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Minichromosome Maintenance Complex Component 6 Peptide 1

Antibody: CPTC-MCM6-1
Background

Catalog Number:

CPTC-MCM6-1

Target Antigen:

Minichromosome Maintenance Complex Component 6 Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Endogenous, Phosphorylation

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (340.6 KB)

CPTC-MCM6-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC Tissue

CTPC-MCM6-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-MCM6-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5918 for non-phosphorylated peptide and https://assays.cancer.gov/CPTAC-5919 for phosphorylated peptide) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysate. Click image to enlarge

CPTC-MCM6-1 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-MCM6-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5918 for non-phosphorylated peptide and https://assays.cancer.gov/CPTAC-5919 for phosphorylated peptide)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysate.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-MCM6-1 as primary antibody against cell lysates HeLa, MCF10A, and LCL57. Samples from each cell line were irradiated with 10 Gy as shown in ‘+’ indicated lanes. Samples from each non-irradiated cell line were treated with alkaline phosphatase enzyme as shown in ‘-‘ indicated lanes. Molecular weight standards are included for each cell line. Click image to enlarge

CPTC-MCM6-1 automated western blot (cell lysate)

Result: Negative

Automated western blot using CPTC-MCM6-1 as primary antibody against cell lysates HeLa, MCF10A, and LCL57. Samples from each cell line were irradiated with 10 Gy as shown in ‘+’ indicated lanes. Samples from each non-irradiated cell line were treated with alkaline phosphatase enzyme as shown in ‘-‘ indicated lanes. Molecular weight standards are included for each cell line.


Characterization SOP Files

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Antigen: Minichromosome Maintenance Complex Component 6 Peptide 1
Background

NCI Identification Number:

00290

Antigen Name:

Minichromosome Maintenance Complex Component 6 Peptide 1

CPTC Name:

CPTC-MCM6 Peptide 1

Aliases:

Minichromosome Maintenance Complex Component 6; Minichromosome Maintenance Deficient (Mis5, S. Pombe) 6; DNA Replication Licensing Factor MCM6; P105MCM; MCM6 Minichromosome Maintenance Deficient 6 (MIS5 Homolog, S. Pombe) (S. Cerevisiae); MCM6 Minichromosome Maintenance Deficient 6 (MIS5 Homolog, S. Pombe ); Minichromosome Maintenance Deficient 6 Homolog (S. Cerevisiae); MIS5 Homolog (S.Pombe); EC 3.6.4.12; MCG40308; Mis5

Function:

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication. Single nucleotide polymorphisms in the intron regions of this gene are associated with differential transcriptional activation of the promoter of the neighboring lactase gene and, thereby, influence lactose intolerance in early adulthood.MCM6 (Minichromosome Maintenance Complex Component 6) is a Protein Coding gene. Diseases associated with MCM6 include Lactose Intolerance, Adult Type and Lactose Intolerance. Among its related pathways are Mitotic G1-G1/S phases and CDK-mediated phosphorylation and removal of Cdc6. Gene Ontology (GO) annotations related to this gene include identical protein binding and ATP-dependent DNA helicase activity. An important paralog of this gene is MCM7.Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

Chromosomal Localization:

2q21.3

Accession Number:

NP_005906.2

UniProt Accession Number:

Q14566

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

CEIESEIDS(ph)EEELINK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1760

Last Updated:

08/21/2020

Links

Characterization Data

SOPs

No SOPs available.

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