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Clusterin Peptide 1

Antibody: CPTC-CLU-1
Background

Catalog Number:

CPTC-CLU-1

RRID:

AB_2888948

Target Antigen:

Clusterin Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (159.8 KB)

CPTC-CLU-1 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-CLU-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5894) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from plasma. Click image to enlarge

CPTC-CLU-1 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-CLU-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5894)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from plasma.


Characterization SOP Files

  Immunofluorescence

CPTC-CLU-1 Immunofluorescence

Result: Negative

Immunofluorescence staining of MCF7 cells using CPTC-CLU-1 antibody. CLU protein expression was not detected.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Peptide coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-CLU-1 Indirect ELISA (peptide)

Result: Positive

Indirect ELISA (ie, binding of Antibody to Peptide coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-CLU-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-CLU-1 as primary Ab against HEK293T cell lysate containing CLU (from Origene) in lane 2. Also included are molecular wt. standards (lane 1) and the CLU-1 Ab as the IgG control (lane 3). Click image to enlarge

CPTC-CLU-1 Western blot (cell lysate)

Result: Positive

Western Blot using CPTC-CLU-1 as primary Ab against HEK293T cell lysate containing CLU (from Origene) in lane 2. Also included are molecular wt. standards (lane 1) and the CLU-1 Ab as the IgG control (lane 3).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-CLU-1 as primary Ab against lysates from six breast cancer cell lines from the NCI60 cell line collection (lanes 2-7). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-CLU-1 NCI60 Western blot

Result: Positive

Western Blot using CPTC-CLU-1 as primary Ab against lysates from six breast cancer cell lines from the NCI60 cell line collection (lanes 2-7). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

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Antigen: Clusterin Peptide 1
Background

NCI Identification Number:

00053

Antigen Name:

Clusterin Peptide 1

CPTC Name:

CPTC-CLU Peptide 1

Aliases:

CLI; APOJ1; KUB1; TRPM-2; CLU1; CLU2; SGP-2; SP-40; Apolipoprotein J; Complement Cytolysis Inhibitor; Complement-Associated Protein SP-40,40; Ku70-Binding Protein 1; Testosterone-Repressed Prostate Message 2; NA1/NA2; SGP2; TRPM2; Clusterin (Complement Lysis Inhibitor, SP-40,40; Clusterin (Complement Lysis Inhibitor, SP-40,40, Sulfated Glycoprotein 2,Testosterone-Repressed Prostate Message 2, Apolipoprotein J); APO-J; Aging-Associated Protein 4; Complement Lysis Inhibitor; Sulfated Glycoprotein 2; Aging-Associated Gene 4 Protein

Function:

The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the
cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor
progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variant.

Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA,
CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by
complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein
ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote
apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.

Chromosomal Localization:

8p21-p12

Accession Number:

NP_001822.3

UniProt Accession Number:

P10909

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

ASSIIDELFQDR

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1320

Last Updated:

06/21/2013

Links

Characterization Data

SOPs

No SOPs available.

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