Background
Catalog Number:
CPTC-CTLA4-1
RRID:
AB_2889959
Target Antigen:
Cytotoxic T-Lymphocyte Associated Protein 4 Peptide 1
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
07/03/2024
Antigen Recognition(s):
Peptide, Recombinant Full-length, Endogenous
External Links
- CPTAC Assay Portal CPTC-CTLA4-1
Purchase
- DSHB CPTC-CTLA4-1
Characterization Data [Compare Characterization Data]
IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (473.0 KB)
CPTC-CTLA4-1 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
Immuno-MRM
CPTC-CTLA4-1 iMRM (Frozen Tissue)
Result: Positive
Immuno-MRM chromatogram of CPTC-CTLA4-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6215)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue
Characterization SOP Files
Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (473.0 KB)
CPTC-CTLA4-1 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
NCI 60 Protein Array
CTPC-CTLA4-1 NCI60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-CTLA4-1 Western Blot (Recombinant Protein)
Result: Positive
Western blot using CPTC-CTLA4-1 as primary antibody against human cytotoxic T-lymphocyte-associated protein 4 (CTLA4), transcript variant 1, with C-terminal DDK/His tag, purified recombinant protein expressed in human cells (lane 2). Expected molecular weight - 16.0 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-CTLA4-1 Western Blot (Cell Lysate)
Result: Negative
Western blot using CPTC-CTLA4-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and H226 (lane 6) whole cell lysates. Expected molecular weight - 24.7 kDa, 6.6 kDa, 6.7 kDa, 8.9 kDa, and 19.1 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-CTLA4-1 Automated Western Blot (Cell Lysate)
Result: Negative
Automated western blot using CPTC-CTLA4-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) whole cell lysates. Expected molecular weight - 24.7 kDa, 6.6 kDa, 6.7 kDa, 8.9 kDa, and 19.1 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Background
NCI Identification Number:
00405
Antigen Name:
Cytotoxic T-Lymphocyte Associated Protein 4 Peptide 1
CPTC Name:
CPTC-CTLA4 Peptide 1
Aliases:
Cytotoxic T-Lymphocyte Associated Protein 4; CTLA-4; CD152; Insulin-Dependent Diabetes Mellitus 12; Cytotoxic T-Lymphocyte Protein 4; Celiac Disease 3; GSE; CD; Ligand And Transmembrane Spliced Cytotoxic T Lymphocyte Associated Antigen 4; Cytotoxic T Lymphocyte Associated Antigen 4 Short Spliced Form; Cytotoxic T-Lymphocyte-Associated Serine Esterase-4; Cytotoxic T-Lymphocyte-Associated Antigen 4; CD152 Isoform; CD152 Antigen; CELIAC3; IDDM12; ALPS5; CTLA4; GRD4
Function:
This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.CTLA4 (Cytotoxic T-Lymphocyte Associated Protein 4) is a Protein Coding gene. Diseases associated with CTLA4 include Autoimmune Lymphoproliferative Syndrome, Type V and Celiac Disease 3. Among its related pathways are TCR Signaling (Qiagen) and T cell receptor signaling pathway. An important paralog of this gene is CD28.Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
Chromosomal Localization:
2q33.2
Accession Number:
NP_005205.2
UniProt Accession Number:
P16410
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
GIASFVCEYASPGK
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
1540
Last Updated:
09/03/2020
Links
- EnsEMBL - EnsEMBL CTLA4
- Entrez - Entrez CTLA4
- Uniprot - Uniprot P16410
Characterization Data
SOPs
No SOPs available.
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