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Peroxiredoxin 2 Peptide 1

Antibody: CPTC-PRDX2-1
Background

Catalog Number:

CPTC-PRDX2-1

RRID:

AB_2617315

Target Antigen:

Peroxiredoxin 2 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (145.3 KB)

CPTC-PRDX2-1 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-PRDX2-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-727) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from plasma. Click image to enlarge

CPTC-PRDX2-1 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-PRDX2-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-727)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from plasma.


Characterization SOP Files

  Immunofluorescence

CPTC-PRDX2-1 Immunofluorescence

Result: Negative

Immunofluorescence staining of MCF7 cells using CPTC-PRDX2-1 antibody. PRDX2 protein expression was not detected.


Characterization SOP Files

  Immunofluorescence - HPA
Click to enlarge image This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line THP-1 shows localization to cytosol. Human assay: THP-1 fixed with PFA, dilution: 1:100 Human assay: U2OS fixed with PFA, dilution: 1:100 Click image to enlarge

CPTC-PRDX2-1 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line THP-1 shows localization to cytosol. Human assay: THP-1 fixed with PFA, dilution: 1:100
Human assay: U2OS fixed with PFA, dilution: 1:100


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-PRDX2-1 Indirect ELISA (peptide)

Result: Positive

Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to full-length Antigen coated on plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-PRDX2-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to full-length Antigen coated on plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Western Blot - HPA - tissue or cell lysate
Click to enlarge image This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present. Analysis performed using a standard panel of samples. Antibody Dilution 1:500 Click image to enlarge

CPTC-PRDX2-1 Evaluation by the Human Protein Atlas

Result: Presumed Positive (with additional bands)

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present.
Analysis performed using a standard panel of samples. Antibody Dilution 1:500


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-PRDX2-1 as primary Ab against full-length recombinant Ag 10333 (lane 2). Also included are molecular wt. standards (lane 1) and the PRDX2-1 Ab as positive control (lane 3). Click image to enlarge

CPTC-PRDX2-1 Western Blot

Result: Positive

Western Blot using CPTC-PRDX2-1 as primary Ab against full-length recombinant Ag 10333 (lane 2). Also included are molecular wt. standards (lane 1) and the PRDX2-1 Ab as positive control (lane 3).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-PRDX2-1 as primary Ab against lysates from six breast cancer cell lines from the NCI60 cell line collection (lanes 2-7). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-PRDX2-1 NCI60 Western blot

Result: Positive

Western Blot using CPTC-PRDX2-1 as primary Ab against lysates from six breast cancer cell lines from the NCI60 cell line collection (lanes 2-7). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

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Antigen: Peroxiredoxin 2 Peptide 1
Background

NCI Identification Number:

00043

Antigen Name:

Peroxiredoxin 2 Peptide 1

CPTC Name:

CPTC-PRDX2 Peptide 1

Aliases:

NKEFB; PRP; TDPX1; TSA; PRX2; PRXII; Natural Killer Cell-Enhancing Factor B; Thioredoxin-Dependent Peroxide Reductase 1; NKEF-B; EC 1.11.1.15; MGC4104; PTX1; TPX1; Peroxiredoxin-2; Thiol-Specific Antioxidant 1; Torin; EC 1.11.1

Function:

This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. The crystal structure of this protein has been resolved to 2.7 angstroms. Transcript variants encoding distinct isoforms have been identified for this gene.

Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the
thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).

Chromosomal Localization:

19p13.2

Accession Number:

NP_005800.3

UniProt Accession Number:

P32119

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

LSEDYGVLK

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

990

Last Updated:

06/21/2013

Links

Characterization Data

SOPs

No SOPs available.

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