Background
Catalog Number:
CPTC-CEACAM8-1
Target Antigen:
CEA Cell Adhesion Molecule 8 Peptide 1
Isotype:
IgG
Species:
Mouse Monoclonal Antibody
Last Updated:
10/20/2023
Antigen Recognition(s):
Peptide
Characterization Data [Compare Characterization Data]
IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (376.7 KB)
CPTC-CEACAM8-1 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (376.7 KB)
CPTC-CEACAM8-1 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
Background
Catalog Number:
CPTC-CEACAM8-2
Target Antigen:
CEA Cell Adhesion Molecule 8 Peptide 1
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
03/20/2024
Antigen Recognition(s):
Peptide, Endogenous
Thematic Panel(s):
Immuno-Oncology (IO)
External Links
- CPTAC Assay Portal CPTAC-5958
- Human Protein Atlas CPTC-CEACAM8-2
Publications
Characterization Data [Compare Characterization Data]
IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (531.8 KB)
CPTC-CEACAM8-2 evaluation by the Human Protein Atlas
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
Immuno-MRM
CPTC-CEACAM8-2 iMRM
Result: Positive
Immuno-MRM chromatogram of CPTC-CEACAM8-2 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5958)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool.
Characterization SOP Files
Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (531.8 KB)
CPTC-CEACAM8-2 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
NCI 60 Protein Array
CPTC-CEACAM8-2 NCI 60 Protein Array
Result: Positive
Protein Array in which CPTC-CEACAM8-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-CEACAM8-2 Western Blot Recombinant Protein
Result: Negative
Western blot using CPTC-CEACAM8-2 as primary antibody against human carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM8) recombinant protein (lane 2). Expected molecular weight - 13.03 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-CEACAM8-2 Western Blot Cell Lysate
Result: Positive
Western blot using CPTC-CEACAM8-2 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), H226 (lane 6), and PBMC (lane 7) whole cell lysates. HeLa and H226 are positive. All other cell lines are negative. Expected molecular weight - 38.2 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-CEACAM8-2 Automated Western Blot Cell Lysate
Result: Negative
Automated western blot using CPTC-CEACAM8-2 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), H226 (lane 6), and PBMC (lane 7) whole cell lysates. Expected molecular weight - 38.2 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Background
NCI Identification Number:
00471
Antigen Name:
CEA Cell Adhesion Molecule 8 Peptide 1
CPTC Name:
CPTC-CEACAM8 Peptide 1
Aliases:
CEA Cell Adhesion Molecule 8; Carcinoembryonic Antigen-Related Cell Adhesion Molecule 8; Carcinoembryonic Antigen Related Cell Adhesion Molecule 8; Non-Specific Cross-Reacting Antigen NCA-95; Carcinoembryonic Antigen CGM6; CD67 Antigen; CD66b; CGM6; Carcinoembryonic Antigen Gene Family Member 6; CD66b Antigen; CEACAM8; NCA-95; CD67
Function:
CEACAM8 (CEA Cell Adhesion Molecule 8) is a Protein Coding gene. Diseases associated with CEACAM8 include Hemoglobinuria and Paroxysmal Nocturnal Hemoglobinuria. Among its related pathways are Degradation of the extracellular matrix and Cell surface interactions at the vascular wall. An important paralog of this gene is CEACAM6.Cell surface glycoprotein that plays a role in cell adhesion in a calcium-independent manner (PubMed:8776764, PubMed:2022629, PubMed:11590190). Mediates heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed:8776764, PubMed:2022629, PubMed:11590190). Heterophilic interaction with CEACAM8 occurs in activated neutrophils.
Chromosomal Localization:
19q13.2
Accession Number:
NP_001807.2
UniProt Accession Number:
P31997
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
IIGYVISNQQITPGPAYSNR
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
2200
Last Updated:
12/16/2020
Links
- EnsEMBL - EnsEMBL CEACAM8
- Entrez - Entrez CEACAM8
- Uniprot - Uniprot P31997
Characterization Data
SOPs
No SOPs available.
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