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Mediator Of DNA-Damage Checkpoint 1 Peptide 1

Antibody: CPTC-MDC1-1
Background

Catalog Number:

CPTC-MDC1-1

RRID:

AB_2722082

Target Antigen:

Mediator Of DNA-Damage Checkpoint 1 Peptide 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

09/01/2022

Antigen Recognition(s):

Peptide, Endogenous, Phosphorylation

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (442.4 KB)

CPTC-MDC1-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IP
Click to enlarge image Immuno-Precipitation Mass Spectrometry using CPTC-MDC1-1 antibody with CPTC-MDC1 peptide 1 (phosphorylated) as the target antigen. Click image to enlarge

CPTC-MDC1-1 IPMS (phosphorylated)

Result: Positive

Immuno-Precipitation Mass Spectrometry using CPTC-MDC1-1 antibody with CPTC-MDC1 peptide 1 (phosphorylated) as the target antigen.


Characterization SOP Files

  IP
Click to enlarge image Immuno-Precipitation Mass Spectrometry using CPTC-MDC1-1 antibody with CPTC-MDC1 peptide 1 (non-phosphorylated) as the target antigen. Click image to enlarge

CPTC-MDC1-1 IPMS (non-phosphorylated)

Result: Positive

Immuno-Precipitation Mass Spectrometry using CPTC-MDC1-1 antibody with CPTC-MDC1 peptide 1 (non-phosphorylated) as the target antigen.


Characterization SOP Files

  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (442.4 KB)

CPTC-MDC1-1 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-MDC1-1 Indirect ELISA (Phosphorylated)

Result: Positive

Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-MDC1-1 Indirect ELISA (non-phosphorylated)

Result: Positive

Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

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Antibody: CPTC-MDC1-2
Background

Catalog Number:

CPTC-MDC1-2

RRID:

AB_2722083

Target Antigen:

Mediator Of DNA-Damage Checkpoint 1 Peptide 1

Isotype:

IgG3

Species:

Mouse Monoclonal Antibody

Last Updated:

09/01/2022

Antigen Recognition(s):

Peptide, Phosphorylation

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (685.7 KB)

CPTC-MDC1-2 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IP
Click to enlarge image Immuno-Precipitation Mass Spectrometry using CPTC-MDC1-1 antibody with CPTC-MDC1 peptide 1 (phosphorylated) as the target antigen. Click image to enlarge

CPTC-MDC1-2 IPMS (phosphorylated)

Result: Positive

Immuno-Precipitation Mass Spectrometry using CPTC-MDC1-1 antibody with CPTC-MDC1 peptide 1 (phosphorylated) as the target antigen.


Characterization SOP Files

  IP
Click to enlarge image Immuno-Precipitation Mass Spectrometry using CPTC-MDC1-1 antibody with CPTC-MDC1 peptide 1 (non-phosphorylated) as the target antigen. Click image to enlarge

CPTC-MDC1-2 IPMS (non-phosphorylated)

Result: Positive

Immuno-Precipitation Mass Spectrometry using CPTC-MDC1-1 antibody with CPTC-MDC1 peptide 1 (non-phosphorylated) as the target antigen.


Characterization SOP Files

  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (685.7 KB)

CPTC-MDC1-2 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-MDC1-2 Indirect ELISA (Phosphorylated)

Result: Positive

Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-MDC1-2 Indirect ELISA (non-phosphorylated)

Result: Positive

Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

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Antibody: CPTC-MDC1-3
Background

Catalog Number:

CPTC-MDC1-3

RRID:

AB_2722084

Target Antigen:

Mediator Of DNA-Damage Checkpoint 1 Peptide 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

09/01/2022

Antigen Recognition(s):

Peptide, Phosphorylation

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (626.1 KB)

CPTC-MDC1-3 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IP
Click to enlarge image Immuno-Precipitation Mass Spectrometry using CPTC-MDC1-3 antibody with CPTC-MDC1 peptide 1 (phosphorylated) as the target antigen. Click image to enlarge

CPTC-MDC1-3 IPMS (phosphorylated)

Result: Positive

Immuno-Precipitation Mass Spectrometry using CPTC-MDC1-3 antibody with CPTC-MDC1 peptide 1 (phosphorylated) as the target antigen.


Characterization SOP Files

  IP
Click to enlarge image Immuno-Precipitation Mass Spectrometry using CPTC-MDC1-3 antibody with CPTC-MDC1 peptide 1 (non-phosphorylated) as the target antigen. Click image to enlarge

CPTC-MDC1-3 IPMS (non-phosphorylated)

Result: Positive

Immuno-Precipitation Mass Spectrometry using CPTC-MDC1-3 antibody with CPTC-MDC1 peptide 1 (non-phosphorylated) as the target antigen.


Characterization SOP Files

  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (626.1 KB)

CPTC-MDC1-3 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-MDC1-3 Indirect ELISA (Phosphorylated)

Result: Positive

Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-MDC1-3 Indirect ELISA (non-phosphorylated)

Result: Positive

Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

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Antibody: CPTC-MDC1-4
Background

Catalog Number:

CPTC-MDC1-4

RRID:

AB_2820266

Target Antigen:

Mediator Of DNA-Damage Checkpoint 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Endogenous, Phosphorylation

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

External Links
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (331.4 KB)

CPTC-MDC1-4 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immuno-MRM
Click to enlarge image mmuno-MRM chromatogram of CPTC-MDC1-4 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3235) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html) Click image to enlarge

CPTC-MDC1-4 iMRM

Result: Positive

mmuno-MRM chromatogram of CPTC-MDC1-4 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3235)

Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-MDC1-4 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3235) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysate. Click image to enlarge

CPTC-MDC1-4 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-MDC1-4 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3235)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysate.


Characterization SOP Files

  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (331.4 KB)

CPTC-MDC1-4 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

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Antibody: CPTC-MDC1-5
Background

Catalog Number:

CPTC-MDC1-5

Target Antigen:

Mediator Of DNA-Damage Checkpoint 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

09/01/2022

Antigen Recognition(s):

Peptide, Phosphorylation

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

External Links
Purchase
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (760.5 KB)

CPTC-MDC1-5 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immuno-MRM
Click to enlarge image mmuno-MRM chromatogram of CPTC-MDC1-5 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3234) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html) Click image to enlarge

CPTC-MDC1-5 iMRM

Result: Positive

mmuno-MRM chromatogram of CPTC-MDC1-5 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3234)

Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)


Characterization SOP Files

  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (760.5 KB)

CPTC-MDC1-5 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

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Antigen: Mediator Of DNA-Damage Checkpoint 1 Peptide 1
Background

NCI Identification Number:

00100

Antigen Name:

Mediator Of DNA-Damage Checkpoint 1 Peptide 1

CPTC Name:

CPTC-MDC1 Peptide 1

Aliases:

NFBD1; Nuclear Factor With BRCT Domains 1; KIAA0170; Homologue To Drosophila Photoreceptor Protein Calphotin; Mediator Of DNA Damage Checkpoint 1; Mediator Of DNA Damage Checkpoint Protein 1

Function:

The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a
central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.

xMDC1 (mediator of DNA-damage checkpoint 1) is a protein-coding gene. GO annotations related to this gene include protein C-terminus binding and FHA domain binding. An important paralog of this gene is PAXIP1.

Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.

Chromosomal Localization:

6p21.3

Accession Number:

NP_055456.2

UniProt Accession Number:

Q14676

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

AQPFGFID(pS)DTDAEEER (pS329)

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1870

Last Updated:

03/11/2015

Links

Characterization Data

SOPs

No SOPs available.

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