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Intercellular Adhesion Molecule 1 Peptide 1

Antibody: CPTC-ICAM1-1
Background

Catalog Number:

CPTC-ICAM1-1

Target Antigen:

Intercellular Adhesion Molecule 1 Peptide 1

Isotype:

IgG1

Species:

Mouse Recombinant Cloned Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

Thematic Panel(s):

Immuno-Oncology (IO)

External Links
Publications
Characterization Data [Compare Characterization Data]
  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-ICAM1-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5943) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool. Click image to enlarge

CPTC-ICAM1-1 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-ICAM1-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5943)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-ICAM1-1

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-ICAM1-1 as primary antibody against human intercellular adhesion molecule 1 (ICAM1) recombinant protein (lane 2). Expected molecular weight - 52.2 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-ICAM1-1 Western Blot Recombinant Protein

Result: Positive

Western blot using CPTC-ICAM1-1 as primary antibody against human intercellular adhesion molecule 1 (ICAM1) recombinant protein (lane 2). Expected molecular weight - 52.2 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-ICAM1-1 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), H226 (lane 6), and PBMC (lane 7) whole cell lysates. Expected molecular weight - 57.8 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-ICAM1-1 Western Blot Cell Lysate

Result: Negative

Western blot using CPTC-ICAM1-1 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), H226 (lane 6), and PBMC (lane 7) whole cell lysates. Expected molecular weight - 57.8 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-ICAM1-1 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), H226 (lane 6), and PBMC (lane 7) whole cell lysates. Expected molecular weight - 57.8 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-ICAM1-1 Automated Western Blot Cell Lysate

Result: Negative

Automated western blot using CPTC-ICAM1-1 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), H226 (lane 6), and PBMC (lane 7) whole cell lysates. Expected molecular weight - 57.8 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antigen: Intercellular Adhesion Molecule 1 Peptide 1
Background

NCI Identification Number:

00475

Antigen Name:

Intercellular Adhesion Molecule 1 Peptide 1

CPTC Name:

CPTC-ICAM1 Peptide 1

Aliases:

Intercellular Adhesion Molecule 1; Major Group Rhinovirus Receptor; ICAM-1; CD54; BB2; Intercellular Adhesion Molecule 1 (CD54), Human Rhinovirus Receptor; Epididymis Secretory Sperm Binding Protein; Cell Surface Glycoprotein P3.58; Human Rhinovirus Receptor; CD54 Antigen; P3.58; ICAM1

Function:

This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor.ICAM1 (Intercellular Adhesion Molecule 1) is a Protein Coding gene. Diseases associated with ICAM1 include Malaria and Hepatocellular Carcinoma. Among its related pathways are Interleukin-11 Signaling Pathway and Folate Metabolism. An important paralog of this gene is ICAM3.ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. ICAM1_HUMAN,P05362(Microbial infection) Acts as a receptor for major receptor group rhinovirus A-B capsid proteins. ICAM1_HUMAN,P05362(Microbial infection) Acts as a receptor for Coxsackievirus A21 capsid proteins. ICAM1_HUMAN,P05362(Microbial infection) Upon Kaposi's sarcoma-associated herpesvirus/HHV-8 infection, is degraded by viral E3 ubiquitin ligase MIR2, presumably to prevent lysis of infected cells by cytotoxic T-lymphocytes and NK cell. Cell adhesion molecules (CAMs) are a large family of transmembrane proteins that are involved in the binding of a cell to another cell or to the extracellular matrix. They have roles in cell proliferation, differentiation, motility, trafficking, apoptosis and tissue architecture.

Chromosomal Localization:

19p13.2

Accession Number:

NP_000192.2

UniProt Accession Number:

P05362

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

DGTFPLPIGESVTVTR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1760

Last Updated:

12/17/2020

Links

Characterization Data

SOPs

No SOPs available.

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