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C-X3-C Motif Chemokine Ligand 1 Peptide 1

Antibody: CPTC-CX3CL1-1
Background

Catalog Number:

CPTC-CX3CL1-1

Target Antigen:

C-X3-C Motif Chemokine Ligand 1 Peptide 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Endogenous

Thematic Panel(s):

Immuno-Oncology (IO)

External Links
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (411.4 KB)

CPTC-CX3CL1-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-CX3CL1-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5967) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool. Click image to enlarge

CPTC-CX3CL1-1 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-CX3CL1-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5967)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool.


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (411.4 KB)

CPTC-CX3CL1-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  NCI 60 Protein Array

CPTC-CX3CL1-1

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-CX3CL1-1 as primary antibody against human chemokine (C-X3-C motif) ligand 1 (CX3CL1) recombinant protein (lane 2). Expected molecular weight - 34.4 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-CX3CL1-1 Western Blot Recombinant Protein

Result: Positive

Western blot using CPTC-CX3CL1-1 as primary antibody against human chemokine (C-X3-C motif) ligand 1 (CX3CL1) recombinant protein (lane 2). Expected molecular weight - 34.4 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-CX3CL1-1 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), H226 (lane 6), and PBMC (lane 7) whole cell lysates. Expected molecular weight - 42.2 kDa. Molecular weight standards are also included (lane 1). A549 is presumed positive. All other cell lines are negative. Target protein is subject to glycosylation which can affect the migration in electrophoresis. This can make the target appear as a higher molecular weight protein. Click image to enlarge

CPTC-CX3CL1-1 Western Blot Cell Lysate

Result: Presumed Positive (with additional bands)

Western blot using CPTC-CX3CL1-1 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), H226 (lane 6), and PBMC (lane 7) whole cell lysates. Expected molecular weight - 42.2 kDa. Molecular weight standards are also included (lane 1). A549 is presumed positive. All other cell lines are negative. Target protein is subject to glycosylation which can affect the migration in electrophoresis. This can make the target appear as a higher molecular weight protein.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-CX3CL1-1 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), H226 (lane 6), and PBMC (lane 7) whole cell lysates. Expected molecular weight - 42.2 kDa. Molecular weight standards are also included (lane 1). A549 is presumed positive. All other cell lines are negative. Target protein is subject to glycosylation which can affect the migration in electrophoresis. This can make the target appear as a higher molecular weight protein. Click image to enlarge

CPTC-CX3CL1-1 Automated Western Blot Cell Lysate

Result: Presumed Positive (with additional bands)

Automated western blot using CPTC-CX3CL1-1 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), H226 (lane 6), and PBMC (lane 7) whole cell lysates. Expected molecular weight - 42.2 kDa. Molecular weight standards are also included (lane 1). A549 is presumed positive. All other cell lines are negative. Target protein is subject to glycosylation which can affect the migration in electrophoresis. This can make the target appear as a higher molecular weight protein.


Characterization SOP Files

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Antibody: CPTC-CX3CL1-2
Background

Catalog Number:

CPTC-CX3CL1-2

Target Antigen:

C-X3-C Motif Chemokine Ligand 1 Peptide 1

Isotype:

IgG

Species:

Mouse Monoclonal Antibody

Last Updated:

10/31/2023

Antigen Recognition(s):

Peptide

Characterization Data [Compare Characterization Data]
  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (451.5 KB)

CPTC-CX3CL1-2 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (451.5 KB)

CPTC-CX3CL1-2 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

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Antibody: CPTC-CX3CL1-3
Background

Catalog Number:

CPTC-CX3CL1-3

Target Antigen:

C-X3-C Motif Chemokine Ligand 1 Peptide 1

Isotype:

IgG

Species:

Mouse Monoclonal Antibody

Last Updated:

02/02/2024

Antigen Recognition(s):

Peptide, Endogenous

External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (474.3 KB)

CPTC-CX3CL1-3 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Immunofluorescence - HPA
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line THP-1 shows localization to plasma membrane. Human assay: THP-1 fixed with PFA, dilution: 1:200 Human assay: U2OS fixed with PFA, dilution: 1:200 Click image to enlarge

CPTC-CX3CL1-3 evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line THP-1 shows localization to plasma membrane.


Human assay: THP-1 fixed with PFA, dilution: 1:200
Human assay: U2OS fixed with PFA, dilution: 1:200


Characterization SOP Files

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Antigen: C-X3-C Motif Chemokine Ligand 1 Peptide 1
Background

NCI Identification Number:

00472

Antigen Name:

C-X3-C Motif Chemokine Ligand 1 Peptide 1

CPTC Name:

CPTC-CX3CL1 Peptide 1

Aliases:

C-X3-C Motif Chemokine Ligand 1; Fractalkine; Neurotactin; Small Inducible Cytokine Subfamily D (Cys-X3-Cys), Member 1 (Fractalkine, Neurotactin); Chemokine (C-X3-C Motif) Ligand 1; CX3C Membrane-Anchored Chemokine; Small-Inducible Cytokine D1; C-X3-C Motif Chemokine 1; C3Xkine; ABCD-3; CXC3C; SCYD1; CXC3; NTN; NTT; Small Inducible Cytokine Subfamily D (Cys-X3-Cys), Member-1; CX3CL1; FKN

Function:

This gene belongs to the CX3C subgroup of chemokines, characterized by the number of amino acids located between the conserved cysteine residues. This is the only member of the CX3C subgroup, which contains three amino acids between cysteine residues, resulting in a Cys-X-X-X-Cys configuration. The encoded protein contains an extended mucin-like stalk with a chemokine domain on top, and exists in both a membrane-anchored form where it acts as a binding molecule, or, in soluble form, as a chemotactic cytokine. The mature form of this protein can be cleaved at the cell surface, yielding different soluble forms that can interact with the G-protein coupled receptor, C-X3-C motif chemokine receptor 1 gene product. This gene plays a role in a wide range of diseases, including cancer, vasculitis, neuropathies, atherosclerosis, inflammatory diseases, and in human immunodeficiency virus infections.CX3CL1 (C-X3-C Motif Chemokine Ligand 1) is a Protein Coding gene. Diseases associated with CX3CL1 include Pityriasis Rosea and Vasculitis. Among its related pathways are Signaling by GPCR and PEDF Induced Signaling. Gene Ontology (GO) annotations related to this gene include signaling receptor binding and chemokine activity. An important paralog of this gene is CCL21.Acts as a ligand for both CX3CR1 and integrins. Binds to CX3CR1 (PubMed:23125415, PubMed:9931005, PubMed:21829356). Binds to integrins ITGAV:ITGB3 and ITGA4:ITGB1. Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins. In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1 (PubMed:23125415, PubMed:24789099). The soluble form is chemotactic for T-cells and monocytes and not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium.

Chromosomal Localization:

16q21

Accession Number:

NP_002987.1

UniProt Accession Number:

P78423

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

ALGTSPELPTGVTGSSGTR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

2090

Last Updated:

12/16/2020

Links

Characterization Data

SOPs

No SOPs available.

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