase involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. This gene product is thought to play a role in signaling apoptosis by the introduction of single-strand breaks following oxidative damage. Mutations in this gene result in heritable predisposition to colorectal cancer, termed MUTYH-associated polyposis (MAP). Multiple transcript variants encoding different isoforms have been found for this gene.MUTYH (MutY DNA Glycosylase) is a Protein Coding gene. Diseases associated with MUTYH include Familial Adenomatous Polyposis 2 and Gastric Cancer. Among its related pathways are Telomere C-strand (Lagging Strand) Synthesis and Depurination. Gene Ontology (GO) annotations related to this gene include hydrolase activity and hydrolase activity, acting on glycosyl bonds. An important paralog of this gene is ENSG00000288208.Involved in oxidative DNA damage repair. Initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities.

Chromosomal Localization:

1p34.1

Accession Number:

NP_036354.1

UniProt Accession Number:

Q9UIF7

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

MGQQVLDNFFR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1210

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

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