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MRE11 Homolog, Double Strand Break Repair Nuclease Peptide 1

Antibody: CPTC-MRE11A-1
Background

Catalog Number:

CPTC-MRE11A-1

Target Antigen:

MRE11 Homolog, Double Strand Break Repair Nuclease Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Endogenous, Phosphorylation

External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (340.2 KB)

CPTC-MRE11A-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC NCI60
Download Immuno-histochemistry of CPTC-MRE11A-1 for NCI60 Cell Line Array with titer 1:250 0=NEGATIVE 1=WEAK(red) 2=MODERATE(blue) 3=STRONG(green) (54.4 KB)

CPTC-MRE11A-1 IHC NCI60

Result: Positive

Immuno-histochemistry of CPTC-MRE11A-1 for NCI60 Cell Line Array with titer 1:250
0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green)


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array(TMA) core of pancreatic cancer showing cytoplasmic staining using Antibody CPTC-MRE11A-1. Titer: 1:250 Click image to enlarge

CPTC-MRE11A-1 IHC Tissue

Result: Positive

Tissue Micro-Array(TMA) core of pancreatic cancer showing cytoplasmic staining using Antibody CPTC-MRE11A-1. Titer: 1:250


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-MRE11A-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5911) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysate. Click image to enlarge

CPTC-MRE11A-1 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-MRE11A-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5911)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysate.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-MRE11A-1 as primary antibody against cell lysates HeLa, MCF10A, and LCL57. Samples from each cell line were irradiated with 10 Gy as shown in the ‘+’ indicated lanes. Samples from each non-irradiated cell line were treated with alkaline phosphatase enzyme as shown in the ‘-‘ indicated lanes. Molecular weight standards are also included for each cell line. Click image to enlarge

CPTC-MRE11A-1 automated western blot (cell lysate)

Result: Negative

Automated western blot using CPTC-MRE11A-1 as primary antibody against cell lysates HeLa, MCF10A, and LCL57. Samples from each cell line were irradiated with 10 Gy as shown in the ‘+’ indicated lanes. Samples from each non-irradiated cell line were treated with alkaline phosphatase enzyme as shown in the ‘-‘ indicated lanes. Molecular weight standards are also included for each cell line.


Characterization SOP Files

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Antigen: MRE11 Homolog, Double Strand Break Repair Nuclease Peptide 1
Background

NCI Identification Number:

00316

Antigen Name:

MRE11 Homolog, Double Strand Break Repair Nuclease Peptide 1

CPTC Name:

CPTC-MRE11A Peptide 1

Aliases:

MRE11 Homolog, Double Strand Break Repair Nuclease; MRE11A; ATLD; MRE11 Homolog A, Double Strand Break Repair Nuclease; Double-Strand Break Repair Protein MRE11A; Double-Strand Break Repair Protein MRE11; Meiotic Recombination 11 Homolog 1; Meiotic Recombination 11 Homolog A; AT-Like Disease; MRE11 Homolog 1; HNGS1; MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae); MRE11 Homolog, Double Strand Break Repair Nuclease A; MRE11 Meiotic Recombination 11-Like Protein A; MRE11 Double Strand Break Repair Nuclease A; MRE11 Meiotic Recombination 11 Homolog A; DNA Recombination And Repair Protein; Endo/Exonuclease Mre11; MRE11 Homolog A; EC 3.1.-.-; MRE11B

Function:

This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms.MRE11 (MRE11 Homolog, Double Strand Break Repair Nuclease) is a Protein Coding gene. Diseases associated with MRE11 include Ataxia-Telangiectasia-Like Disorder 1 and Ataxia, Early-Onset, With Oculomotor Apraxia And Hypoalbuminemia. Among its related pathways are Cell Cycle Checkpoints and DNA Damage.Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547, PubMed:29670289). The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11 (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547, PubMed:29670289). RAD50 may be required to bind DNA ends and hold them in close proximity (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547, PubMed:29670289). This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point (PubMed:9651580, PubMed:9590181, PubMed:9705271, PubMed:11741547, PubMed:29670289, PubMed:30612738). The complex may also be required for DNA damage signaling via activation of the ATM kinase (PubMed:15064416). In telomeres the MRN complex may modulate t-loop formation.

Chromosomal Localization:

11q21

Accession Number:

NP_005582.1

UniProt Accession Number:

P49959

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

CIMS(ph)QSQVSK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1100

Last Updated:

08/31/2021

Links

Characterization Data

SOPs

No SOPs available.

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