PRINT

Estrogen Receptor Binding Site Associated, Antigen 9

Antibody: CPTC-EBAG9-1
Background

Catalog Number:

CPTC-EBAG9-1

RRID:

AB_2617249

Target Antigen:

Estrogen Receptor Binding Site Associated, Antigen 9

Isotype:

IgG2c

Species:

Mouse Monoclonal Antibody

Last Updated:

05/28/2024

Antigen Recognition(s):

Recombinant Full-length, Endogenous

SOP:

Purchase
External Links
Characterization Data [Compare Characterization Data]
  CyTOF
Click to enlarge image Imaging mass cytometry on normal endometrium tissue core using CPTC-EBAG9-1 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (liver, bone marrow, colon, pancreas, breast, lung, testis, endometrium, appendix, and kidneys) and cancer tissues (lung, breast, ovarian, and colon). Click image to enlarge

CPTC-EBAG9-1 Immuno-Mass Cytometry

Result: Positive

Imaging mass cytometry on normal endometrium tissue core using CPTC-EBAG9-1 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (liver, bone marrow, colon, pancreas, breast, lung, testis, endometrium, appendix, and kidneys) and cancer tissues (lung, breast, ovarian, and colon).


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (174.8 KB)

CPTC-EBAG9-1 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array(TMA) core of breast cancer showing cytoplasmic staining using Antibody CPTC-EBAG9-1. Titer: 1:4000 Click image to enlarge

CPTC-EBAG9-1 IHC Tissue

Result: Positive

Tissue Micro-Array(TMA) core of breast cancer showing cytoplasmic staining using Antibody CPTC-EBAG9-1. Titer: 1:4000


Characterization SOP Files

  Immunofluorescence - HPA
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus. Human assay: HeLa fixed with PFA, dilution: 1:2000 Human assay: MCF7 fixed with PFA, dilution: 1:2000 Human assay: U-2 OS fixed with PFA, dilution: 1:2000 Click image to enlarge

CPTC-EBAG9-1 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus. Human assay: HeLa fixed with PFA, dilution: 1:2000
Human assay: MCF7 fixed with PFA, dilution: 1:2000
Human assay: U-2 OS fixed with PFA, dilution: 1:2000


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-EBAG9-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-EBAG9-1NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-EBAG9-1 as primary Ab against EBAG9 (rAg 10772) in lane 3. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 2). Click image to enlarge

CPTC-EBAG9-1 Western blot

Result: Positive

Western Blot using CPTC-EBAG9-1 as primary Ab against EBAG9 (rAg 10772) in lane 3. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 2).


Characterization SOP Files

[top]

Antibody: CPTC-EBAG9-2
Background

Catalog Number:

CPTC-EBAG9-2

Target Antigen:

Estrogen Receptor Binding Site Associated, Antigen 9

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

09/09/2021

Antigen Recognition(s):

Recombinant Full-length, Endogenous

SOP:

Purchase
External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (175.0 KB)

CPTC-EBAG9-2 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immunofluorescence - HPA
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus. Human assay: HeLa fixed with PFA, dilution: 1:2000 Human assay: MCF7 fixed with PFA, dilution: 1:2000 Human assay: U-2 OS fixed with PFA, dilution: 1:2000 Click image to enlarge

CPTC-EBAG9-2 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus. Human assay: HeLa fixed with PFA, dilution: 1:2000
Human assay: MCF7 fixed with PFA, dilution: 1:2000
Human assay: U-2 OS fixed with PFA, dilution: 1:2000


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-EBAG9-2 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Western Blot - HPA - tissue or cell lysate
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present. Analysis performed using a standard panel of samples. Antibody dilution: 1:500 Click image to enlarge

CPTC-EBAG9-2 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present. Analysis performed using a standard panel of samples. Antibody dilution: 1:500


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-EBAG9-2 as primary Ab against EBAG9 (rAg 10772) in lane 3. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 2). Click image to enlarge

CPTC-EBAG9-2 Western blot

Result: Positive

Western Blot using CPTC-EBAG9-2 as primary Ab against EBAG9 (rAg 10772) in lane 3. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 2).


Characterization SOP Files

[top]

Antibody: CPTC-EBAG9-3
Background

Catalog Number:

CPTC-EBAG9-3

RRID:

AB_2617251

Target Antigen:

Estrogen Receptor Binding Site Associated, Antigen 9

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

09/09/2021

Antigen Recognition(s):

Recombinant Full-length, Endogenous

SOP:

Purchase
External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (175.0 KB)

CPTC-EBAG9-3 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC NCI60

CPTC-EBAG9-3 IHC NCI60

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IHC Tissue

CPTC-EBAG9-3 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Immunofluorescence - HPA
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus. Human assay: HeLa fixed with PFA, dilution: 1:2000 Human assay: MCF7 fixed with PFA, dilution: 1:2000 Human assay: U-2 OS fixed with PFA, dilution: 1:2000 Click image to enlarge

CPTC-EBAG9-3 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus. Human assay: HeLa fixed with PFA, dilution: 1:2000
Human assay: MCF7 fixed with PFA, dilution: 1:2000
Human assay: U-2 OS fixed with PFA, dilution: 1:2000


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-EBAG9-3 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-EBAG9-3 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-EBAG9-3 as primary Ab against EBAG9 (rAg 10772) in lane 3. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 2). Click image to enlarge

CPTC-EBAG9-3 Western blot

Result: Positive

Western Blot using CPTC-EBAG9-3 as primary Ab against EBAG9 (rAg 10772) in lane 3. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 2).


Characterization SOP Files

[top]

Antigen: Estrogen Receptor Binding Site Associated, Antigen 9
Background

NCI Identification Number:

10772

Antigen Name:

Estrogen Receptor Binding Site Associated, Antigen 9

CPTC Name:

CPTC-EBAG9

Aliases:

EB9; PDAF; RCAS1

Function:

This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor which binds to the estrogen-responsive element (ERE) found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene.

May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.

Chromosomal Localization:

8q23

Expression System:

E. Coli

Accession Number:

BC017729.1

UniProt Accession Number:

O00559

DNA Source:

HIP : HsCD00040328

Immunogen:

Recombinant Full Length Protein

Vector Name:

pMCSG7

Extinction Coefficient:

30480

Buffers:

PBS

Expressed Sequence:

SNAGRGRKLSGDQITLPTTVDYSSVPKQTDVEEWTSWDEDAPTSVKIEGG
NGNVATQQNSLEQLEPDYFKDMTPTIRKTQKIVIKKRPLNFGIPDGSTGF
SSRLAATQDLPFIHQSSELGDLDTWQENTNAWEEEEDAAWQAEEVLRQQK
LADREKRAAEQQRKKMEKEAQRMKKEQNKIGVKLS

Native Sequence:

MAITQFRLFKFCTCLATVFSFLKRLICRSGRGRKLSGDQITLPTTVDYSS
VPKQTDVEEWTSWDEDAPTSVKIEGGNGNVATQQNSLEQLEPDYFKDMTP
TIRKTQKIVIKKREPLNFGIPDGSTGFSSRLAATQDLPFIHQSSELGDLD
TWQENTNAWEEEEDAAWQAEEVLRQQKLADREKRAAEQQRKKMEKEAQRL
MKKEQNKIGVKLS

Calculated Isoelectric Point:

5.06

Molecular Weight:

20950

Last Updated:

11/06/2019

Links

Characterization Data

Gel

Click to enlarge image PAGE of EBAG9 (rAg 10772) with molecular weight standards (lane 1)
Click image to enlarge

CPTC-EBAG9 PAGE

PAGE of EBAG9 (rAg 10772) with molecular weight standards (lane 1)

[top]

SOPs

[top]

Don't have Adobe Reader™?

Get it for free at Adobe.com

  [top]