Background
Catalog Number:
CPTC-ATM-1
RRID:
AB_2877653
Target Antigen:
Ataxia Telangiectasia Mutated Peptide 1
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
02/26/2024
Antigen Recognition(s):
Peptide, Endogenous, Phosphorylation
Thematic Panel(s):
DNA Damage Response (DDR) Pathway
External Links
- CPTAC Assay Portal CPTAC-3211
Purchase
- DSHB CPTC-ATM-1
Publications
- A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage (PMID: 29474155)
- Integrated Proteogenomic Characterization across Major Histological Types of Pediatric Brain Cancer (PMID: 33242424)
Characterization Data [Compare Characterization Data]
Immuno-MRM
CPTC-ATM-1 iMRM
Result: Positive
Immuno-MRM chromatogram of CPTC-ATM-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3211)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)
Background
Catalog Number:
CPTC-ATM-2
Target Antigen:
Ataxia Telangiectasia Mutated Peptide 1
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
09/06/2023
Antigen Recognition(s):
Peptide, Phosphorylation
Thematic Panel(s):
DNA Damage Response (DDR) Pathway, Immuno-Oncology (IO)
External Links
- CPTAC Assay Portal CPTAC-3210
- CPTAC Assay Portal CPTAC-5976
- CPTAC Assay Portal CPTAC-5977 (Phospho)
Publications
- A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage (PMID: 29474155)
- Targeted Mass Spectrometry Enables Multiplexed Quantification of Immunomodulatory Proteins in Clinical Biospecimens (PMID: 34858420)
Characterization Data [Compare Characterization Data]
Immuno-MRM
CPTC-ATM-2 iMRM
Result: Positive
Immuno-MRM chromatogram of CPTC-ATM-2 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3210)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)
Characterization SOP Files
Background
NCI Identification Number:
00101
Antigen Name:
Ataxia Telangiectasia Mutated Peptide 1
CPTC Name:
CPTC-ATM Peptide 1
Aliases:
ATA; ATC; ATD; ATDC; Tel1; A-T Mutated; AT1; Ataxia Telangiectasia Mutated; EC 2.7.11.1; Telomere Maintenance 1; Ataxia Telangiectasia Mutated (Includes Complementation Groups A, C And D); ATE; TELO1; AT mutated; Serine-Protein Kinase ATM; TEL1, Telomere Maintenance 1, Homolog
Function:
The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle
checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder.
ATM (ataxia telangiectasia mutated) is a protein-coding gene. Diseases associated with ATM include chromosome 11q deletion, and ataxia telangiectasia. GO annotations related to this gene include protein serine/threonine kinase activity and protein complex binding.
Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual
B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates DYRK2, CHEK2, p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends. Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation. Phosphorylates ATF2 which stimulates its function in DNA damage response.
ATM (Ataxia telangiectasia mutated) and ATR (Ataxia telangiectasia and Rad3 related) are closely related kinases that are activated by DNA damage. These serine-threonine protein kinases are part of the phosphatidylinositol 3' kinase-like kinase (PIKK) family. Upon recruitment by the DNA damage binding proteins/complexes (ATRIP for ATR; MRN for ATM), ATM/ATR initiate the DNA damage checkpoint by phosphorylating a number of key proteins. Once activated, the checkpoint leads to cell cycle arrest and either DNA repair or apoptosis. ATM is activated by double stranded breaks and phosphorylates Chk2, whilst ATR is activated by single strand breaks and phosphorylates Chk1.
Chromosomal Localization:
11q22.q23
Accession Number:
NP_000042.3
UniProt Accession Number:
Q13315
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
SLEI(pS)QSYTTTQR (pS367)
Native Sequence:
Calculated Isoelectric Point:
0
Molecular Weight:
1430
Last Updated:
03/11/2015
Links
- EnsEMBL - EnsEMBL ATM
- Entrez - Entrez ATM
- Uniprot - Uniprot Q13315
Characterization Data
SOPs
No SOPs available.
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