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Malate Dehydrogenase 1 NAD (soluble)

Antibody: CPTC-MDH1-1
Background

Catalog Number:

CPTC-MDH1-1

RRID:

AB_10805146

Target Antigen:

Malate Dehydrogenase 1 NAD (soluble)

Isotype:

IgG2c

Species:

Mouse Monoclonal Antibody

Last Updated:

05/28/2024

Antigen Recognition(s):

Recombinant Full-length, Endogenous

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External Links
Characterization Data [Compare Characterization Data]
  CyTOF
Click to enlarge image Imaging mass cytometry on breast cancer tissue core using CPTC-MBD1-1 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (liver, bone marrow, spleen, prostate, colon, pancreas, breast, lung, testis, endometrium, and appendix) and cancer tissues (breast, prostate, colon, ovarian, and lung). Click image to enlarge

CPTC-MDH1-1 Immuno-Mass Cytometry

Result: Positive

Imaging mass cytometry on breast cancer tissue core using CPTC-MBD1-1 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (liver, bone marrow, spleen, prostate, colon, pancreas, breast, lung, testis, endometrium, and appendix) and cancer tissues (breast, prostate, colon, ovarian, and lung).


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (100.9 KB)

CPTC-MDH1-1

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC NCI60

CPTC-MDH1-1 IHC NCI60

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IHC Tissue

CPTC-MDH1-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-MDH1-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)


Characterization SOP Files

  NCI 60 Protein Array

CPTC-MDH1-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate
Click to enlarge image Western Blot using CPTC-MDH1-1 as primary Ab against cell lysate from transiently overexpressed HEK293T cells form Origene (lane 2). Also included are molecular wt. standards (lane 1), lysate from non-transfected HEK293T cells as neg control (lane 3) and recombinant Ag MDH1 (NCI 10990) in (lane 4). Click image to enlarge

CPTC-MDH1-1 Cell Lysate Western blot

Result: Positive

Western Blot using CPTC-MDH1-1 as primary Ab against cell lysate from transiently overexpressed HEK293T cells form Origene (lane 2). Also included are molecular wt. standards (lane 1), lysate from non-transfected HEK293T cells as neg control (lane 3) and recombinant Ag MDH1 (NCI 10990) in (lane 4).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-MDH1-1 as primary Ab against Ag 10990 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-MDH1-1 Western blot

Result: Positive

Western Blot using CPTC-MDH1-1 as primary Ab against Ag 10990 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antibody: CPTC-MDH1-2
Background

Catalog Number:

CPTC-MDH1-2

RRID:

AB_10804294

Target Antigen:

Malate Dehydrogenase 1 NAD (soluble)

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

11/14/2019

Antigen Recognition(s):

Recombinant Full-length, Endogenous

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (89.7 KB)

CPTC-MDH1-2 Evaluation by Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC NCI60

CPTC-MDH1-2 IHC NCI60

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IHC Tissue

CPTC-MDH1-2 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-MDH1-2 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)


Characterization SOP Files

  NCI 60 Protein Array

CPTC-MDH1-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate
Click to enlarge image Western Blot using CPTC-MDH1-2 as primary Ab against cell lysate from transiently overexpressed HEK293T cells form Origene (lane 2). Also included are molecular wt. standards (lane 1), lysate from non-transfected HEK293T cells as neg control (lane 3) and recombinant Ag MDH1 (NCI 10990) in (lane 4). Click image to enlarge

CPTC-MDH1-2 Cell Lysate Western blot

Result: Positive

Western Blot using CPTC-MDH1-2 as primary Ab against cell lysate from transiently overexpressed HEK293T cells form Origene (lane 2). Also included are molecular wt. standards (lane 1), lysate from non-transfected HEK293T cells as neg control (lane 3) and recombinant Ag MDH1 (NCI 10990) in (lane 4).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-MDH1-2 as primary Ab against Ag 10990 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-MDH1-2 Western blot

Result: Positive

Western Blot using CPTC-MDH1-2 as primary Ab against Ag 10990 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antibody: CPTC-MDH1-3
Background

Catalog Number:

CPTC-MDH1-3

RRID:

AB_10805425

Target Antigen:

Malate Dehydrogenase 1 NAD (soluble)

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

10/15/2010

Antigen Recognition(s):

Purchase
Characterization Data [Compare Characterization Data]
  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-MDH1-3 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-MDH1-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-MDH1-3 Protein Array

Result: Positive

Protein Array in which CPTC-MDH1-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-MDH1-3 as primary Ab against Ag 10990 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-MDH1-3 Western blot

Result: Positive

Western Blot using CPTC-MDH1-3 as primary Ab against Ag 10990 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-MDH1-3 as primary Ab against cell lysate from transiently overexpressed HEK293T cells form Origene (lane 2). Also included are molecular wt. standards (lane 1), lysate from non-transfected HEK293T cells as neg control (lane 3) and recombinant Ag MDH1 (NCI 10990) in (lane 4). Click image to enlarge

CPTC-MDH1-3 Cell Lysate Western blot

Result: Positive

Western Blot using CPTC-MDH1-3 as primary Ab against cell lysate from transiently overexpressed HEK293T cells form Origene (lane 2). Also included are molecular wt. standards (lane 1), lysate from non-transfected HEK293T cells as neg control (lane 3) and recombinant Ag MDH1 (NCI 10990) in (lane 4).


Characterization SOP Files

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Antigen: Malate Dehydrogenase 1 NAD (soluble)
Background

NCI Identification Number:

10990

Antigen Name:

Malate Dehydrogenase 1 NAD (soluble)

CPTC Name:

CPTC-MDH1

Aliases:

EC 1.1.1.37; MDH-s; MDHA; MGC:1375; MOR2

Function:

Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria.

Chromosomal Localization:

2p13.3

Expression System:

E. Coli

Accession Number:

BC001484.2

UniProt Accession Number:

P40925

DNA Source:

OB : MHS1011-169973

Immunogen:

Recombinant Full Length Protein

Vector Name:

pMCSG7

Extinction Coefficient:

32555

Buffers:

PBS

Expressed Sequence:

SNAMSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMM
GVLDGVLMELQDCALPLLKDVIATDKEDVAFKDLDVAILVGSMPRREGME
RKDLLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSI
PKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNH
AKVKLQGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAA
KAICDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTW
KFVEGLPINDFSREKMDLTAKELTEEKESAFEFLSSA

Native Sequence:

MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVL
DGVLMELQDCALPLLKDVIATDKEDVAFKDLDVAILVGSMPRREGMERKD
LLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKE
NFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNHAKV
KLQGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAAKAI
CDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFV
EGLPINDFSREKMDLTAKELTEEKESAFEFLSSA

Calculated Isoelectric Point:

6.91

Molecular Weight:

36.43

Last Updated:

08/22/2020

Links

Characterization Data

Gel

Click to enlarge image PAGE of MDH1 (Ag 10990) in Lane 2 with molecular weight standards in lane 1
Click image to enlarge

CPTC-MDH1 PAGE

PAGE of MDH1 (Ag 10990) in Lane 2 with molecular weight standards in lane 1

Characterization SOP Files

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SOPs

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