ubiquitin-conjugating enzyme E2, J1


Background

Catalog Number:

CPTC-UBE2J1-1

RRID:

AB_2617375

Target Antigen:

ubiquitin-conjugating enzyme E2, J1

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

07/28/2021

Antigen Recognition(s):

Recombinant Full-length

Purchase
External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (165.2 KB)

CPTC-UBE2J1-1 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-UBE2J1-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


  NCI 60 Protein Array

CPTC-UBE2J1-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-UBE2J1-1 as primary Ab against UBE2J1 (rAg 00013) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-UBE2J1-1 Western blot

Result: Positive

Western Blot using CPTC-UBE2J1-1 as primary Ab against UBE2J1 (rAg 00013) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Background

Catalog Number:

CPTC-UBE2J1-2

RRID:

AB_2617376

Target Antigen:

ubiquitin-conjugating enzyme E2, J1

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

08/25/2021

Antigen Recognition(s):

Recombinant Full-length, Endogenous

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA

CPTC-UBE2J1-2 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org)


  IHC Tissue
Click to enlarge image Tissue Micro-Array(TMA) core of breast cancer showing cytoplasmic staining using Antibody CPTC-UBE2J1-2. Titer: 1:30000 Click image to enlarge

CPTC-UBE2J1-2 IHC Tissue

Result: Positive

Tissue Micro-Array(TMA) core of breast cancer showing cytoplasmic staining using Antibody CPTC-UBE2J1-2. Titer: 1:30000


  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-UBE2J1-2 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-UBE2J1-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-UBE2J1-2 as primary Ab against UBE2J1 (rAg 00013) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-UBE2J1-2 Western blot

Result: Positive

Western Blot using CPTC-UBE2J1-2 as primary Ab against UBE2J1 (rAg 00013) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Background

Catalog Number:

CPTC-UBE2J1-3

RRID:

AB_2617377

Target Antigen:

ubiquitin-conjugating enzyme E2, J1

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

08/25/2021

Antigen Recognition(s):

Recombinant Full-length

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA

CPTC-UBE2J1-3 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org)


  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-UBE2J1-3 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-UBE2J1-3 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-UBE2J1-3 as primary Ab against UBE2J1 (rAg 00013) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-UBE2J1-3 Western blot

Result: Positive

Western Blot using CPTC-UBE2J1-3 as primary Ab against UBE2J1 (rAg 00013) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Background

NCI Identification Number:

00013

Antigen Name:

ubiquitin-conjugating enzyme E2, J1

CPTC Name:

CPTC-UBE2J1

Aliases:

NCUBE1; HSPC153; UBC6; Non-canonical ubiquitin-conjugating enzyme 1; Yeast ubiquitin-conjugating enzyme UBC6 homolog E; NCUBE-1; CGI-76; HSPC205; HSU93243; HSUBC6e; Ubc6p; ubiquitin-conjugating enzyme E2, J1, U; EC 6.3.2.19; HsUBC6e

Function:

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system.

Catalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum.

Chromosomal Localization:

6q15

Expression System:

E.coli

Accession Number:

NM_016021

UniProt Accession Number:

Q9Y385

DNA Source:

Imagenes

Immunogen:

Recombinant Full Length Protein

Vector Name:

pNIC28-Bsa4

Extinction Coefficient:

Buffers:

NaP

Expressed Sequence:

MHHHHHHSSGVDLGTENLYFQSMSPAVKRLMKEAAELKDPTDHYHAQPLE
DNLFEWHFTVRGPPDSDFDGGVYHGRIVLPPEYPMKPPSIILLTANGRFE
VGKKICLSISGHHPETWQPSWSIRTALLAIIGFMPTKGEGAIGSLDYTPE
ERRALAKKSQDFCCEGCGSAMKDVLL

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

19659

Last Updated:

04/17/2013

Links

Characterization Data

Gel

Click to enlarge image PAGE of UBE2J1 (rAg 00013) with molecular weight standards in lane 1.
Click image to enlarge

CPTC-UBE2J1 PAGE

PAGE of UBE2J1 (rAg 00013) with molecular weight standards in lane 1.

SOPs

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