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Cyclin Dependent Kinase 7 Peptide 1

Antibody: CPTC-CDK7-1
Background

Catalog Number:

CPTC-CDK7-1

Target Antigen:

Cyclin Dependent Kinase 7 Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous, Phosphorylation

External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (284.8 KB)

CPTC-CDK7-1 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC NCI60
Click to enlarge image Immuno-histochemistry of CPTC-CDK7-1 for NCI60 Cell Line Array with titer 1:1250 0=NEGATIVE 1=WEAK(red) 2=MODERATE(blue) 3=STRONG(green) Click image to enlarge

CPTC-CDK7-1 IHC NCI60

Result: Positive

Immuno-histochemistry of CPTC-CDK7-1 for NCI60 Cell Line Array with titer 1:1250
0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green)


Characterization SOP Files

  IHC Tissue
Download Tissue Micro-Array(TMA) core of pancreatic cancer showing nuclear staining using Antibody CPTC-CDK7-1. Titer: 1:1250 (2.6 MB)

CPTC-CDK7-1 IHC Tissue

Result: Positive

Tissue Micro-Array(TMA) core of pancreatic cancer showing nuclear staining using Antibody CPTC-CDK7-1. Titer: 1:1250


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-CDK7-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5912) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysate. Click image to enlarge

CPTC-CDK7-1 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-CDK7-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5912)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysate.


Characterization SOP Files

  Immunofluorescence
Click to enlarge image Immunofluorescence staining of human cell line HeLa with CPTC-CDK7-1 Ab shows localization to the nucleus, other. Click image to enlarge

CPTC-CDK7-1

Result: Positive

Immunofluorescence staining of human cell line HeLa with CPTC-CDK7-1 Ab shows localization to the nucleus, other.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-CDK7-1 as primary antibody against CDK7 recombinant protein (lane 2). Also included are molecular weight standards (lane 1) Click image to enlarge

CPTC-CDK7-1 western blot (recombinant protein)

Result: Positive

Western Blot using CPTC-CDK7-1 as primary antibody against CDK7 recombinant protein (lane 2). Also included are molecular weight standards (lane 1)


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-CDK7-1 as primary antibody against cell lysates LCL57 (lane 2), HeLa (lane 3) and MCF10A (lane 4). Also included are molecular weight standards (lane 1) Click image to enlarge

CPTC-CDK7-1 western blot (cell lysates)

Result: Positive

Western Blot using CPTC-CDK7-1 as primary antibody against cell lysates LCL57 (lane 2), HeLa (lane 3) and MCF10A (lane 4). Also included are molecular weight standards (lane 1)


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western Blot using CPTC-CDK-1 as primary antibody against cell lysates LCL57 (lane 2), HeLa (lane 3) and MCF10A (lane 4). Also included are molecular weight standards (lane 1) Click image to enlarge

CPTC-CDK-1 automated western blot (cell lysates)

Result: Positive

Automated western Blot using CPTC-CDK-1 as primary antibody against cell lysates LCL57 (lane 2), HeLa (lane 3) and MCF10A (lane 4). Also included are molecular weight standards (lane 1)


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Single cell western blot using CPTC-CDK7-1 as a primary antibody against cell lysates. Relative expression of total CDK7 in HeLa, MCF10A, and LCL57 cells (A). Percentage of cells that express CDK7 (B). Average expression of CDK7 protein per cell (C). All data is normalized to β-tubulin expression. Click image to enlarge

CPTC-CDK7-1 Single Cell Western Blot (Cell Lysate)

Result: Positive

Single cell western blot using CPTC-CDK7-1 as a primary antibody against cell lysates. Relative expression of total CDK7 in HeLa, MCF10A, and LCL57 cells (A). Percentage of cells that express CDK7 (B). Average expression of CDK7 protein per cell (C). All data is normalized to β-tubulin expression.


Characterization SOP Files

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Antigen: Cyclin Dependent Kinase 7 Peptide 1
Background

NCI Identification Number:

00312

Antigen Name:

Cyclin Dependent Kinase 7 Peptide 1

CPTC Name:

CPTC-CDK7 Peptide 1

Aliases:

Cyclin Dependent Kinase 7; Cyclin-Dependent Kinase 7 (MO15 Homolog, Xenopus Laevis, Cdk-Activating Kinase); TFIIH Basal Transcription Factor Complex Kinase Subunit; Cell Division Protein Kinase 7; Cyclin-Dependent Kinase 7; CDK-Activating Kinase 1; 39 KDa Protein Kinase; EC 2.7.11.22; CDKN7; CAK1; MO15; STK1; CAK; Homolog Of Xenopus MO15 Cdk-Activating Kinase; Serine/Threonine Protein Kinase MO15; Serine/Threonine Protein Kinase 1; Serine/Threonine-Protein Kinase 1; Serine/Threonine Kinase Stk1; Kinase Subunit Of CAK; EC 2.7.11.24; EC 2.7.11.23; EC 2.7.11; P39 Mo15; P39MO15; HCAK

Function:

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle. CDK7 (Cyclin Dependent Kinase 7) is a Protein Coding gene. Diseases associated with CDK7 include Xeroderma Pigmentosum, Variant Type and Hemophagocytic Lymphohistiocytosis, Familial, 3. Among its related pathways are Formation of HIV-1 elongation complex containing HIV-1 Tat and RNA Polymerase II Transcription Initiation And Promoter Clearance. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is CDK3.Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of CDK1/cyclin-B during G2-M transition, and for activation of CDK2/cyclins during G1-S transition (but not complex formation). CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Phosphorylation of POLR2A in complex with DNA promotes transcription initiation by triggering dissociation from DNA. Its expression and activity are constant throughout the cell cycle. Upon DNA damage, triggers p53/TP53 activation by phosphorylation, but is inactivated in turn by p53/TP53; this feedback loop may lead to an arrest of the cell cycle and of the transcription, helping in cell recovery, or to apoptosis. Required for DNA-bound peptides-mediated transcription and cellular growth inhibition.

Chromosomal Localization:

5q13.2

Accession Number:

NP_001790.1

UniProt Accession Number:

P50613

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

CAYT(ph)HQVVTR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1100

Last Updated:

08/21/2020

Links

Characterization Data

SOPs

No SOPs available.

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