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Metastasin 100 calcium-binding protein A4 (Calvasculin)

Antibody: CPTC-S100A4-1

Background

Catalog Number:
CPTC-S100A4-1
Target Antigen:
Metastasin 100 calcium-binding protein A4 (Calvasculin)
Isotype:
IgG2a
Species:
Mouse
Last Updated:
01/24/2012
Antigen Recognition(s):
recombinant full-length

Purchase

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot Using CPTC-S100A4-1 as primary Ab against Ag 10337(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).
Click to enlarge

CPTC-S100A4-1 Western Blot

Western Blot Using CPTC-S100A4-1 as primary Ab against Ag 10337(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).

Indirect ELISA

Click to enlarge image Indirect ELISA of CPTC-S100A4-1
Click to enlarge

CPTC-S100A4-1 Indirect ELISA

Indirect ELISA of CPTC-S100A4-1

NCI 60 Protein Array

Click to enlarge image Protein Array in which S100A4-1 is screen agains the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5.
Click to enlarge

CPTC-S100A4-1 NCI60 Protein Array

Protein Array in which S100A4-1 is screen agains the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5.

IHC NCI60

Click to enlarge image Immunohistochemistry of CPTC-S100A4-1 for NCI60 Cell Line Array. Data scored as:
0=NEGATIVE
1=WEAK (red)
2=MODERATE (blue)
3=STRONG (green)
Titer: 1:15000
Click to enlarge

CPTC-S100A4-1 IHC NCI60

Immunohistochemistry of CPTC-S100A4-1 for NCI60 Cell Line Array. Data scored as:
0=NEGATIVE
1=WEAK (red)
2=MODERATE (blue)
3=STRONG (green)
Titer: 1:15000

Click to view IHC NCI60 Cell Line Array example

IHC Tissue

Click to enlarge image Tissue Micro-Array(TMA) core of ovarian cancer showing cytoplasmic staining using Antibody CPTC-S100A4-1. Titer: 1:15000
Click to enlarge

CPTC-S100A4-1 IHC Tissue

Tissue Micro-Array(TMA) core of ovarian cancer showing cytoplasmic staining using Antibody CPTC-S100A4-1. Titer: 1:15000

SPR

Click to enlarge image Kinetic titration data for S100A4-1 Ab (635A8.1) using Biacore SPR method
Click to enlarge

CPTC-S100A4-1 SPR

Kinetic titration data for S100A4-1 Ab (635A8.1) using Biacore SPR method

Immuno-MS

Download S100A4-1.pdf (200.3 KB)

CPTC-S100A4-1 Immuno MS

This PDF contains the spectra and recommendation for use of this antibody in immuno capture mass spectrometry.

Antibody: CPTC-S100A4-2

Background

Catalog Number:
CPTC-S100A4-2
Target Antigen:
Metastasin 100 calcium-binding protein A4 (Calvasculin)
Isotype:
IgG2a
Species:
Mouse
Last Updated:
01/24/2012
Antigen Recognition(s):
recombinant full-length

Purchase

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot Using CPTC-S100A4-2 as primary Ab against Ag 10337(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).
Click to enlarge

CPTC-S100A4-2 Western Blot

Western Blot Using CPTC-S100A4-2 as primary Ab against Ag 10337(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).

Indirect ELISA

Click to enlarge image Indirect ELISA of CPTC-S100A4-2
Click to enlarge

CPTC-S100A4-2 Indirect ELISA

Indirect ELISA of CPTC-S100A4-2

NCI 60 Protein Array

Click to enlarge image Protein Array in which CPTC-AKR1B1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Click to enlarge

CPTC-S100A4-2 NCI60 Protein Assay

Protein Array in which CPTC-AKR1B1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

IHC NCI60

Click to enlarge image Immuno-histochemistry of CPTC-S100A4-2 for NCI60  Cell Line Array at titer 1:8000
0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green)
Click to enlarge

CPTC-S100A4-2 IHC NCI60

Immuno-histochemistry of CPTC-S100A4-2 for NCI60 Cell Line Array at titer 1:8000
0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green)

Click to view IHC NCI60 Cell Line Array example

IHC Tissue

Click to enlarge image Tissue Micro-Array(TMA) core of ovarian cancer showing cytoplasmic staining using Antibody CPTC-S100A4-2. Titer: 1:8000
Click to enlarge

CPTC-S100A4-2 IHC Tissue

Tissue Micro-Array(TMA) core of ovarian cancer showing cytoplasmic staining using Antibody CPTC-S100A4-2. Titer: 1:8000

IHC HPA

Download S100A4-2 HPA Report.pdf (558.4 KB)

CPTC-S100A4-2 evaluation by the Human Protein Atlas

This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).

SPR

Click to enlarge image Kinetic titration data for S100A4-2 Ab (635A11.1) using Biacore SPR method
Click to enlarge

CPTC-S100A4-2 SPR

Kinetic titration data for S100A4-2 Ab (635A11.1) using Biacore SPR method

Immuno-MS

Download S100A4-2.pdf (199.1 KB)

CPTC-S100A4-2 Immuno-MS

This PDF contains the spectra and recommendation for use of this antibody in immuno capture mass spectrometry.

Antibody: CPTC-S100A4-3

Background

Catalog Number:
CPTC-S100A4-3
Target Antigen:
Metastasin 100 calcium-binding protein A4 (Calvasculin)
Isotype:
IgG2a
Species:
Mouse
Last Updated:
03/07/2013
Antigen Recognition(s):

Purchase

External Links

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot Using CPTC-S100A4-3 as primary Ab against Ag 10337(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).
Click to enlarge

CPTC-S100A4-3 Western Blot

Western Blot Using CPTC-S100A4-3 as primary Ab against Ag 10337(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).

Indirect ELISA

Click to enlarge image Indirect ELISA of CPTC-S100A4-3
Click to enlarge

CPTC-S100A4-3 Indirect ELISA

Indirect ELISA of CPTC-S100A4-3

NCI 60 Protein Array

Click to enlarge image Protein Array in which CPTC-S100A4-3  is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Click to enlarge

CPTC-S100A4-3 NCI 60 Protein Array

Protein Array in which CPTC-S100A4-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

IHC NCI60

Click to enlarge image Immuno-histochemistry of CPTC-S100A4-3 for NCI60  Cell Line Array
0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green)
Titer: 1:7500
Click to enlarge

CPTC-S100A4-3 IHC NCI60

Immuno-histochemistry of CPTC-S100A4-3 for NCI60 Cell Line Array
0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green)
Titer: 1:7500

Click to view IHC NCI60 Cell Line Array example

IHC Tissue

Click to enlarge image Tissue Micro-Array(TMA) core of ovarian cancer showing cytoplasmic staining using Antibody CPTC-S100A4-3. Titer: 1:7500
Click to enlarge

CPTC-S100A4-3 IHC Tissue

Tissue Micro-Array(TMA) core of ovarian cancer showing cytoplasmic staining using Antibody CPTC-S100A4-3. Titer: 1:7500

IHC HPA

Download S100A4-3 HPA Report.pdf (161.4 KB)

CPTC-S100A4-3 evaluation by the Human Protein Atlas

This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).

IHC HPA

Download CPTC-S100A4-3.pdf (107.0 KB)

CPTC-S100A4-3 Evaluation by Human Protein Atlas

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)

Array

SPR

Click to enlarge image Kinetic titration data for S100A4-3 Ab (635A23.1) using Biacore SPR method
Click to enlarge

CPTC-S100A4-3 SPR

Kinetic titration data for S100A4-3 Ab (635A23.1) using Biacore SPR method

Immuno-MS

Download S100A4-3.pdf (202.1 KB)

CPTC-S100A4-3 Immuno-MS

This PDF contains the spectra and recommendation for use of this antibody in immuno capture mass spectrometry.

Antigen: Metastasin 100 calcium-binding protein A4 (Calvasculin)

Background

NCI Identification Number:
10337
Antigen Name:
Metastasin 100 calcium-binding protein A4 (Calvasculin)
CPTC Name:
CPTC-S100A4
Keywords:
S100A4; 18A2; 42A; CAPL; Calvasculin; FSP1; MTS1; Metastasin; OTTHUMP00000015467; OTTHUMP00000015468; OTTHUMP00000015469; OTTHUMP00000032895; P9KA; PEL98
Function:
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Chromosomal Localization:
1q21
Expression System:
E. Coli
NCBI Accession Number:
M80563
Swiss Prot Accession Number:
P26447
DNA Source:
HIP:HsCD00004965
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
3105
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMACPLEKALDVMVSTFHK
YSGKEGDKFKLNKSELKELLT
RELPSFLGKRTDEAAFQKLMS
NLDSNRDNEVDFQEYCVFLSC
IAMMCNEFFEGFPDKQPRKK
Native Sequence:
MACPLEKALDVMVSTFHKYSG
KEGDKFKLNKSELKELLTREL
PSFLGKRTDEAAFQKLMSNLD
SNRDNEVDFQEYCVFLSCIAM
MCNEFFEGFPDKQPRKK
Calculated Isoelectric Point:
5.84
Molecular Weight:
12001
Last Updated:
11/14/2012

Links

Characterization Data

Gel

Click to enlarge image Kinetic titration data for S100A4-3 Ab (635A23.1) using Biacore SPR method
Click to enlarge

Ag 10337

PAGE of Ag 10337 with molecular weight standards

SOPs

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