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Interleukin 18 Peptide 1

Antibody: CPTC-IL18-4
Background

Catalog Number:

CPTC-IL18-4

RRID:

AB_2617277

Target Antigen:

Interleukin 18 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Endogenous

Thematic Panel(s):

Immuno-Oncology (IO)

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Characterization Data [Compare Characterization Data]
  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-IL18-4 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-705) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from plasmal. Data collected from FFPE tumor tissue lysate pool are available on the CPTAC assay portal (https://assays.cancer.gov/CPTAC-5980) Click image to enlarge

CPTC-IL18-4 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-IL18-4 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-705)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from plasmal. Data collected from FFPE tumor tissue lysate pool are available on the CPTAC assay portal (https://assays.cancer.gov/CPTAC-5980)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-IL18 Peptide 1-4 peptide ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide

CPTC-IL18-4 Western Blot Recombinant Protein

Result: Negative

Western blot using CPTC-IL18-4 as primary antibody against human interleukin 18 (interferon-gamma-inducing factor) (IL18) recombinant protein (lane 2). Expected molecular weight - 22.1 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antigen: Interleukin 18 Peptide 1
Background

NCI Identification Number:

00032

Antigen Name:

Interleukin 18 Peptide 1

CPTC Name:

CPTC-IL18 Peptide 1

Aliases:

IL-18; IG1F; IL-1g; IL1F4; MGC12320; Interferon gamma inducing factor; Iboctadekin

Function:

Interleukin-18 (IL-18) is a cytokine produced by macrophages and other cells that belongs to the IL-1 superfamily. IL-18 works together with IL-12 to induce cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). After stimulation with IL-18, natural killer (NK) cells and certain T cells release another important cytokine called interferon gamma or type II interferon that plays an important role in activating the macrophages or other cells. Apart from its physiological role, IL-18 is also able to induce severe inflammatory reactions, which suggests its role in certain inflammatory disorders. The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity.

Chromosomal Localization:

11q22.2 - q22.3

Accession Number:

NM_001562

UniProt Accession Number:

Q14116

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

ISTLSCENK

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

990

Last Updated:

06/11/2013

Links

Characterization Data

SOPs

No SOPs available.

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