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MutS Homolog 3 Peptide 1

Antibody: CPTC-MSH3-1
Background

Catalog Number:

CPTC-MSH3-1

Target Antigen:

MutS Homolog 3 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

10/04/2024

Antigen Recognition(s):

Peptide, Endogenous

Thematic Panel(s):

Immuno-Oncology (IO)

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Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry
Click to enlarge image Affinity and binding kinetics of CPTC-MSH3-1 and BSA-conjugated peptide, "CDLSDFPLIK", were measured using biolayer interferometry. BSA-conjugated peptide was amine coupled over AR2G bio- sensors. Rabbit antibody CPTC-MSH3-1 was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, and 4 nM. Buffer only and biosensors immobilized without peptide were used as references for background subtraction. All binding data were double referenced and analyzed globally using a 1:2 bivalent fitting model. Click image to enlarge

CPTC-MSH3-1 Affinity and Kinetics (Bio-Layer Interferometry)

Result: Positive

Affinity and binding kinetics of CPTC-MSH3-1 and BSA-conjugated peptide, "CDLSDFPLIK", were measured using biolayer interferometry. BSA-conjugated peptide was amine coupled over AR2G bio- sensors. Rabbit antibody CPTC-MSH3-1 was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, and 4 nM. Buffer only and biosensors immobilized without peptide were used as references for background subtraction. All binding data were double referenced and analyzed globally using a 1:2 bivalent fitting model.


Characterization SOP Files

  Affinity Measurement by SPR
Click to enlarge image Affinity and binding kinetics of CPTC-MSH3-1 and BSA-conjugated peptide, "CDLSDFPLIK" were measured using surface plasmon resonance. BSA-conjugated peptide was amine coupled onto a Series S CM5 biosensor chip. CPTC-MSH3-1 rabbit antibody was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, and 0.25 nM. All binding data were double referenced and analyzed globally using a 1:2 bivalent fitting model. Click image to enlarge

CPTC-MSH3-1 Affinity and Kinetics (Surface Plasmon Resonance)

Result: Positive

Affinity and binding kinetics of CPTC-MSH3-1 and BSA-conjugated peptide, "CDLSDFPLIK" were measured using surface plasmon resonance. BSA-conjugated peptide was amine coupled onto a Series S CM5 biosensor chip. CPTC-MSH3-1 rabbit antibody was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, and 0.25 nM. All binding data were double referenced and analyzed globally using a 1:2 bivalent fitting model.


Characterization SOP Files

  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (487.7 KB)

CPTC-MSH3-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  IHC Tissue

CPTC-MSH3-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-MSH3-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6246) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue Click image to enlarge

CPTC-MSH3-1 iMRM (Frozen Tissue)

Result: Positive

Immuno-MRM chromatogram of CPTC-MSH3-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6246)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (487.7 KB)

CPTC-MSH3-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-MSH3-1 as primary rabbit antibody against BSA-conjugated peptide, CDLSDFPLIK, coated on the plate and detected using goat anti-rabbit antibody and TMB. Click image to enlarge

CPTC-MSH3-1 Indirect ELISA

Result: Positive

Indirect ELISA using CPTC-MSH3-1 as primary rabbit antibody against BSA-conjugated peptide, CDLSDFPLIK, coated on the plate and detected using goat anti-rabbit antibody and TMB.


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-MSH3-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-MSH3-1 NCI 60 Protein Array

Result: Presumed Positive

Protein Array in which CPTC-MSH3-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate

CPTC-MSH3-1 Western Blot (Over-Expressed Lysate)

Result: Negative

Western blot using CPTC-MSH3-1 as primary antibody against mutS homolog 3 (E. coli) (MSH3) transient overexpression lysate. The expected molecular weight is 127.2 kDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-MSH3-1 Western Blot (Cell Lysate)

Result: Negative

Western blot using CPTC-MSH3-1 as primary antibody against human PBMC (2), HeLa (3), Jurkat (4), A549 (5), MCF7 (6) and H226 (7) whole cell lysates. The expected molecular weight is 127.4 kDa. Cytochrome C was used as a loading control.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-MSH3-1 Simple Western (cell lysates)

Result: Negative

Automated WB (Simple Western) using CPTC-MSH3-1 as primary antibody against the whole lysate of PBMC, HeLa, Jurkat, A549, MCF7 and H226. Expected MW is 127.4 KDa. The antibody recognizes the target in Jurkat, A549 adn H226 lysates. The same cell lysates were probed with an anti-CytC antibody.


Characterization SOP Files

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Antigen: MutS Homolog 3 Peptide 1
Background

NCI Identification Number:

00501

Antigen Name:

MutS Homolog 3 Peptide 1

CPTC Name:

CPTC-MSH3 Peptide 1

Aliases:

MutS Homolog 3; MRP1; DUP; Divergent Upstream Protein; Mismatch Repair Protein 1; DNA Mismatch Repair Protein Msh3; HMSH3; Epididymis Secretory Sperm Binding Protein; MutS (E. Coli) Homolog 3; MutS Homolog 3 (E. Coli); FAP4; DUC1; DUG

Function:

The protein encoded by this gene forms a heterodimer with MSH2 to form MutS beta, part of the post-replicative DNA mismatch repair system. MutS beta initiates mismatch repair by binding to a mismatch and then forming a complex with MutL alpha heterodimer. This gene contains a polymorphic 9 bp tandem repeat sequence in the first exon. The repeat is present 6 times in the reference genome sequence and 3-7 repeats have been reported. Defects in this gene are a cause of susceptibility to endometrial cancer.MSH3 (MutS Homolog 3) is a Protein Coding gene. Diseases associated with MSH3 include Familial Adenomatous Polyposis 4 and Endometrial Cancer. Among its related pathways are Mismatch repair and Colorectal Cancer Metastasis. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and single-stranded DNA binding. An important paralog of this gene is MSH6.Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.

Chromosomal Localization:

5q14.1

Accession Number:

NP_002430.3

UniProt Accession Number:

P20585

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

DLSDFPLIK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

990

Last Updated:

10/12/2021

Links

Characterization Data

SOPs

No SOPs available.

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