Catalog Number:
CPTC-CCL5-1
Target Antigen:
C-C Motif Chemokine Ligand 5 Peptide 1
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
03/01/2024
Antigen Recognition(s):
Peptide
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Catalog Number:
CPTC-CCL5-2
Target Antigen:
C-C Motif Chemokine Ligand 5 Peptide 1
Isotype:
IgG3
Species:
Mouse Monoclonal Antibody
Last Updated:
03/20/2024
Antigen Recognition(s):
Peptide, Endogenous
Thematic Panel(s):
Immuno-Oncology (IO)
Result: Positive
"Immuno-MRM chromatogram of CPTC-CCL5-2 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5948)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html).
Data shown were obtained from FFPE tumor tissue lysate pool.
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Result: Negative
Western blot using CPTC-CCL5-2 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), H226 (lane 6), and PBMC (lane 7) whole cell lysates. Expected molecular weight - 10 kDa. Molecular weight standards are also included (lane 1).
Result: Negative
Automated western blot using CPTC-CCL5-2 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), H226 (lane 6), and PBMC (lane 7) whole cell lysates. Expected molecular weight - 10 kDa. Molecular weight standards are also included (lane 1).
NCI Identification Number:
00466
Antigen Name:
C-C Motif Chemokine Ligand 5 Peptide 1
CPTC Name:
CPTC-CCL5 Peptide 1
Aliases:
C-C Motif Chemokine Ligand 5; SIS-Delta; TCP228; Regulated Upon Activation, Normally T-Expressed, And Presumably Secreted; Small Inducible Cytokine Subfamily A (Cys-Cys), Member 5; Eosinophil Chemotactic Cytokine; Chemokine (C-C Motif) Ligand 5; T-Cell Specific Protein P288; Beta-Chemokine RANTES; C-C Motif Chemokine 5; D17S136E; RANTES; SCYA5; SISd; EoCP; Small Inducible Cytokine A5 (RANTES); T-Cell Specific RANTES Protein; T-Cell-Specific Protein RANTES; T Cell-Specific Protein P228; Small-Inducible Cytokine A5; MGC17164; CCL5
Function:
This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. CCL5 (C-C Motif Chemokine Ligand 5) is a Protein Coding gene. Diseases associated with CCL5 include Periapical Granuloma and Ulcer Of Lower Limbs. Among its related pathways are Signaling by GPCR and Validated targets of C-MYC transcriptional repression. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and chemokine activity. An important paralog of this gene is CCL3L1.Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils (PubMed:16791620, PubMed:1380064, PubMed:8525373, PubMed:9516414, PubMed:15923218). May also be an agonist of the G protein-coupled receptor GPR75, stimulating inositol trisphosphate production and calcium mobilization through its activation. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.
Chromosomal Localization:
17q12
Accession Number:
NP_002976.2
UniProt Accession Number:
P13501
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
EYFYTSGK
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
880
Last Updated:
12/16/2020
No SOPs available.
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