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Annexin A1 Peptide 2

Antibody: CPTC-ANXA1-5
Background

Catalog Number:

CPTC-ANXA1-5

Target Antigen:

Annexin A1 Peptide 2

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

02/26/2024

Antigen Recognition(s):

Peptide, Endogenous

Thematic Panel(s):

Immuno-Oncology (IO)

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (612.9 KB)

CPTC-ANXA1-5 evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Immuno-MRM
Click to enlarge image "Immuno-MRM chromatogram of CPTC-ANXA1-5 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5939) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)" Click image to enlarge

CPTC-ANXA1-5 iMRM

Result: Positive

"Immuno-MRM chromatogram of CPTC-ANXA1-5 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5939)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)"


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (612.9 KB)

CPTC-ANXA1-5 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-ANXA1-5 peptide ELISA

Result: Positive

Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Western Blot - HPA - tissue or cell lysate
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). Single band corresponding to the predicted size in kDa (+/-20%). Analysis performed using a standard panel of samples. Antibody dilution: 1:500 Click image to enlarge

CPTC-ANXA1-5 evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org).

Single band corresponding to the predicted size in kDa (+/-20%).
Analysis performed using a standard panel of samples. Antibody dilution: 1:500


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide

CPTC-ANXA1-5 Western Blot (Recombinant Protein)

Result: Negative

Western blot using CPTC-ANXA1-5 as primary antibody against human Annexin A1 (ANXA1) recombinant protein (lane 2). Molecular weight standards are also included (lane 1). Expected molecular weight – 38.5 kDa.


Characterization SOP Files

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Antigen: Annexin A1 Peptide 2
Background

NCI Identification Number:

00023

Antigen Name:

Annexin A1 Peptide 2

CPTC Name:

CPTC-ANXA1 Peptide 2

Aliases:

ANXA1; ANX1; Annexin-1; Chromobindin-9; LPC1; p35; Calpactin II; Lipocortin I; Phospholipase A2 Inhibitory Protein; P35; Calpactin-2

Function:

Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity.

Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.

Chromosomal Localization:

9q21.13

Accession Number:

BC001275

UniProt Accession Number:

P04083

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

GVDEATIIDILTK

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1430

Last Updated:

06/11/2013

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Characterization Data

SOPs

No SOPs available.

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