Catalog Number:
CPTC-MKI67-2
RRID:
AB_2820269
Target Antigen:
Marker Of Proliferation Ki-67 Peptide 2
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
07/03/2024
Antigen Recognition(s):
Peptide, Recombinant Full-length, Endogenous
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Positive
Immuno-MRM chromatogram of CPTC-MKI67-2 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6247)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Result: Presumed Positive (with additional bands)
Western blot using CPTC-MKI67-2 as primary antibody against human antigen Ki-67 (MKI67), transcript variant 1, residues 1-1000 aa recombinant protein (lane 2). Expected molecular weight - 111 kDa. Molecular weight standards are also included (lane 1).
Result: Negative
Western blot using CPTC-MKI67-2 as primary antibody against HeLa (lane 1), Jurkat (lane 2), A549 (lane 3), MCF7 (lane 4), H226 (lane 5), and PBMC (lane 6) whole cell lysates. Expected molecular weight > 250 kDa. Molecular weight standards are also included.
Result: Negative
Western blot using CPTC-MKI67-2 as primary antibody against human lung (2), spleen (3), endometrium (4), breast (5), and ovary (6) tissue lysates. The expected molecular weight is 358.7 kDa and 319.4 kDa. Vinculin was used as a loading control.
Result: Negative
Automated WB (Simple Western) using CPTC-MKI67-2 as primary antibody against the whole lysate of PBMC, HeLa, Jurkat, A549, MCF7 and H226. Expected MW is 34.2 KDa. The antibody recognizes the target in Jurkat, A549 adn H226 lysates. The same cell lysates were probed with an anti-CytC antibody.
NCI Identification Number:
00246
Antigen Name:
Marker Of Proliferation Ki-67 Peptide 2
CPTC Name:
CPTC-MKI67 Peptide 2
Aliases:
Marker Of Proliferation Ki-67; Antigen Identified By Monoclonal Antibody Ki-67; Protein Phosphatase 1, Regulatory Subunit 105; Antigen KI-67; Antigen Ki67; Proliferation-Related Ki-67 Antigen; Proliferation Marker Protein Ki-67; PPP1R105; MIB-1; MIB-; KIA
Function:
This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. MKI67 (Marker Of Proliferation Ki-67) is a Protein Coding gene. Diseases associated with MKI67 include Cervical Intraepithelial Neoplasia and Bowen's Disease. Among its related pathways are Neuroscience and DNA Damage. Gene Ontology (GO) annotations related to this gene include protein C-terminus binding. An important paralog of this gene is CDCA2.Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
Chromosomal Localization:
10q26.2
Accession Number:
NP_002408.2
UniProt Accession Number:
P46013
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
LDQPGNLPGSNR
Native Sequence:
Calculated Isoelectric Point:
0
Molecular Weight:
1320
Last Updated:
10/22/2019
No SOPs available.
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