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Integrin Subunit Alpha E Peptide 1

Antibody: CPTC-ITGAE-1
Background

Catalog Number:

CPTC-ITGAE-1

RRID:

AB_2889057

Target Antigen:

Integrin Subunit Alpha E Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

Thematic Panel(s):

Immuno-Oncology (IO)

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (281.7 KB)

CPTC-ITGAE-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-ITGAE-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5961) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool. Click image to enlarge

CPTC-ITGAE-1 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-ITGAE-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5961)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool.


Characterization SOP Files

  NCI 60 Protein Array

CTPC-ITGAE-1 NCI60 protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-ITGAE-1 as primary antibody against human integrin, alpha E (antigen CD103, mucosal lymphocyte antigen 1; alpha polypeptide) (ITGAE) recombinant protein (lane 2). Expected molecular weight - 128.1 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-ITGAE-1 Western Blot (recombinant protein)

Result: Positive

Western blot using CPTC-ITGAE-1 as primary antibody against human integrin, alpha E (antigen CD103, mucosal lymphocyte antigen 1; alpha polypeptide) (ITGAE) recombinant protein (lane 2). Expected molecular weight - 128.1 kDa.
Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-ITGAE-1 Automated Western Blot (Cell Lysate)

Result: Negative

Automated western blot using CPTC-ITGAE-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) whole cell lysates. Expected molecular weight - 130.1 kDa. Buffy coat and all cell lines are negative. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-ITGAE-1 Western Blot (Cell Lysate)

Result: Negative

Western blot using CPTC-ITGAE-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and H226 (lane 6) whole cell lysates. Expected molecular weight - 130.1 kDa. All cell lines are negative. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antigen: Integrin Subunit Alpha E Peptide 1
Background

NCI Identification Number:

00375

Antigen Name:

Integrin Subunit Alpha E Peptide 1

CPTC Name:

CPTC-ITGAE Peptide 1

Aliases:

Integrin Subunit Alpha E; Integrin, Alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1; Alpha Polypeptide); Human Mucosal Lymphocyte Antigen 1, Alpha Polypeptide; Mucosal Lymphocyte 1 Antigen; Integrin Alpha-IEL; Integrin Alpha-E; HML-1 Antigen; HUMINAE; CD103; Antigen CD103, Human Mucosal Lymphocyte Antigen 1; Alpha Polypeptide; Antigen CD103; CD103 Antigen; ITGAE

Function:

Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin, this protein forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen. This protein is preferentially expressed in human intestinal intraepithelial lymphocytes (IEL), and in addition to a role in adhesion, it may serve as an accessory molecule for IEL activation. ITGAE (Integrin Subunit Alpha E) is a Protein Coding gene. Diseases associated with ITGAE include Hairy Cell Leukemia and Splenic Marginal Zone Lymphoma. Among its related pathways are Integrin Pathway and Degradation of the extracellular matrix. An important paralog of this gene is ITGAM.Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers.

Chromosomal Localization:

17p13.2

Accession Number:

NP_002199.3

UniProt Accession Number:

P38570

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

VSYQLQTPEGQTDHPQPILDR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

2310

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

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