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Membrane Spanning 4-Domains A1 Peptide 1

Antibody: CPTC-MS4A1-1
Background

Catalog Number:

CPTC-MS4A1-1

Target Antigen:

Membrane Spanning 4-Domains A1 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

07/03/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

External Links
Characterization Data [Compare Characterization Data]
  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-MS4A1-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6239) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue Click image to enlarge

CPTC-MS4A1-1 iMRM (Frozen Tissue)

Result: Positive

Immuno-MRM chromatogram of CPTC-MS4A1-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6239)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue


Characterization SOP Files

  NCI 60 Protein Array

CPTC-MS4A1-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-MS4A1-1 as primary antibody against human membrane-spanning 4-domains, subfamily A, member 1 (MS4A1), transcript variant 3, recombinant protein (lane 2). Expected molecular weight - 32.9 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-MS4A1-1 Western Blot Recombinant Protein

Result: Presumed Positive (with additional bands)

Western blot using CPTC-MS4A1-1 as primary antibody against human membrane-spanning 4-domains, subfamily A, member 1 (MS4A1), transcript variant 3, recombinant protein (lane 2). Expected molecular weight - 32.9 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-MS4A1-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and NCI-H226 (lane 6) whole cell lysates. Expected molecular weight - 33.1 kDa and 14.6 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-MS4A1-1 Western Blot Cell Lysate

Result: Negative

Western blot using CPTC-MS4A1-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and NCI-H226 (lane 6) whole cell lysates. Expected molecular weight - 33.1 kDa and 14.6 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-MS4A1-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 33.1 kDa and 14.6 kDa. Molecular weight standards are also included (lane 1). Buffy coat is positive. All of the other cell lines are negative. Click image to enlarge

CPTC-MS4A1-1 Automated Western Blot Cell Lysate

Result: Negative

Automated western blot using CPTC-MS4A1-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 33.1 kDa and 14.6 kDa. Molecular weight standards are also included (lane 1). Buffy coat is positive. All of the other cell lines are negative.


Characterization SOP Files

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Antigen: Membrane Spanning 4-Domains A1 Peptide 1
Background

NCI Identification Number:

00380

Antigen Name:

Membrane Spanning 4-Domains A1 Peptide 1

CPTC Name:

CPTC-MS4A1 Peptide 1

Aliases:

Membrane Spanning 4-Domains A1; Bp35; Membrane-Spanning 4-Domains, Subfamily A, Member 1; Leukocyte Surface Antigen Leu-16; B-Lymphocyte Antigen CD20; CD20 Antigen; MS4A2; CD20; B1; Membrane-Spanning 4-Domains Subfamily A Member 1; B-Lymphocyte Cell-Surface Antigen B1; B-Lymphocyte Surface Antigen B1; CD20 Receptor; LEU-16; CVID5; MS4A1; S7

Function:

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein.MS4A1 (Membrane Spanning 4-Domains A1) is a Protein Coding gene. Diseases associated with MS4A1 include Immunodeficiency, Common Variable, 5 and Common Variable Immunodeficiency. Among its related pathways are Hematopoietic cell lineage and Hematopoietic Stem Cells and Lineage-specific Markers. Gene Ontology (GO) annotations related to this gene include epidermal growth factor receptor binding and MHC class II protein complex binding. An important paralog of this gene is MS4A8.B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:3925015, PubMed:7684739, PubMed:12920111). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:7684739, PubMed:12920111, PubMed:18474602).

Chromosomal Localization:

11q12.2

Accession Number:

NP_690605.1

UniProt Accession Number:

P11836

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

EEVVGLTETSSQPK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1540

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

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