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Calreticulin Peptide 1

Antibody: CPTC-CALR-1
Background

Catalog Number:

CPTC-CALR-1

RRID:

AB_2617228

Target Antigen:

Calreticulin Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

02/26/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (618.0 KB)

CPTC-CALR-1 evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Immuno-MRM
Click to enlarge image "Immuno-MRM chromatogram of CPTC-CALR-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-698) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)" Click image to enlarge

CPTC-CALR-1 iMRM

Result: Positive

"Immuno-MRM chromatogram of CPTC-CALR-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-698)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)"


Characterization SOP Files

  Immunofluorescence

CPTC-CALR-1 Immunofluorescence

Result: Negative

Immunofluorescence staining of MCF7 cells using CPTC-CALR-1 antibody. CALR protein expression was not detected.


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (618.0 KB)

CPTC-CALR-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-CALR-1 peptide ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to full-length Antigen coated on plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-CALR-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to full-length Antigen coated on plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-CALR-1 as primary Ab against HEK293T cell lysate containing CALR (from Origene) in lane 2. Also included are molecular wt. standards (lane 1) and the CALR-1 Ab as the IgG control (lane 3). Click image to enlarge

CPTC-CALR-1 Western blot (cell lysate)

Result: Positive

Western Blot using CPTC-CALR-1 as primary Ab against HEK293T cell lysate containing CALR (from Origene) in lane 2. Also included are molecular wt. standards (lane 1) and the CALR-1 Ab as the IgG control (lane 3).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-CALR-1 as primary Ab against lysates from six breast cancer cell lines from the NCI60 cell line collection (lanes 2-7). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-CALR-1 NCI60 Western blot

Result: Positive

Western Blot using CPTC-CALR-1 as primary Ab against lysates from six breast cancer cell lines from the NCI60 cell line collection (lanes 2-7). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

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Antigen: Calreticulin Peptide 1
Background

NCI Identification Number:

00025

Antigen Name:

Calreticulin Peptide 1

CPTC Name:

CPTC-CALR Peptide 1

Aliases:

SSA; CRT; RO; CC1qR; Endoplasmic Reticulum Resident; Protein 60; CRP55; ERp60; HACBP; Grp60; FLJ26680; Calregulin; Sicca Syndrome Antigen A (Autoantigen Ro; Calreticulin); CRTC

Function:

Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.

Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis.

Chromosomal Localization:

10p13.3 - p13.2

Accession Number:

NP_004334.1

UniProt Accession Number:

P27797

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

GLQTSQDAR

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

990

Last Updated:

06/11/2013

Links

Characterization Data

SOPs

No SOPs available.

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