Catalog Number:
CPTC-EBAG9-1
RRID:
AB_2617249
Target Antigen:
Estrogen Receptor Binding Site Associated, Antigen 9
Isotype:
IgG2c
Species:
Mouse Monoclonal Antibody
Last Updated:
05/28/2024
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Result: Positive
Imaging mass cytometry on normal endometrium tissue core using CPTC-EBAG9-1 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (liver, bone marrow, colon, pancreas, breast, lung, testis, endometrium, appendix, and kidneys) and cancer tissues (lung, breast, ovarian, and colon).
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Tissue Micro-Array(TMA) core of breast cancer showing cytoplasmic staining using Antibody CPTC-EBAG9-1. Titer: 1:4000
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus. Human assay: HeLa fixed with PFA, dilution: 1:2000
Human assay: MCF7 fixed with PFA, dilution: 1:2000
Human assay: U-2 OS fixed with PFA, dilution: 1:2000
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Result: Positive
Western Blot using CPTC-EBAG9-1 as primary Ab against EBAG9 (rAg 10772) in lane 3. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 2).
Catalog Number:
CPTC-EBAG9-2
Target Antigen:
Estrogen Receptor Binding Site Associated, Antigen 9
Isotype:
IgG2a
Species:
Mouse Monoclonal Antibody
Last Updated:
09/09/2021
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus. Human assay: HeLa fixed with PFA, dilution: 1:2000
Human assay: MCF7 fixed with PFA, dilution: 1:2000
Human assay: U-2 OS fixed with PFA, dilution: 1:2000
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present. Analysis performed using a standard panel of samples. Antibody dilution: 1:500
Result: Positive
Western Blot using CPTC-EBAG9-2 as primary Ab against EBAG9 (rAg 10772) in lane 3. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 2).
Catalog Number:
CPTC-EBAG9-3
RRID:
AB_2617251
Target Antigen:
Estrogen Receptor Binding Site Associated, Antigen 9
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
09/09/2021
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus. Human assay: HeLa fixed with PFA, dilution: 1:2000
Human assay: MCF7 fixed with PFA, dilution: 1:2000
Human assay: U-2 OS fixed with PFA, dilution: 1:2000
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Result: Positive
Western Blot using CPTC-EBAG9-3 as primary Ab against EBAG9 (rAg 10772) in lane 3. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 2).
NCI Identification Number:
10772
Antigen Name:
Estrogen Receptor Binding Site Associated, Antigen 9
CPTC Name:
CPTC-EBAG9
Aliases:
EB9; PDAF; RCAS1
Function:
This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor which binds to the estrogen-responsive element (ERE) found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene.
May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.
Chromosomal Localization:
8q23
Expression System:
E. Coli
Accession Number:
BC017729.1
UniProt Accession Number:
O00559
DNA Source:
HIP : HsCD00040328
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
30480
Buffers:
PBS
Expressed Sequence:
SNAGRGRKLSGDQITLPTTVDYSSVPKQTDVEEWTSWDEDAPTSVKIEGG
NGNVATQQNSLEQLEPDYFKDMTPTIRKTQKIVIKKRPLNFGIPDGSTGF
SSRLAATQDLPFIHQSSELGDLDTWQENTNAWEEEEDAAWQAEEVLRQQK
LADREKRAAEQQRKKMEKEAQRMKKEQNKIGVKLS
Native Sequence:
MAITQFRLFKFCTCLATVFSFLKRLICRSGRGRKLSGDQITLPTTVDYSS
VPKQTDVEEWTSWDEDAPTSVKIEGGNGNVATQQNSLEQLEPDYFKDMTP
TIRKTQKIVIKKREPLNFGIPDGSTGFSSRLAATQDLPFIHQSSELGDLD
TWQENTNAWEEEEDAAWQAEEVLRQQKLADREKRAAEQQRKKMEKEAQRL
MKKEQNKIGVKLS
Calculated Isoelectric Point:
5.06
Molecular Weight:
20950
Last Updated:
11/06/2019
PAGE of EBAG9 (rAg 10772) with molecular weight standards (lane 1)
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