Ubiquitin-conjugating enzyme E2C
Antibody: CPTC-UBE2C-1
Background
Catalog Number:
CPTC-UBE2C-1
Target Antigen:
Ubiquitin-conjugating enzyme E2C
Isotype:
IgG2b
Species:
Mouse
Last Updated:
01/24/2012
Antigen Recognition(s):
recombinant full-length
Purchase
- DSHB CPTC-UBE2C-1
External Links
- Human Protein Atlas CPTC-UBE2C-1
Characterization Data [Compare Characterization Data]
Western Blot
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CPTC-UBE2C-1 Western Blot
Western Blot using CPTC-UBE2C-1 as primary Ab against Ag 10213 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Indirect ELISA
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CPTC-UBE2C-1 Indirect ELISA
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
NCI 60 Protein Array
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CPTC-UBE2C-1 NCI60 Protein Array
Protein Array in which CPTC-UBE2C-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5.
Characterization SOP Files
IHC NCI60
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CPTC-UBE2C-1 IHC NCI60
Immuno-histochemistry of CPTC-UBE2C-1 for NCI60 Cell Line Array at titer 1:250
0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green)
Characterization SOP Files
IHC Tissue
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CPTC-UBE2C-1 IHC Tissue
Tissue Micro-Array(TMA) core of colon cancer showing cytoplasmic staining using Antibody CPTC-UBE2C-1. Titer: 1:250
Characterization SOP Files
Antigen: Ubiquitin-conjugating enzyme E2C
Background
NCI Identification Number:
10213
Antigen Name:
Ubiquitin-conjugating enzyme E2C
CPTC Name:
CPTC-UBE2C
Keywords:
UBE2C; EC 6.3.2.19; UBCH10; dJ447F3.2; OTTHUMP00000031653; OTTHUMP00000031655;
Function:
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.
Chromosomal Localization:
20q13.12
Expression System:
E. Coli
NCBI Accession Number:
NM_007019
Swiss Prot Accession Number:
O00762
DNA Source:
HIP:HsCD00001519
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
28483
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMASQNRDPAATSVAAARKGAEPSGGAARGPVGKR
LQQELMTLMMSGDKGISAFPESDNLFKWVGTIHGAAG
TVYEDLRYKLSLEFPSGYPYNAPTVKFLTPCYHPNVD
TQGNICLDILKEKWSALYDVRTILLSIQSLLGEPNID
SPLNTHAAELWKNPTAFKKYLQETYSKQVTSQEP
LQQELMTLMMSGDKGISAFPESDNLFKWVGTIHGAAG
TVYEDLRYKLSLEFPSGYPYNAPTVKFLTPCYHPNVD
TQGNICLDILKEKWSALYDVRTILLSIQSLLGEPNID
SPLNTHAAELWKNPTAFKKYLQETYSKQVTSQEP
Native Sequence:
ASQNRDPAATSVAAARKGAEPSGGAARGPVGKRLQQ
ELMTLMMSGDKGISAFPESDNLFKWVGTIHGAAGTV
YEDLRYKLSLEFPSGYPYNAPTVKFLTPCYHPNVDT
QGNICLDILKEKWSALYDVRTILLSIQSLLGEPNID
SPLNTHAAELWKNPTAFKKYLQETYSKQVTSQEP
ELMTLMMSGDKGISAFPESDNLFKWVGTIHGAAGTV
YEDLRYKLSLEFPSGYPYNAPTVKFLTPCYHPNVDT
QGNICLDILKEKWSALYDVRTILLSIQSLLGEPNID
SPLNTHAAELWKNPTAFKKYLQETYSKQVTSQEP
Calculated Isoelectric Point:
6.8
Molecular Weight:
19925
Last Updated:
11/14/2012
Links
- Uniprot - Uniprot UBE2C
- EnsEMBL - EnsEMBL UBE2C
Characterization Data
SOPs
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