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Mesothelin Peptide 2

Antibody: CPTC-MSLN-2
Background

Catalog Number:

CPTC-MSLN-2

Target Antigen:

Mesothelin Peptide 2

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

07/03/2024

Antigen Recognition(s):

Peptide, Endogenous

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (499.4 KB)

CPTC-MSLN-2 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  IHC Tissue

CPTC-MSLN-2 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-MSLN-2 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6242) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue Click image to enlarge

CPTC-MSLN-2 iMRM (Frozen Tissue)

Result: Positive

Immuno-MRM chromatogram of CPTC-MSLN-2 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6242)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue


Characterization SOP Files

  Immunofluorescence - HPA
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is partly supported by literature or no literature is available. Immunofluorescent staining of human cell line THP-1 shows localization to plasma membrane. Human assay: THP-1 fixed with PFA, dilution: 1:200 Human assay: U2OS fixed with PFA, dilution: 1:200 Click image to enlarge

CPTC-MSLN-2 evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is partly supported by literature or no literature is available. Immunofluorescent staining of human cell line THP-1 shows localization to plasma membrane.


Human assay: THP-1 fixed with PFA, dilution: 1:200
Human assay: U2OS fixed with PFA, dilution: 1:200


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-MSLN-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-MSLN-2 NCI 60 Protein Array

Result: Positive

Protein Array in which CPTC-MSLN-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate
Click to enlarge image Western blot using CPTC-MSLN-2 as primary antibody against of mesothelin (MSLN), transcript variant 1 transient overexpression lysate. The expected molecular weight is 67.9 kDa. Click image to enlarge

CPTC-MSLN-2 Western Blot (Over-Expressed Lysate)

Result: Presumed Positive (with additional bands)

Western blot using CPTC-MSLN-2 as primary antibody against of mesothelin (MSLN), transcript variant 1 transient overexpression lysate. The expected molecular weight is 67.9 kDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-MSLN-2 Western Blot (Cell Lysate)

Result: Negative

Western blot using CPTC-MSLN-2 as primary antibody against human PBMC (2), HeLa (3), Jurkat (4), A549 (5), MCF7 (6) and H226 (7) whole cell lysates. The expected molecular weight is 69.0 kDa. Cytochrome C was used as a loading control.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-MSLN-2 Simple Western (cell lysates)

Result: Negative

Automated WB (Simple Western) using CPTC-MSNL-2 as primary antibody against the whole lysate of PBMC, HeLa, Jurkat, A549, MCF7 and H226. Expected MW is 69 KDa. The antibody does not recognize the target in Jurkat, A549 and H226 lysates. The same cell lysates were probed with an anti-CytC antibody.


Characterization SOP Files

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Antigen: Mesothelin Peptide 2
Background

NCI Identification Number:

00440

Antigen Name:

Mesothelin Peptide 2

CPTC Name:

CPTC-MSLN Peptide 2

Aliases:

Mesothelin; MPF; Pre-Pro-Megakaryocyte-Potentiating Factor; CAK1 Antigen; Soluble MPF Mesothelin Related Protein; Megakaryocyte Potentiating Factor; SMRP; MSLN; CAK1

Function:

This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.MSLN (Mesothelin) is a Protein Coding gene. Diseases associated with MSLN include Mesothelioma, Malignant and Asbestosis. Among its related pathways are Metabolism of proteins and Adhesion. An important paralog of this gene is MSLNL.

Chromosomal Localization:

16p13.3

Accession Number:

NP_005814.2

UniProt Accession Number:

Q13421

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

EIDESLIFYK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1100

Last Updated:

09/29/2020

Links

Characterization Data

SOPs

No SOPs available.

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