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Marker Of Proliferation Ki-67 Peptide 1

Antibody: CPTC-MKI67-1
Background

Catalog Number:

CPTC-MKI67-1

RRID:

AB_2820268

Target Antigen:

Marker Of Proliferation Ki-67 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

07/03/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (243.6 KB)

CPTC-MKI67-1 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of colon cancer showing nuclear staining using Antibody CPTC-MKI67-1. Titer: 1:500 Click image to enlarge

CPTC-MKI67-1 IHC Tissue

Result: Positive

Tissue Micro-Array (TMA) core of colon cancer showing nuclear staining using Antibody CPTC-MKI67-1. Titer: 1:500


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-MKI67-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6222) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue Click image to enlarge

CPTC-MKI67-1 iMRM (Frozen Tissue)

Result: Positive

Immuno-MRM chromatogram of CPTC-MKI67-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6222)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue


Characterization SOP Files

  Immunofluorescence
Click to enlarge image Immunofluorescence staining of HeLa cells using CPTC-MKI67-1 antibody (green). MKI67 protein expression shows localization to the nucleoplasm. Click image to enlarge

CPTC-MKI67-1 Immunofluorescence

Result: Positive

Immunofluorescence staining of HeLa cells using CPTC-MKI67-1 antibody (green). MKI67 protein expression shows localization to the nucleoplasm.


Characterization SOP Files

  Immunofluorescence - HPA
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line THP-1 shows localization to nucleoli rim & mitotic chromosome. Human assay: THP-1 fixed with PFA, dilution: 1:200 Human assay: U2OS fixed with PFA, dilution: 1:200 Click image to enlarge

CPTC-MKI67-1 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line THP-1 shows localization to nucleoli rim & mitotic chromosome.
Human assay: THP-1 fixed with PFA, dilution: 1:200
Human assay: U2OS fixed with PFA, dilution: 1:200


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-MKI67-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-MKI67-1 NCI 60 Protein Array

Result: Positive

Protein Array in which CPTC-MKI67-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-MKI67-1 as primary antibody against human antigen Ki-67 (MKI67), transcript variant 1, residues 1-1000 aa recombinant protein. Expected molecular weight - 111 kDa. Molecular weight standards are also included. Click image to enlarge

CPTC-MKI67-1 Western Blot (Recombinant Protein)

Result: Negative

Western blot using CPTC-MKI67-1 as primary antibody against human antigen Ki-67 (MKI67),
transcript variant 1, residues 1-1000 aa recombinant protein. Expected molecular weight - 111 kDa. Molecular weight standards are also included.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-MKI67-1 as primary antibody against HeLa (lane 1), Jurkat (lane 2), A549 (lane 3), MCF7 (lane 4), H226 (lane 5) and PBMC (lane 6) whole cell lysates. Expected molecular weight is > 250 kDa. Molecular weight standards are also included. Click image to enlarge

CPTC-MKI67-1 Western Blot (Cell Lysates)

Result: Negative

Western blot using CPTC-MKI67-1 as primary antibody against HeLa (lane 1), Jurkat (lane 2), A549 (lane 3), MCF7 (lane 4), H226 (lane 5) and PBMC (lane 6) whole cell lysates. Expected molecular weight is > 250 kDa. Molecular weight standards are also included.


Characterization SOP Files

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Antigen: Marker Of Proliferation Ki-67 Peptide 1
Background

NCI Identification Number:

00245

Antigen Name:

Marker Of Proliferation Ki-67 Peptide 1

CPTC Name:

CPTC-MKI67 Peptide 1

Aliases:

Marker Of Proliferation Ki-67; Antigen Identified By Monoclonal Antibody Ki-67; Protein Phosphatase 1, Regulatory Subunit 105; Antigen KI-67; Antigen Ki67; Proliferation-Related Ki-67 Antigen; Proliferation Marker Protein Ki-67; PPP1R105; MIB-1; MIB-; KIA

Function:

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. MKI67 (Marker Of Proliferation Ki-67) is a Protein Coding gene. Diseases associated with MKI67 include Cervical Intraepithelial Neoplasia and Bowen's Disease. Among its related pathways are Neuroscience and DNA Damage. Gene Ontology (GO) annotations related to this gene include protein C-terminus binding. An important paralog of this gene is CDCA2.Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).

Chromosomal Localization:

10q26.2

Accession Number:

NP_002408.2

UniProt Accession Number:

P46013

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

EEAQALEDLTGFK and EEAQSLEDLAGFK

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1430

Last Updated:

10/22/2019

Links

Characterization Data

SOPs

No SOPs available.

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