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CD70 Molecule Peptide 1

Antibody: CPTC-CD70-1
Background

Catalog Number:

CPTC-CD70-1

Target Antigen:

CD70 Molecule Peptide 1

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Endogenous

Thematic Panel(s):

Immuno-Oncology (IO)

External Links
Publications
Characterization Data [Compare Characterization Data]
  IHC Tissue

CPTC-CD70-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-CD70-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5959) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool. Click image to enlarge

CPTC-CD70-1 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-CD70-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5959)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool.


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-CD70-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-CD70-1 NCI 60 Protein Array

Result: Positive

Protein Array in which CPTC-CD70-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide

CPTC-CD70-1 Western Blot Recombinant Protein

Result: Negative

Western blot using CPTC-CD70-1 as primary antibody against human CD70 molecule (CD70) recombinant protein (lane 2). Expected molecular weight - 20.9 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-CD70-1 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), H226 (lane 6), and PBMC (lane 7) whole cell lysates. Expected molecular weight - 21.1 kDa and 23.4 kDa. Molecular weight standards are also included (lane 1). A549, HeLa and MCF7 are positive. All other cell lines are negative. Target protein is subject to glycosylation which can affect the migration in electrophoresis. This can make the target appear as a higher molecular weight protein. Click image to enlarge

CPTC-CD70-1 Western Blot Cell Lysate

Result: Positive

Western blot using CPTC-CD70-1 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), H226 (lane 6), and PBMC (lane 7) whole cell lysates. Expected molecular weight - 21.1 kDa and 23.4 kDa. Molecular weight standards are also included (lane 1). A549, HeLa and MCF7 are positive. All other cell lines are negative. Target protein is subject to glycosylation which can affect the migration in electrophoresis. This can make the target appear as a higher molecular weight protein.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-CD70-1 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), H226 (lane 6), and PBMC (lane 7) whole cell lysates. Expected molecular weight - 21.1 kDa and 23.4 kDa. Molecular weight standards are also included (lane 1). A549 is positive. All other cell lines are negative. Target protein is subject to glycosylation which can affect the migration in electrophoresis. This can make the target appear as a higher molecular weight protein. Click image to enlarge

CPTC-CD70-1 Automated Western Blot Cell Lysate

Result: Positive

Automated western blot using CPTC-CD70-1 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), H226 (lane 6), and PBMC (lane 7) whole cell lysates. Expected molecular weight - 21.1 kDa and 23.4 kDa. Molecular weight standards are also included (lane 1). A549 is positive. All other cell lines are negative. Target protein is subject to glycosylation which can affect the migration in electrophoresis. This can make the target appear as a higher molecular weight protein.


Characterization SOP Files

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Antibody: CPTC-CD70-2
Background

Catalog Number:

CPTC-CD70-2

Target Antigen:

CD70 Molecule Peptide 1

Isotype:

IgG

Species:

Mouse Monoclonal Antibody

Last Updated:

02/02/2024

Antigen Recognition(s):

Peptide

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Characterization Data [Compare Characterization Data]
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Antibody: CPTC-CD70-3
Background

Catalog Number:

CPTC-CD70-3

Target Antigen:

CD70 Molecule Peptide 1

Isotype:

IgG

Species:

Mouse Monoclonal Antibody

Last Updated:

02/02/2024

Antigen Recognition(s):

Peptide

Purchase
Characterization Data [Compare Characterization Data]
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Antigen: CD70 Molecule Peptide 1
Background

NCI Identification Number:

00470

Antigen Name:

CD70 Molecule Peptide 1

CPTC Name:

CPTC-CD70 Peptide 1

Aliases:

CD70 Molecule; CD27L; Tumor Necrosis Factor Ligand Superfamily Member 7; CD70 Antigen; CD27 Ligand; CD27-L; CD27LG; TNFSF7; Tumor Necrosis Factor (Ligand) Superfamily, Member 7; Tumor Necrosis Factor Ligand 8A; Surface Antigen CD70; Ki-24 Antigen; TNLG8A; LPFS3; CD70

Function:

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin sythesis. CD70 (CD70 Molecule) is a Protein Coding gene. Diseases associated with CD70 include Lymphoproliferative Syndrome 3 and Acute Myocarditis. Among its related pathways are PEDF Induced Signaling and TNF Superfamily - Human Ligand-Receptor Interactions and their Associated Functions. Gene Ontology (GO) annotations related to this gene include signaling receptor binding and protease binding.Cytokine which is the ligand for CD27. The CD70-CD27 pathway plays an important role in the generation and maintenance of T cell immunity, in particular during antiviral responses. Upon CD27 binding, induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.

Chromosomal Localization:

19p13.3

Accession Number:

NP_001243.1

UniProt Accession Number:

P32970

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

LYWQGGPALGR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1210

Last Updated:

12/16/2020

Links

Characterization Data

SOPs

No SOPs available.

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