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Cell Division Cycle 25C Peptide 1

Antibody: CPTC-CDC25C-1
Background

Catalog Number:

CPTC-CDC25C-1

Target Antigen:

Cell Division Cycle 25C Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Endogenous, Phosphorylation

External Links
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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (331.1 KB)

CPTC-CDC25C-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC NCI60
Click to enlarge image Immuno-histochemistry of CPTC-CDC25C-1 for NCI60 Cell Line Array with titer 1:50 0=NEGATIVE 1=WEAK(red) 2=MODERATE(blue) 3=STRONG(green) Click image to enlarge

CPTC-CDC25C-1 IHC NCI60

Result: Positive

Immuno-histochemistry of CPTC-CDC25C-1 for NCI60 Cell Line Array with titer 1:50
0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green)


Characterization SOP Files

  IHC Tissue
Download Tissue Micro-Array(TMA) core of pancreatic cancer showing cytoplasmic staining using Antibody CPTC-CDC25C-1. Titer: 1:50 (2.8 MB)

CPTC-CDC25C-1 IHC Tissue

Result: Positive

Tissue Micro-Array(TMA) core of pancreatic cancer showing cytoplasmic staining using Antibody CPTC-CDC25C-1. Titer: 1:50


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-CDC25C-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5906) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysate. Click image to enlarge

CPTC-CDC25C-1 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-CDC25C-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5906)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysate.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-CDC25C-1 as primary antibody against cell lysates HeLa, MCF10A, and LCL57. Samples from each cell line were irradiated with 10 Gy as shown in the ‘+’ indicated lanes. Samples from each non-irradiated cell line were treated with alkaline phosphatase enzyme as shown in the ‘-‘ indicated lanes. Molecular weight standards are also included for each cell line. Click image to enlarge

CPTC-CDC25C-1 automated western blot (cell lysate)

Result: Negative

Automated western blot using CPTC-CDC25C-1 as primary antibody against cell lysates HeLa, MCF10A, and LCL57. Samples from each cell line were irradiated with 10 Gy as shown in the ‘+’ indicated lanes. Samples from each non-irradiated cell line were treated with alkaline phosphatase enzyme as shown in the ‘-‘ indicated lanes. Molecular weight standards are also included for each cell line.


Characterization SOP Files

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Antigen: Cell Division Cycle 25C Peptide 1
Background

NCI Identification Number:

00310

Antigen Name:

Cell Division Cycle 25C Peptide 1

CPTC Name:

CPTC-CDC25C Peptide 1

Aliases:

Cell Division Cycle 25C; Protein Phosphatase 1, Regulatory Subunit 60; M-Phase Inducer Phosphatase 3; EC 3.1.3.48; CDC25; Dual Specificity Phosphatase CDC25C; Dual Specificity Phosphatase Cdc25C; Phosphotyrosine Phosphatase; Mitosis Inducer CDC25; PPP1R60

Function:

This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described.CDC25C (Cell Division Cycle 25C) is a Protein Coding gene. Diseases associated with CDC25C include Plague and Prostate Cancer. Among its related pathways are Human cytomegalovirus infection and Oocyte meiosis. Gene Ontology (GO) annotations related to this gene include protein kinase binding and WW domain binding. An important paralog of this gene is CDC25B.Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.Cdc25 is a dual specificity phosphatase with three isoforms in mammalian cells - Cdc25A, B and C. Cdc25 activates cdk complexes that drive the cell cycle. Cdc25 is involved in the DNA damage checkpoints and is known as a key mediator of cell cycle progression.

Chromosomal Localization:

5q31.2

Accession Number:

NP_001781.2

UniProt Accession Number:

P30307

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

CSPS(ph)MPENLNRPR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1430

Last Updated:

08/21/2020

Links

Characterization Data

SOPs

No SOPs available.

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