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BRCA1 Interacting Protein C-Terminal Helicase 1 Peptide 1

Antibody: CPTC-BRIP1-1
Background

Catalog Number:

CPTC-BRIP1-1

RRID:

AB_2617226

Target Antigen:

BRCA1 Interacting Protein C-Terminal Helicase 1 Peptide 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

11/21/2023

Antigen Recognition(s):

Peptide, Phosphorylation

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (607.5 KB)

CPTC-BRIP1-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IP
Click to enlarge image Immuno-Precipitation Mass Spectrometry using CPTC-BRIP1-1 antibody with CPTC-BRIP1 peptide 1 (phosphorylated) as the target antigen. Click image to enlarge

CPTC-BRIP1-1 phospho peptide IPMS

Result: Positive

Immuno-Precipitation Mass Spectrometry using CPTC-BRIP1-1 antibody with CPTC-BRIP1 peptide 1 (phosphorylated) as the target antigen.


Characterization SOP Files

  IP
Click to enlarge image Immuno-Precipitation Mass Spectrometry using CPTC-BRIP1-1 antibody with CPTC-BRIP1 peptide 1 (non-phosphorylated) as the target antigen. Click image to enlarge

CPTC-BRIP1-1 non-phospho peptide IPMS

Result: Positive

Immuno-Precipitation Mass Spectrometry using CPTC-BRIP1-1 antibody with CPTC-BRIP1 peptide 1 (non-phosphorylated) as the target antigen.


Characterization SOP Files

  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (607.5 KB)

CPTC-BRIP1-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-BRIP1 peptide 1-1 Indirect ELISA

Result: Positive

Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect peptide ELISA (ie, binding of Antibody to biotinylated non-phosphorylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-BRIP1-1 Indirect ELISA

Result: Positive

Indirect peptide ELISA (ie, binding of Antibody to biotinylated non-phosphorylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-BRIP1-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-BRIP1-1 as primary antibody against HT-29 (lane 2), HeLa (lane 3), MCF7 (lane 4), HL-60 (lane 5), Hep G2 (lane 6), and MCF7 (lane 7) whole cell lysates. Expected molecular weight - 141 kDa and 112 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-BRIP1-1 Western Blot

Result: Negative

Western blot using CPTC-BRIP1-1 as primary antibody against HT-29 (lane 2), HeLa (lane 3), MCF7 (lane 4), HL-60 (lane 5), Hep G2 (lane 6), and MCF7 (lane 7) whole cell lysates. Expected molecular weight - 141 kDa and 112 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-BRIP1-1 as primary antibody against HT-29 (lane 2), HeLa (lane 3), MCF7 (lane 4), HL-60 (lane 5), Hep G2 (lane 6), and MCF7 (lane 7) whole cell lysates. Expected molecular weight - 141 kDa and 112 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-BRIP1-1 Automated Western Blot Cell Lysate

Result: Negative

Automated western blot using CPTC-BRIP1-1 as primary antibody against HT-29 (lane 2), HeLa (lane 3), MCF7 (lane 4), HL-60 (lane 5), Hep G2 (lane 6), and MCF7 (lane 7) whole cell lysates. Expected molecular weight - 141 kDa and 112 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antibody: CPTC-BRIP1-2
Background

Catalog Number:

CPTC-BRIP1-2

RRID:

AB_2617227

Target Antigen:

BRCA1 Interacting Protein C-Terminal Helicase 1 Peptide 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

11/21/2023

Antigen Recognition(s):

Peptide, Phosphorylation

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (1.0 MB)

CPTC-BRIP1-2 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (1.0 MB)

CPTC-BRIP1-2 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect peptide ELISA (ie, binding of Antibody to biotinylated non-phosphorylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-BRIP1-2 Indirect ELISA

Result: Positive

Indirect peptide ELISA (ie, binding of Antibody to biotinylated non-phosphorylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-BRIP1 peptide 1-2 Indirect ELISA

Result: Positive

Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-BRIP1-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-BRIP1-2 as primary antibody against HT-29 (lane 2), HeLa (lane 3), MCF7 (lane 4), HL-60 (lane 5), Hep G2 (lane 6), and MCF7 (lane 7) whole cell lysates. Expected molecular weight - 141 kDa and 112 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-BRIP1-2 Western Blot

Result: Negative

Western blot using CPTC-BRIP1-2 as primary antibody against HT-29 (lane 2), HeLa (lane 3), MCF7 (lane 4), HL-60 (lane 5), Hep G2 (lane 6), and MCF7 (lane 7) whole cell lysates. Expected molecular weight - 141 kDa and 112 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-BRIP1-2 as primary antibody against HT-29 (lane 2), HeLa (lane 3), MCF7 (lane 4), HL-60 (lane 5), Hep G2 (lane 6), and MCF7 (lane 7) whole cell lysates. Expected molecular weight - 141 kDa and 112 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-BRIP1-2 Automated Western Blot Cell Lysate

Result: Negative

Automated western blot using CPTC-BRIP1-2 as primary antibody against HT-29 (lane 2), HeLa (lane 3), MCF7 (lane 4), HL-60 (lane 5), Hep G2 (lane 6), and MCF7 (lane 7) whole cell lysates. Expected molecular weight - 141 kDa and 112 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antibody: CPTC-BRIP1-3
Background

Catalog Number:

CPTC-BRIP1-3

RRID:

AB_2820259

Target Antigen:

BRCA1 Interacting Protein C-Terminal Helicase 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

11/21/2023

Antigen Recognition(s):

Peptide, Phosphorylation

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

External Links
Purchase
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (553.4 KB)

CPTC-BRIP1-3 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-BRIP1-3 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3222) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html) Click image to enlarge

CPTC-BRIP1-3 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-BRIP1-3 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3222)

Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)


Characterization SOP Files

  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (553.4 KB)

CPTC-BRIP1-3 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  NCI 60 Protein Array

CPTC-BRIP1-3 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-BRIP1-3 as primary antibody against HT-29 (lane 2), HeLa (lane 3), MCF7 (lane 4), HL-60 (lane 5), Hep G2 (lane 6), and MCF7 (lane 7) whole cell lysates. Expected molecular weight - 141 kDa and 112 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-BRIP1-3 Western Blot

Result: Negative

Western blot using CPTC-BRIP1-3 as primary antibody against HT-29 (lane 2), HeLa (lane 3), MCF7 (lane 4), HL-60 (lane 5), Hep G2 (lane 6), and MCF7 (lane 7) whole cell lysates. Expected molecular weight - 141 kDa and 112 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-BRIP1-3 as primary antibody against HT-29 (lane 2), HeLa (lane 3), MCF7 (lane 4), HL-60 (lane 5), Hep G2 (lane 6), and MCF7 (lane 7) whole cell lysates. Expected molecular weight - 141 kDa and 112 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-BRIP1-3 Automated Western Blot Cell Lysate

Result: Negative

Automated western blot using CPTC-BRIP1-3 as primary antibody against HT-29 (lane 2), HeLa (lane 3), MCF7 (lane 4), HL-60 (lane 5), Hep G2 (lane 6), and MCF7 (lane 7) whole cell lysates. Expected molecular weight - 141 kDa and 112 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antibody: CPTC-BRIP1-4
Background

Catalog Number:

CPTC-BRIP1-4

RRID:

AB_2820260

Target Antigen:

BRCA1 Interacting Protein C-Terminal Helicase 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

11/21/2023

Antigen Recognition(s):

Peptide, Endogenous, Phosphorylation

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

External Links
Purchase
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (645.9 KB)

CPTC-BRIP1-4 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-BRIP1-4 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3221) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html) Click image to enlarge

CPTC-BRIP1-4 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-BRIP1-4 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3221)

Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)


Characterization SOP Files

  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (645.9 KB)

CPTC-BRIP1-4 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  NCI 60 Protein Array

CPTC-BRIP1-4 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-BRIP1-4 as primary antibody against HT-29 (lane 2), HeLa (lane 3), MCF7 (lane 4), HL-60 (lane 5), Hep G2 (lane 6), and MCF7 (lane 7) whole cell lysates. Expected molecular weight - 141 kDa and 112 kDa. Molecular weight standards are also included (lane 1). HeLa and MCF10A are positive. The remaining cell lines are negative. Click image to enlarge

CPTC-BRIP1-4 Western Blot

Result: Positive

Western blot using CPTC-BRIP1-4 as primary antibody against HT-29 (lane 2), HeLa (lane 3), MCF7 (lane 4), HL-60 (lane 5), Hep G2 (lane 6), and MCF7 (lane 7) whole cell lysates. Expected molecular weight - 141 kDa and 112 kDa. Molecular weight standards are also included (lane 1). HeLa and MCF10A are positive. The remaining cell lines are negative.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-BRIP1-4 as primary antibody against HT-29 (lane 2), HeLa (lane 3), MCF7 (lane 4), HL-60 (lane 5), Hep G2 (lane 6), and MCF7 (lane 7) whole cell lysates. Expected molecular weight - 141 kDa and 112 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-BRIP1-4 Automated Western Blot Cell Lysate

Result: Positive

Automated western blot using CPTC-BRIP1-4 as primary antibody against HT-29 (lane 2), HeLa (lane 3), MCF7 (lane 4), HL-60 (lane 5), Hep G2 (lane 6), and MCF7 (lane 7) whole cell lysates. Expected molecular weight - 141 kDa and 112 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antigen: BRCA1 Interacting Protein C-Terminal Helicase 1 Peptide 1
Background

NCI Identification Number:

00086

Antigen Name:

BRCA1 Interacting Protein C-Terminal Helicase 1 Peptide 1

CPTC Name:

CPTC-BRIP1 Peptide 1

Aliases:

BRCA1 Interacting Protein C-Terminal Helicase 1; BACH1; FANCJ; BRCA1/BRCA2-Associated Helicase 1; ATP-Dependent RNA Helicase BRIP1; BRCA1-Associated C-Terminal Helicase 1; OF; BRCA1-Binding Helicase-Like Protein; Fanconi Anemia Group J Protein; EC 3.6.4.13; BRCA1-Interacting Protein 1; RCA1-Interacting Protein C-Terminal Helicase 1; Protein FACJ; EC 3.6.1

Function:

The protein encoded by this gene is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). This gene may be a target of germline cancer-inducing mutations.

BRIP1 (BRCA1 interacting protein C-terminal helicase 1) is a protein-coding gene. Diseases associated with BRIP1 include brip1-related fanconi anemia, and brip1-related breast cancer. GO annotations related to this gene include ATP-dependent DNA helicase activity and 4 iron, 4 sulfur cluster binding. An important paralog of this gene is DDX11.

DNA-dependent ATPase and 5' to 3' DNA helicase required for the maintenance of chromosomal stability. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1.

Chromosomal Localization:

17q22.2

Accession Number:

NP_114432.2

UniProt Accession Number:

Q9BX63

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

ATPELGSSENSAS(pS)PPR

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1980

Last Updated:

09/01/2020

Links

Characterization Data

SOPs

No SOPs available.

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