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Ectonucleoside Triphosphate Diphosphohydrolase 1 Peptide 1

Antibody: CPTC-ENTPD1-1
Background

Catalog Number:

CPTC-ENTPD1-1

RRID:

AB_2889055

Target Antigen:

Ectonucleoside Triphosphate Diphosphohydrolase 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Endogenous

Thematic Panel(s):

Immuno-Oncology (IO)

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (354.2 KB)

CPTC-ENTPD1-1 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of colon cancer showing cytoplasmic and membranous staining using Antibody CPTC-ENTPD1-1. Titer: 1:1000 Click image to enlarge

CTPC-ENTPD1-1 IHC Tissue

Result: Positive

Tissue Micro-Array (TMA) core of colon cancer showing cytoplasmic and membranous staining using Antibody CPTC-ENTPD1-1. Titer: 1:1000


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-ENTPD1-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5966) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool. Click image to enlarge

CPTC-ENTPD1-1 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-ENTPD1-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5966)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool.


Characterization SOP Files

  Immunofluorescence

CPTC-ENTPD1-1 Immunofluorescence

Result: Negative

Immunofluorescence staining of human cell lines Jurkat, MCF7 and NCI H226 with CPTC-ENTPD1-1 Ab shows no localization of ENTPD1 protein.


Characterization SOP Files

  NCI 60 Protein Array

CTPC-ENTPD1-1 NCI60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-ENTPD1-1 as primary antibody against human ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1) recombinant protein (lane 2). Expected molecular weight - 57.8 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-ENTPD1-1 Western Blot (recombinant protein)

Result: Positive

Western blot using CPTC-ENTPD1-1 as primary antibody against human ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1) recombinant protein (lane 2). Expected molecular weight - 57.8 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-ENTPD1-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) cell lysates. Expected molecular weight - 57.8 kDa. Molecular weight standards are also included (lane 1). Inconclusive data. Click image to enlarge

CPTC-ENTPD1-1 Automated Western Blot (Cell Lysate)

Result: Negative

Automated western blot using CPTC-ENTPD1-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) cell lysates. Expected molecular weight - 57.8 kDa. Molecular weight standards are also included (lane 1). Inconclusive data.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-ENTPD1-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5) and NCI H226 (lane 6) cell lysates. Expected molecular weight 58 kDa. Molecular weight standards (MW Stds.) are also included (lane 1). Positive for cell line NCI H226. Negative/ inconclusive data for the other cell lines. Click image to enlarge

CPTC-ENTPD1-1 Western Blot (Cell Lysate)

Result: Positive

Western blot using CPTC-ENTPD1-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5) and NCI H226 (lane 6) cell lysates. Expected molecular weight 58 kDa. Molecular weight standards (MW Stds.) are also included (lane 1). Positive for cell line NCI H226. Negative/ inconclusive data for the other cell lines.


Characterization SOP Files

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Antigen: Ectonucleoside Triphosphate Diphosphohydrolase 1 Peptide 1
Background

NCI Identification Number:

00363

Antigen Name:

Ectonucleoside Triphosphate Diphosphohydrolase 1 Peptide 1

CPTC Name:

CPTC-ENTPD1 Peptide 1

Aliases:

Ectonucleoside Triphosphate Diphosphohydrolase 1; Lymphoid Cell Activation Antigen; Ecto-ATP Diphosphohydrolase 1; Ecto-ATPDase 1; Ecto-ATPase 1; CD39 Antigen; Ecto-Apyrase; EC 3.6.1.5; NTPDase-1; ATPDase; SPG64; CD39; NTPDase 1; EC 3.6.1; ENTPD1

Function:

The protein encoded by this gene is a plasma membrane protein that hydrolyzes extracellular ATP and ADP to AMP. Inhibition of this protein's activity may confer anticancer benefits. Several transcript variants encoding different isoforms have been found for this gene.ENTPD1 (Ectonucleoside Triphosphate Diphosphohydrolase 1) is a Protein Coding gene. Diseases associated with ENTPD1 include Spastic Paraplegia 64, Autosomal Recessive and Paraplegia. Among its related pathways are ATP/ITP metabolism and Metabolism of nucleotides. Gene Ontology (GO) annotations related to this gene include hydrolase activity and nucleoside-diphosphatase activity. An important paralog of this gene is ENTPD8.In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well.

Chromosomal Localization:

Accession Number:

NP_001767.3

UniProt Accession Number:

P49961

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

SLSNYPFDFQGAR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1430

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

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