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Phosphoserine phosphatase-like

Antibody: CPTC-PSPHL-1

Background

Catalog Number:
CPTC-PSPHL-1
Target Antigen:
Phosphoserine phosphatase-like
Isotype:
IgG2b
Species:
Mouse
Last Updated:
01/24/2012
Antigen Recognition(s):
recombinant full-length

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Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-PSPHL-1 as primary Ab against PSPHL (Ag 00002) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

FSAI003R-3G9 Western blot

Western Blot using CPTC-PSPHL-1 as primary Ab against PSPHL (Ag 00002) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

FSAI003R-3G9 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

NCI 60 Protein Array

Click to enlarge image Protein Array in which CPTC-PSPHL-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Click to enlarge

CPTC-PSPHL-1 Protein Array

Protein Array in which CPTC-PSPHL-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

Characterization SOP Files

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Antibody: CPTC-PSPHL-2

Background

Catalog Number:
CPTC-PSPHL-2
Target Antigen:
Phosphoserine phosphatase-like
Isotype:
IgG2b
Species:
Mouse
Last Updated:
02/12/2013
Antigen Recognition(s):

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Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-PSPHL-2 as primary Ab against PSPHL (Ag 00002) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

FSAI003R-3E10 Western blot

Western Blot using CPTC-PSPHL-2 as primary Ab against PSPHL (Ag 00002) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

FSAI003R-3E10 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

NCI 60 Protein Array

Click to enlarge image Protein Array in which CPTC-PSPHL-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Click to enlarge

CPTC-PSPHL-2 Protein Array

Protein Array in which CPTC-PSPHL-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

Characterization SOP Files

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Antibody: CPTC-PSPHL-3

Background

Catalog Number:
CPTC-PSPHL-3
Target Antigen:
Phosphoserine phosphatase-like
Isotype:
IgG2b
Species:
Mouse
Last Updated:
02/28/2013
Antigen Recognition(s):
recombinant full-length

Purchase

Characterization Data [Compare Characterization Data]

Western Blot

Click to enlarge image Western Blot using CPTC-PSPHL-3 as primary Ab against PSPHL (Ag 00002) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Click to enlarge

FSAI003R-2F10 Western blot

Western Blot using CPTC-PSPHL-3 as primary Ab against PSPHL (Ag 00002) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

Characterization SOP Files

Indirect ELISA

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

FSAI003R-2F10 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Characterization SOP Files

NCI 60 Protein Array

CPTC-PSPHL-3 NCI 60 Protein Array

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.

Characterization SOP Files

IHC NCI60

CPTC-PSPHL-3 IHC NCI60

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Click to view IHC NCI60 Cell Line Array example

Characterization SOP Files

IHC Tissue

CPTC-PSPHL-3 IHC Tissue

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Characterization SOP Files

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Antigen: Phosphoserine phosphatase-like

Background

NCI Identification Number:
00002
Antigen Name:
Phosphoserine phosphatase-like
CPTC Name:
CPTC-PSPHL
Keywords:
phosphoserine phosphatase-like; CO9; L-3-phosphoserine-phosphatase homolog
Function:
This gene is significantly upregulated in Fanconi's anemia fibroblasts but downregulated or absent in fibroblasts
from normal donors. It is also highly expressed in FA B-cells of complementation group A.
Chromosomal Localization:
7p11.2
Expression System:
E. coli
NCBI Accession Number:
XM_926720
Swiss Prot Accession Number:
O15172
DNA Source:
xxx
Immunogen:
Recombinant Full Length Protein
Vector Name:
pDest 527
Extinction Coefficient:
Buffers:
Expressed Sequence:
MRSGSHHHHHHRSDI
TSLYKKVGENLYFQG
ASASCSPGGALASPE
PGRKILPRMISHSEL
RKLFYSADAVCF
Native Sequence:
Calculated Isoelectric Point:
7.8
Molecular Weight:
11200
Last Updated:
11/14/2012

Links

Characterization Data

Gel

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click to enlarge

CPTC-PSPHL PAGE

PAGE of Ag 00002 (PSPHL) (with molecular weight standards in lane 1)

SOPs

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