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protein tyrosine phosphatase, non-receptor type 6

Antibody: CPTC-PTPN6-1

Background

Catalog Number:
CPTC-PTPN6-1
Target Antigen:
protein tyrosine phosphatase, non-receptor type 6
Isotype:
IgG2a
Species:
Mouse
Last Updated:
05/22/2014
Antigen Recognition(s):
recombinant full-length

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Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-PTPN6-1 as primary Ab against PTPN6 (rAg 10766) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

CPTC-PTPN6-1 Western blot

Western Blot using CPTC-PTPN6-1 as primary Ab against PTPN6 (rAg 10766) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Click to enlarge

CPTC-PTPN6-1 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.

NCI 60 Protein Array

CPTC-PTPN6-1 NCI 60 Protein Array

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.

Antibody: CPTC-PTPN6-2

Background

Catalog Number:
CPTC-PTPN6-2
Target Antigen:
protein tyrosine phosphatase, non-receptor type 6
Isotype:
IgG2a
Species:
Mouse
Last Updated:
05/22/2014
Antigen Recognition(s):
recombinant full-length

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Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-PTPN6-2 as primary Ab against PTPN6 (rAg 10766) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

CPTC-PTPN6-2 Western blot

Western Blot using CPTC-PTPN6-2 as primary Ab against PTPN6 (rAg 10766) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Click to enlarge

CPTC-PTPN6-2 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.

NCI 60 Protein Array

CPTC-PTPN6-2 NCI 60 Protein Array

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.

Antibody: CPTC-PTPN6-3

Background

Catalog Number:
CPTC-PTPN6-3
Target Antigen:
protein tyrosine phosphatase, non-receptor type 6
Isotype:
IgG2b
Species:
Mouse
Last Updated:
05/22/2014
Antigen Recognition(s):
recombinant full-length

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Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-PTPN6-3 as primary Ab against PTPN6 (rAg 10766) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

CPTC-PTPN6-3 Western blot

Western Blot using CPTC-PTPN6-3 as primary Ab against PTPN6 (rAg 10766) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Click to enlarge

CPTC-PTPN6-3 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.

NCI 60 Protein Array

CPTC-PTPN6-3 NCI 60 Protein Array

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.

Antigen: protein tyrosine phosphatase, non-receptor type 6

Background

NCI Identification Number:
10766
Antigen Name:
protein tyrosine phosphatase, non-receptor type 6
CPTC Name:
CPTC-PTPN6
Keywords:
PTP-1C; HCP; HCPH; SHP1; SHP-1; Hematopoietic cell protein-tyrosine phosphatase; Protein-tyrosine phosphatase 1C; Protein-tyrosine phosphatase SHP-1; SH-PTP1; hematopoietic cell phosphatase; tyrosine-protein phosphatase non-receptor type 6; SHP-1L; HPTP1C; PTP1C; EC 3.1.3.48
Function:
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which
act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP
is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways
in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of
phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported.

Plays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other
signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular
components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation.

Protein tyrosine phosphatases (PTPs) are a group of enzymes that catalyze the removal of phosphate groups from tyrosine residues by the hydrolysis of phosphoric acid monoesters. They directly oppose the actions of kinases and phosphorylases and therefore play an integral role in many signal transduction pathways. PTPs are cysteine-dependent phosphatases that contain a conserved C[X]5R motif essential for enzymatic activity. PTPs are currently divided into five subtypes; tyrosine-specific phosphatases (e.g. PTP1B), dual specificity phosphatases (DSPs) (e.g. DUSP1), Cdc25 phosphatases (e.g. Cdc25A), myotubularin-related phosphatases (e.g. MTMR13) and low molecular weight phosphatases (e.g. PTPase A). Perturbations in PTP activity has been implicated in human diseases, including type II diabetes. PTPs have been identified as a negative regulator of the insulin signaling by dephosphorylating phosphotyrosine residues of insulin receptor kinase. In cancers, PTPs dephosphorylate EGFR, JAK2 and TYK2 kinases, promoting oncogenic transformation.
Chromosomal Localization:
12p13.31
Expression System:
E. Coli
NCBI Accession Number:
BC007667
Swiss Prot Accession Number:
P29350
DNA Source:
HsCD00003674
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG19
Extinction Coefficient:
86938
Buffers:
PBS without Ca++ and Mg++
Expressed Sequence:
SNAMLSRGWFHRDLSGLDAETLLKGRGVHGSFLARPSRKNQGDFSLSVRVGDQVTHIRIQ
NSGDFYDLYGGEKFATLTELVEYYTQQQGVLQDRDGTIIHLKYPLNCSDPTSERWYHGHM
SGGQAETLLQAKGEPWTFLVRESLSQPGDFVLSVLSDQPKAGPGSPLRVTHIKVMCEGGR
YTVGGLETFDSLTDLVEHFKKTGIEEASGAFVYLRQPYYATRVNAADIENRVLELNKKQE
SEDTAKAGFWEEFESLQKQEVKNLHQRLEGQRPENKGKNRYKNILPFDHSRVILQGRDSN
IPGSDYINANYIKNQLLGPDENAKTYIASQGCLEATVNDFWQMAWQENSRVIVMTTREVE
KGRNKCVPYWPEVGMQRAYGPYSVTNCGEHDTTEYKLRTLQVSPLDNGDLIREIWHYQYL
SWPDHGVPSEPGGVLSFLDQINQRQESLPHAGPIIVHCSAGIGRTGTIIVIDMLMENIST
KGLDCDIDIQKTIQMVRAQRSGMVQTEAQYKFIYVAIAQFIETTKKKLEVLQSQKGQESE
YGNITYPPAMKNAHAKASRTSSKHKEDVYENLHTKNKREEKVKKQRSADKEKSKGSLKRK
Native Sequence:
MLSRGWFHRDLSGLDAETLLKGRGVHGSFLARPSRKNQGDFSLSVRVGDQVTHIRIQNSG
DFYDLYGGEKFATLTELVEYYTQQQGVLQDRDGTIIHLKYPLNCSDPTSERWYHGHMSGG
QAETLLQAKGEPWTFLVRESLSQPGDFVLSVLSDQPKAGPGSPLRVTHIKVMCEGGRYTV
GGLETFDSLTDLVEHFKKTGIEEASGAFVYLRQPYYATRVNAADIENRVLELNKKQESED
TAKAGFWEEFESLQKQEVKNLHQRLEGQRPENKGKNRYKNILPFDHSRVILQGRDSNIPG
SDYINANYIKNQLLGPDENAKTYIASQGCLEATVNDFWQMAWQENSRVIVMTTREVEKGR
NKCVPYWPEVGMQRAYGPYSVTNCGEHDTTEYKLRTLQVSPLDNGDLIREIWHYQYLSWP
DHGVPSEPGGVLSFLDQINQRQESLPHAGPIIVHCSAGIGRTGTIIVIDMLMENISTKGL
DCDIDIQKTIQMVRAQRSGMVQTEAQYKFIYVAIAQFIETTKKKLEVLQSQKGQESEYGN
ITYPPAMKNAHAKASRTSSKHKEDVYENLHTKNKREEKVKKQRSADKEKSKGSLKRK
Calculated Isoelectric Point:
7.65
Molecular Weight:
67992
Last Updated:
11/14/2012

Links

Characterization Data

Gel

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Click to enlarge

CPTC-PTPN6 PAGE

PAGE of PTPN6 (rAg 10766) with molecular weight standards in lane 1

SOPs

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