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Serpin Peptidase Inhibitor, Clade A, Member 1

Antibody: CPTC-SERPINA1-1

Background

Catalog Number:
CPTC-SERPINA1-1
Target Antigen:
Serpin Peptidase Inhibitor, Clade A, Member 1
Isotype:
IgG2a
Species:
Mouse
Last Updated:
02/15/2013
Antigen Recognition(s):

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-SERPINA1-1 as primary Ab against SERPINA1 (rAg 11019) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

CPTC-SERPINA1-1 Western blot

Western Blot using CPTC-SERPINA1-1 as primary Ab against SERPINA1 (rAg 11019) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Click to enlarge

CPTC-SERPINA1-1 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.

NCI 60 Protein Array

Click to enlarge image Protein Array in which CPTC-SERPINA1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Click to enlarge

CPTC-SERPINA1-1 NCI 60 Protein Array

Protein Array in which CPTC-SERPINA1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

Antigen: Serpin Peptidase Inhibitor, Clade A, Member 1

Background

NCI Identification Number:
11019
Antigen Name:
Serpin Peptidase Inhibitor, Clade A, Member 1
CPTC Name:
CPTC-SERPINA1
Keywords:
AAT; PI; PI1; alpha1AT1; alpha-1-antitrypsin1; A1AT; A1A; Serpin A1; Alpha-1 protease inhibitor; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase,
antitrypsin), member 1; OTTHUMP00000197154; MGC23330; OTTHUMP00000197150;PRO2275; MGC9222; protease inhibitor 1 (anti-elastase), alpha-1-antitrypsin; alpha-1-antiproteinase
Function:
The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver
disease. Several transcript variants encoding the same protein have been found for this gene.

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for
plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form
inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.

Short peptide from AAT (SPAAT) is a reversible chymotrypsin inhibitor. It also inhibits elastase, but not
trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic
destruction by human leukocyte elastase (HLE).
Chromosomal Localization:
14q32.1
Expression System:
E. Coli
NCBI Accession Number:
BC015642.2
Swiss Prot Accession Number:
P01009
DNA Source:
OpenBiosystems - MHS1011-76439
Immunogen:
Recombinant Full Length Protein
Vector Name:
xx
Extinction Coefficient:
19940
Buffers:
PBS without Ca++ and Mg++
Expressed Sequence:
SNAEDPQGDAAQKTDTSHHDQDHPTFNKITPNLAEFAFSLYRQLAHQSNSTNIFFSPVSIATAFAML
SLGTKADTHDEILEGLNFNLTEIPEAQIHEGFQELLRTLNQPDSQLQLTTGNGLFLSEGLKLVDKFL
EDVKKLYHSEAFTVNFGDTEEAKKQINDYVEKGTQGKIVDLVKELDRDTVFALVNYIFFKGKWERPF
EVKDTEEEDFHVDQATTVKVPMMKRLGMFNIQHCKKLSSWVLLMKYLGNATAIFFLPDEGKLQHLEN
ELTHDIITKFLENEDRRSASLHLPKLSITGTYDLKSVLGQLGITKVFSNGADLSGVTEEAPLKLSKA
VHKAVLTIDEKGTEAAGAMFLEAIPMSIPPEVKFNKPFVFLMIEQNTKSPLFMGKVVNPTQK
Native Sequence:
MPSSVSWGILLLAGLCCLVPVSLAEDPQGDAAQKTDTSHHDQDHPTF
NKITPNLAEFAFSLYRQLAHQSNSTNIFFSPVSIATAFAMLSLGTKA
DTHDEILEGLNFNLTEIPEAQIHEGFQELLRTLNQPDSQLQLTTGNG
LFLSEGLKLVDKFLEDVKKLYHSEAFTVNFGDTEEAKKQINDYVEKG
TQGKIVDLVKELDRDTVFALVNYIFFKGKWERPFEVKDTEEEDFHVD
QVTTVKVPMMKRLGMFNIQHCKKLSSWVLLMKYLGNATAIFFLPDEG
KLQHLENELTHDIITKFLENEDRRSASLHLPKLSITGTYDLKSVLGQ
LGITKVFSNGADLSGVTEEAPLKLSKAVHKAVLTIDEKGTEAAGAMF
LEAIPMSIPPEVKFNKPFVFLMIEQNTKSPLFMGKVVNPTQK
Calculated Isoelectric Point:
5.37
Molecular Weight:
44569
Last Updated:
11/14/2012

Links

Characterization Data

Gel

Click to enlarge image Protein Array in which CPTC-SERPINA1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Click to enlarge

CPTC-SERPINA1 PAGE

PAGE of Ag 11019 (with molecular weight standards in lane 1)

SOPs

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