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CD33 Molecule Peptide 1

Antibody: CPTC-CD33-1
Background

Catalog Number:

CPTC-CD33-1

RRID:

AB_2884036

Target Antigen:

CD33 Molecule Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Endogenous

Thematic Panel(s):

Immuno-Oncology (IO)

External Links
Purchase
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (226.4 KB)

CPTC-CD33-1Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of lung cancer showing cytoplasmic and membranous staining using Antibody CPTC-CD33-1. Titer: 1:250 Click image to enlarge

CTPC-CD33-1 IHC Tissue

Result: Positive

Tissue Micro-Array (TMA) core of lung cancer showing cytoplasmic and membranous staining using Antibody CPTC-CD33-1. Titer: 1:250


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-CD33-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5951) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool. Click image to enlarge

CPTC-CD33-1 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-CD33-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5951)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool.


Characterization SOP Files

  NCI 60 Protein Array

CTPC-CD33-1 NCI60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-CD33-1 as primary antibody against human CD33 recombinant protein (lane 2). Expected molecular weight - 38 kDa. Molecular weight standards are also included (lane 1). Inconclusive data. Click image to enlarge

CPTC-CD33-1 Western Blot (recombinant protein)

Result: Presumed Positive (with additional bands)

Western blot using CPTC-CD33-1 as primary antibody against human CD33 recombinant protein (lane 2). Expected molecular weight - 38 kDa.
Molecular weight standards are also included (lane 1). Inconclusive data.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-CD33-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) cell lysates. Expected molecular weight - 38 kDa. Molecular weight standards are also included (lane 1). Inconclusive data. Click image to enlarge

CPTC-CD33-1 Automated Western Blot (Cell Lysate)

Result: Negative

Automated western blot using CPTC-CD33-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) cell lysates. Expected molecular weight - 38 kDa. Molecular weight standards are also included (lane 1). Inconclusive data.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-CD33-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5) and NCI H226 (lane 6) cell lysates. Expected molecular weight 40 kDa. Molecular weight standards (MW Stds.) are also included (lane 1). Positive for cell lines Jurkat and MCF7. Negative/inconclusive data for the other cell lines. Click image to enlarge

CPTC-CD33-1 Western Blot (Cell Lysate)

Result: Positive

Western blot using CPTC-CD33-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5) and NCI H226 (lane 6) cell lysates. Expected molecular weight 40 kDa. Molecular weight standards (MW Stds.) are also included (lane 1). Positive for cell lines Jurkat and MCF7. Negative/inconclusive data for the other cell lines.


Characterization SOP Files

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Antibody: CPTC-CD33-2
Background

Catalog Number:

CPTC-CD33-2

Target Antigen:

CD33 Molecule Peptide 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Endogenous

Characterization Data [Compare Characterization Data]
  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-CD33-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-CD33-2 NCI 60 Protein Array

Result: Positive

Protein Array in which CPTC-CD33-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

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Antibody: CPTC-CD33-3
Background

Catalog Number:

CPTC-CD33-3

Target Antigen:

CD33 Molecule Peptide 1

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

02/02/2024

Antigen Recognition(s):

Peptide

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Characterization Data [Compare Characterization Data]
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Antibody: CPTC-CD33-4
Background

Catalog Number:

CPTC-CD33-4

Target Antigen:

CD33 Molecule Peptide 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

02/02/2024

Antigen Recognition(s):

Peptide

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Characterization Data [Compare Characterization Data]
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Antigen: CD33 Molecule Peptide 1
Background

NCI Identification Number:

00352

Antigen Name:

CD33 Molecule Peptide 1

CPTC Name:

CPTC-CD33 Peptide 1

Aliases:

CD33 Molecule; SIGLEC3; Sialic Acid-Binding Ig-Like Lectin 3; Myeloid Cell Surface Antigen CD33; CD33 Antigen (Gp67); SIGLEC-3; Gp67; P67; Sialic Acid Binding Ig-Like Lectin 3; CD33 Molecule Transcript; CD33 Antigen; FLJ00391; Siglec-3; CD33

Function:

CD33 (CD33 Molecule) is a Protein Coding gene. Diseases associated with CD33 include Acute Leukemia and Acute Promyelocytic Leukemia. Among its related pathways are Hematopoietic cell lineage and Hematopoietic Stem Cells and Lineage-specific Markers. Gene Ontology (GO) annotations related to this gene include carbohydrate binding. An important paralog of this gene is SIGLEC6.ialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (PubMed:10611343, PubMed:15597323, PubMed:11320212). Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans (PubMed:7718872). Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK (PubMed:28325905, PubMed:10887109). These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:10556798, PubMed:10206955, PubMed:10887109). In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules (PubMed:10206955, PubMed:10887109). One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K (PubMed:15597323).

Chromosomal Localization:

19q13.41

Accession Number:

NP_001763.3

UniProt Accession Number:

P20138

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

ILIPGTLEPGHSK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1430

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

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