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Linker For Activation of T Cells Peptide 1

Antibody: CPTC-LAT-1
Background

Catalog Number:

CPTC-LAT-1

Target Antigen:

Linker For Activation of T Cells Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous, Phosphorylation

External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (269.5 KB)

CPTC-LAT-1 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC NCI60
Download Immuno-histochemistry of CPTC-LAT-1 for NCI60 Cell Line Array with titer 1:6250 0=NEGATIVE 1=WEAK(red) 2=MODERATE(blue) 3=STRONG(green) (62.0 KB)

CPTC-LAT-1 IHC NCI60

Result: Positive

Immuno-histochemistry of CPTC-LAT-1 for NCI60 Cell Line Array with titer 1:6250
0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green)


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array(TMA) core of pancreatic cancer showing cytoplasmic staining using Antibody CPTC-LAT-1. Titer: 1:6250 Click image to enlarge

CPTC-LAT-1 IHC Tissue

Result: Positive

Tissue Micro-Array(TMA) core of pancreatic cancer showing cytoplasmic staining using Antibody CPTC-LAT-1. Titer: 1:6250


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-LAT-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5889) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysate. Click image to enlarge

CPTC-LAT-1 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-LAT-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5889)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysate.


Characterization SOP Files

  Immunofluorescence

CPTC-LAT-1 Immunofluorescence

Result: Negative

Immunofluorescence staining of HeLa cells using CPTC-LAT-1 antibody. LAT protein expression was not detected.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-LAT-1 as primary antibody against human Lat recombinant protein (lane 2). Also included are molecular weight standards (lane 1). Click image to enlarge

CPTC-LAT-1 Western Blot with Recombinant Protein

Result: Positive

Western Blot using CPTC-LAT-1 as primary antibody against human Lat recombinant protein (lane 2). Also included are molecular weight standards (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-LAT-1 as primary antibody against cell lysates LCL57 (lane 2), HeLa (lane 3) and MCF10A (lane 4). Also included are molecular weight standards (lane 1). Click image to enlarge

CPTC-LAT-1 western blot (cell lysates)

Result: Positive

Western Blot using CPTC-LAT-1 as primary antibody against cell lysates LCL57 (lane 2), HeLa (lane 3) and MCF10A (lane 4). Also included are molecular weight standards (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-LAT-1 as primary antibody against cell lysates LCL57 (lane 2), HeLa (lane 3) and MCF10A (lane 4). Also included are molecular weight standards (lane 1). Click image to enlarge

CPTC-LAT-1 automated western blot (cell lysate)

Result: Negative

Western Blot using CPTC-LAT-1 as primary antibody against cell lysates LCL57 (lane 2), HeLa (lane 3) and MCF10A (lane 4). Also included are molecular weight standards (lane 1).


Characterization SOP Files

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Antigen: Linker For Activation of T Cells Peptide 1
Background

NCI Identification Number:

00300

Antigen Name:

Linker For Activation of T Cells Peptide 1

CPTC Name:

CPTC-LAT Peptide 1

Aliases:

Linker For Activation Of T Cells; Linker For Activation Of T Cells, Transmembrane Adaptor; Linker For Activation Of T-Cells Family Member 1; 36 KDa Phospho-Tyrosine Adapter Protein; P36-38; Pp36; 36 KDa Phospho-Tyrosine Adaptor Protein; IMD52; LAT1

Function:

he protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms. LAT (Linker For Activation Of T Cells) is a Protein Coding gene. Diseases associated with LAT include Immunodeficiency 52 and Cranial Nerve Palsy. Among its related pathways are RET signaling and G beta-gamma signalling through PI3Kgamma. Gene Ontology (GO) annotations related to this gene include protein kinase binding and SH3/SH2 adaptor activity.Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.

Chromosomal Localization:

16p11.2

Accession Number:

NP_055202.1

UniProt Accession Number:

O43561

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

CEYVNVS(ph)QELHPGAAK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1760

Last Updated:

08/21/2020

Links

Characterization Data

SOPs

No SOPs available.

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