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MutS Homolog 2 Peptide 1

Antibody: CPTC-MSH2-1
Background

Catalog Number:

CPTC-MSH2-1

Target Antigen:

MutS Homolog 2 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

07/03/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (277.2 KB)

CPTC-MSH2-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of colon cancer showing nuclear staining using Antibody CPTC-MSH2-1. Titer: 1:50 Click image to enlarge

CPTC-MSH2-1 IHC Tissue

Result: Positive

Tissue Micro-Array (TMA) core of colon cancer showing nuclear staining using Antibody CPTC-MSH2-1. Titer: 1:50


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-MSH2-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6240) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue Click image to enlarge

CPTC-MSH2-1 iMRM (Frozen Tissue)

Result: Positive

Immuno-MRM chromatogram of CPTC-MSH2-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6240)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue


Characterization SOP Files

  NCI 60 Protein Array

CTPC-MSH2-1 NCI60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-MSH2-1 as primary antibody against human mutS homolog 2, colon cancer, nonpolyposis type 1 (MSH2) recombinant protein (lane 2). Expected molecular weight - 104.6 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-MSH2-1 Western Blot (recombinant protein)

Result: Positive

Western blot using CPTC-MSH2-1 as primary antibody against human mutS homolog 2, colon cancer, nonpolyposis type 1 (MSH2) recombinant protein (lane 2). Expected molecular weight - 104.6 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-MSH2-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) cell lysates. Expected molecular weight - 105 kDa. Molecular weight standards are also included (lane 1). Data is negative/inconclusive. Click image to enlarge

CPTC-MSH2-1 Automated Western Blot (Cell Lysate)

Result: Negative

Automated western blot using CPTC-MSH2-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) cell lysates. Expected molecular weight - 105 kDa. Molecular weight standards are also included (lane 1). Data is negative/inconclusive.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-MSH2-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5) and NCI H226 (lane 6) cell lysates. Expected molecular weight 105 kDa. Molecular weight standards (MW Stds.) are also included (lane 1). Positive for Cell lines HeLa, MCF7 and NCI H226. Negative/inconsistent results for the other cell lines. Click image to enlarge

CPTC-MSH2-1 Western Blot (Cell Lysate)

Result: Presumed Positive (with additional bands)

Western blot using CPTC-MSH2-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5) and NCI H226 (lane 6) cell lysates. Expected molecular weight 105 kDa. Molecular weight standards (MW Stds.) are also included (lane 1). Positive for Cell lines HeLa, MCF7 and NCI H226. Negative/inconsistent results for the other cell lines.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-MSH2-1 as primary antibody against human lung (2), spleen (3), endometrium (4), breast (5), and ovary (6) tissue lysates. The expected molecular weight is 104.7 kDa and 97.3 kDa. Vinculin was used as a loading control. Click image to enlarge

CPTC-MSH2-1 Western Blot (Tissue Lysate)

Result: Negative

Western blot using CPTC-MSH2-1 as primary antibody against human lung (2), spleen (3), endometrium (4), breast (5), and ovary (6) tissue lysates. The expected molecular weight is 104.7 kDa and 97.3 kDa. Vinculin was used as a loading control.


Characterization SOP Files

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Antigen: MutS Homolog 2 Peptide 1
Background

NCI Identification Number:

00381

Antigen Name:

MutS Homolog 2 Peptide 1

CPTC Name:

CPTC-MSH2 Peptide 1

Aliases:

MutS Homolog 2; DNA Mismatch Repair Protein Msh2; HNPCC1; HNPCC; HMSH2; MutS (E. Coli) Homolog 2 (Colon Cancer, Nonpolyposis Type 1); MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli); MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1; DNA Mismatch Repair Protein Msh2 Transcript; MutS Protein Homolog 2; COCA1; LCFS2; FCC1; MSH2

Function:

This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene.MSH2 (MutS Homolog 2) is a Protein Coding gene. Diseases associated with MSH2 include Muir-Torre Syndrome and Lynch Syndrome I. Among its related pathways are Mismatch repair and Platinum Pathway, Pharmacokinetics/Pharmacodynamics. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and enzyme binding. An important paralog of this gene is MSH6.Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Recruits DNA helicase MCM9 to chromatin which unwinds the mismatch containing DNA strand (PubMed:26300262). ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.

Chromosomal Localization:

2p21-p16.3

Accession Number:

NP_000242.1

UniProt Accession Number:

P43246

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

LYQGINQLPNVIQALEK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1870

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

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