Catalog Number:
CPTC-GLO1-1
RRID:
AB_1553719
Target Antigen:
Lactoylglutathione Lyase
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
05/23/2024
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Result: Positive
Kinetic titration data for GLO1-1 Ab (615A3.1) using Biacore SPR method
Result: Positive
Imaging mass cytometry on prostate cancer tissue core using CPTC-GLO1-1 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (liver, bone marrow, spleen, placenta, prostate, colon, pancreas, breast, lung, testis, endometrium, appendix, and kidney) and cancer tissues (colon, breast, ovarian, lung, and prostate).
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Immuno-histochemistry of CPTC-GLO1-1 for NCI60 Cell Line Array at titer Titer: 1:50
0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green)
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Human assay: A-431 fixed with PFA, dilution: 1:50
Human assay: U-2 OS fixed with PFA, dilution: 1:50
Human assay: U-251 MG fixed with PFA, dilution: 1:50
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Result: Positive
Protein Array in which CPTC-GLO1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present. Analysis performed using a standard panel of samples. Antibody dilution: 1:250.
Result: Positive
Western Blot using CPTC-GLO1-1 as primary Ab against GLO1 (Ag 10246) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Result: Positive
Western Blot using CPTC-GLO1-1 as primary Ab against cell lysate from NCI-H522 cells (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Catalog Number:
CPTC-GLO1-2
RRID:
AB_1553721
Target Antigen:
Lactoylglutathione Lyase
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
09/09/2021
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Result: Positive
This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).
Result: Positive
Immunohistochemistry of CPTC-GLO1-2 for NCI60 Cell Line Array. Data scored as:
0=NEGATIVE
1=WEAK (red)
2=MODERATE (blue)
3=STRONG (green)
TITER: 1:2500
Result: Positive
Tissue Micro-Array(TMA) core of colon cancer showing cytoplasmic staining using Antibody CPTC-GLO1-2. Titer: 1:2500
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Result: Positive
Protein Array in which CPTC-GLO1-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Result: Positive
Western Blot using CPTC-GLO1-2 as primary Ab against GLO1 (Ag 10246) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Catalog Number:
CPTC-GLO1-3
RRID:
AB_2109905
Target Antigen:
Lactoylglutathione Lyase
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
05/23/2024
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Result: Positive
Kinetic titration data for GLO1-3 Ab (615A28.1) using Biacore SPR method
Result: Positive
Imaging mass cytometry on normal prostate tissue core using CPTC-GLO1-3 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (liver, bone marrow, spleen, placenta, prostate, colon, pancreas, breast, lung, testis, endometrium, appendix, and kidney) and cancer tissues (colon, breast, ovarian, lung, and prostate).
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Immuno-histochemistry of CPTC-GLO1-3 for NCI60 Cell Line Array at titer 1:100
0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green)
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol. Human assay: A-431 fixed with PFA, dilution: 1:200
Human assay: U-2 OS fixed with PFA, dilution: 1:200
Human assay: U-251 MG fixed with PFA, dilution: 1:200
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Result: Positive
Protein Array in which CPTC-AKR1B1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present. Analysis performed using a standard panel of samples. Antibody dilution: 1:250.
Result: Positive
Western Blot using CPTC-GLO1-3 as primary Ab against GLO1 (Ag 10246) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
NCI Identification Number:
10246
Antigen Name:
Lactoylglutathione Lyase
CPTC Name:
CPTC-GLO1
Aliases:
GLO1; glyoxalase 1; Aldoketomutase; EC 4.4.1.5; GLOD1; GLY1; Methylglyoxalase; GLX1; ketone-aldehyde mutase; S-D-lactoyl glutathione methyl glyoxal lyase; OTTHUMP00000016339
Function:
The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere.
Chromosomal Localization:
6p21.3 p21.1
Expression System:
E. Coli
Accession Number:
NM_006708
UniProt Accession Number:
Q04760
DNA Source:
HIP:HsCD00000982
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
25565
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMAEPQPPSGGLTDEAALSCCSDADPSTKDFLLQQTMLRVKDPKKSLD
FYTRVLGMTLIQKCDFPIMKFSLYFLAYEDKNDIPKEKDEKIAWALSRKA
TLELTHNWGTEDDATQSYHNGNSDPRGFGHIGIAVPDVYSACKRFEELGV
KFVKKPDDGKMKGLAFIQDPDGYWIEILNPNKMATLM
Native Sequence:
MAEPQPPSGGLTDEAALSCCSDADPSTKDFLLQQTMLRVKDPKKSLDFYT
RVLGMTLIQKCDFPIMKFSLYFLAYEDKNDIPKEKDEKIAWALSRKATLE
LTHNWGTEDDATQSYHNGNSDPRGFGHIGIAVPDVYSACKRFEELGVKFV
KKPDDGKMKGLAFIQDPDGYWIEILNPNKMATLM
Calculated Isoelectric Point:
5.24
Molecular Weight:
20992
Last Updated:
08/22/2020
SDS-PAGE of CPTC-GLO1 with molecular weight markers (in Lane 1)
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